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A New Screening Method for the Selection of Calla Lily Zantedeschia aethiopica Cultivars Resistant to Calla Lily Soft Rot

칼라의 무름병 저항성 품종 선발을 위한 검정 방법 개발

  • Joung, Hyang Young (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Choi, Mok Pil (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Han, Kyung Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science) ;
  • Kim, Su (Vegetable Research Division, National Institute of Horticultural & Herbal Science) ;
  • Goo, Dae Hoe (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Kang, Yun Im (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Choi, Youn Jung (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Park, Sang Kun (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
  • 정향영 (국립원예특작과학원 화훼과) ;
  • 최목필 (국립원예특작과학원 화훼과) ;
  • 한경숙 (국립원예특작과학원 원예특작환경과) ;
  • 김수 (국립원예특작과학원 채소과) ;
  • 구대회 (국립원예특작과학원 화훼과) ;
  • 강윤임 (국립원예특작과학원 화훼과) ;
  • 최윤정 (국립원예특작과학원 화훼과) ;
  • 박상근 (국립원예특작과학원 화훼과)
  • Received : 2012.11.07
  • Accepted : 2013.01.23
  • Published : 2013.06.30

Abstract

This experiment was carried out to establish the screening methods for the selection of Zantedeschia aethiopica cultivars resistant to calla lily soft rot caused by Pectobacterium carotovorum subsp. carotovorum. A center of leaf and petiole tissues of four Z. aethiopica cultivars were wounded and inoculated with three different inoculum concentrations, $1{\times}10^7cfu/mL$, $1{\times}10^8cfu/mL$, and $1{\times}10^9cfu/mL$, of EccNHRI-21 isolate. And they were evaluated at 4, 18, and 26 hours after inoculation. The lesion of maceration was developed well in the leaf tissues inoculated with $1{\times}10^9cfu/mL$ concentration. And evaluation of resistance in 18 hours after inoculation was correlated with field resistance positively. Using this method, ten Z. aethiopica commercial cultivars and four wild types were screened. 'Crowbrough' and 'White Cutie' were selected as highly resistant genotypes and 'Mont Blanc' and 'Silky White' as resistant genotypes. 'Wedding March' and 'Kiwi Blush' were evaluated as moderate resistant and the others including 'Childsiana' were susceptible. And all of four wild types were evaluated as more than moderate resistant. In spite of control through cultural, biological and chemical methods, the use of resistant cultivars is most efficient to overcome calla lily soft rot. This newly developed screening method was helpful for breeding new varieties resistant to calla lily soft rot.

본 연구는 P. carotovorum subsp. carotovorum에 의해 발생하는 칼라의 무름병 저항성 품종 선발을 위한 효율적인 검정 방법을 구명하기 위해 수행되었다. Z. aethiopica 4품종에 대하여 $1{\times}10^7cfu/mL$, $1{\times}10^8cfu/mL$, $1{\times}10^9cfu/mL$ 농도의 EccNHRI-21 균주 현탁액을 잎과 엽병에 상처접종하고, 접종 후 4, 18, 26시간 후에 무름병 발생 정도를 조사하였다. 그 결과, $1{\times}10^9cfu/mL$ 농도의 균주 현탁액을 잎에 접종한 후 18시간 이후에 저항성 정도를 평가하는 것이 칼라 무름병 저항성 검정 및 품종 선발을 위해 가장 적합한 것으로 나타났다. 상처접종법을 이용하여 Z. aethiopica 재배품종 10종과 야생종 4종 등 총 14종에 대하여 무름병 저항성 정도를 평가한 결과, 재배품종의 경우 'Crowbrough'와 'White Cutie'가 가장 강한 저항성을 보였으며, 'Mont Blanc'과 'Silky White'도 저항성 품종으로 선발되었다. 또한 'Wedding March'와 'Kiwi blush'는 중도저항성 품종으로 평가되었고, 'Childsiana' 등 4품종은 감수성 품종으로 평가되었다. 야생종의 경우 'Z. aethiopica wildtype 11'이 저항성을 보이는 등 4종 모두 중도저항성 이상의 무름병 저항성을 가지는 것으로 평가되었다. 지금까지 경종적 방제 및 생물학적 방제, 화학적 방제 등 여러 가지 방제 방법이 시도되었으나, 무름병 발생 피해를 최소화하기 위해서는 내병성 품종을 재배하는 것이 가장 효율적이다. 따라서 상처접종법을 이용한 무름병 저항성 검정 기술이 칼라의 무름병 저항성 품종 개발에 유용하게 쓰일 것으로 기대된다.

Keywords

References

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