Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of Plant Growth Regulators on Plant Regeneration and Direct Shoots Formation of Petal Explants of Chrysanthemum Flower Color Mutants Varieties, 'ARTI-purple' and 'ARTI-queen'

국화 화색 돌연변이 품종 'ARTI-purple' 및 'ARTI-queen' 꽃잎 조직의 재분화와 신초형성에 미치는 식물생장호르몬의 영향

  • Lee, Yu-Mi (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Eun Jeong (Research Safety Management Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sung, Sang Yeop (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ha, Bo-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 이유미 (한국원자력연구원 첨단방사선연구소) ;
  • 강은정 (한국생명공학연구원 연구안전관리지원센터) ;
  • 성상엽 (한국원자력연구원 첨단방사선연구소) ;
  • 김상훈 (한국원자력연구원 첨단방사선연구소) ;
  • 하보근 (한국원자력연구원 첨단방사선연구소) ;
  • 김동섭 (한국원자력연구원 첨단방사선연구소) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소) ;
  • 강시용 (한국원자력연구원 첨단방사선연구소)
  • Received : 2012.11.21
  • Accepted : 2013.03.03
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Chrysanthemum is one of the most popular ornamental plants worldwide. Recently, lots of new and novel chrysanthemum varieties have been developed using mutagenesis. However, there was no study for comparison of tissue culture condition among the mutant varieties derived from one original variety, until now. This study was conducted to compare the efficient regeneration condition of the two chrysanthemum mutant varieties, 'ARTI-purple' and 'ARTI-queen'. Two different flower parts (disk and ray florets) at the unopened and early blooming stages were used for comparison of regeneration condition on MS medium supplemented with combinations of three growth regulators (BA, NAA, and IAA). The highest regeneration rate was identified on the NAA and BA combination when the disk florets at unopened blooming stage are used. The best optimum combinations of growth regulators were identified as NAA $1.0mg{\cdot}L^{-1}$ and BA $0.5mg{\cdot}L^{-1}$ at 'ARTI-purple', which displayed 47.9% regeneration. However, regeneration of 'ARTI-queen' was the highest as 25.6% at NAA $2.0mg{\cdot}L^{-1}$ and BA $1.0mg{\cdot}L^{-1}$. There results indicate that there is a difference for the optimum regeneration condition between the mutant varieties derived from one original variety. These results will be useful for construction of efficient regeneration system of diverse chrysanthemum mutants developed by mutation breeding.

국화는 전 세계적으로 가장 대중적인 화훼류 중의 하나로써, 최근 새로운 국화 품종들이 돌연변이육종을 통해 개발되고 있다. 그러나 현재까지 하나의 국화 원품종에서 유래한 돌연변이 품종들 간의 조직배양 조건 차이에 대한 보고는 전무하다. 따라서 본 연구에서는 2개의 국화 화색 돌연변이 품종 'ARTI-purple'과 'ARTI-queen'의 효율적인 재분화 조건을 비교하기 위해 수행되었다. 실험재료로 꽃봉오리 시기와 통상화 개화 이전 시기의 꽃잎을 이용하였고, 꽃의 조직에 따른 차이를 확인하기 위해 통상화 및 설상화를 구분하여 실험을 수행하였다. 국화 재분화를 위한 식물생장호르몬의 적정 조합을 찾기 위해 3% sucrose, 0.3% gelrite를 포함한 MS 배지에 BA, NAA, IAA 중 2가지 호르몬을 조합별로 첨가하여 실험을 수행하였다. 절편체는 일장 16시간, 온도 $25{\pm}1^{\circ}C$ 조건으로 배양하였으며, 재분화율 조사는 배양 후 4주 및 8주차에 실시하였다. 결과적으로, 가장 높은 재분화율은 두 품종 모두 최적 NAA와 BA의 호르몬 조합에서 꽃봉오리 시기의 통상화를 사용할 시 가장 높게 확인되었다. 재분화를 위한 식물생장호르몬의 최적 조합은 'ARTI-purple'의 경우 NAA $1.0mg{\cdot}L^{-1}$와 BA $0.5mg{\cdot}L^{-1}$로 47.9%의 재분화 효율을 보였으며, 'ARTI-queen'의 경우 NAA $2.0mg{\cdot}L^{-1}$와 BA $1.0mg{\cdot}L^{-1}$로 25.6%의 재분화 효율을 나타내었다. 재분화된 지상부는 1/2 MS배지에서 발근시켰으며, 기내 소식물체는 유리온실에서 성공적으로 순화되었다. 본 연구결과는 감마선을 사용한 돌연변이육종법에 의해 개발된 다양한 국화품종의 효율적인 재분화 시스템을 구축하는데 유용한 정보를 제공할 것이다.

Keywords

References

  1. Annadana, S., W. Rademaker, M. Ramanna, M. Udayakumar, and J. de Jong. 2000. Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum. Plant Cell Tissue Organ Cult. 62:47-55. https://doi.org/10.1023/A:1006483414260
  2. Broertjes, C. 1966. Mutation breeding of chrysanthemums. Euphytica 15:156-162. https://doi.org/10.1007/BF00022318
  3. Chakrabarty, D., A.K.A. Mandal, and S.K. Datta. 2000. SEM and light microscopic studies on direct shoot regeneration from ray florets of chrysanthemum. Isr. J. Plant Sci. 48:105-107. https://doi.org/10.1560/B6WM-2565-2BVY-P3W9
  4. Datta, S.K., D. Chakrabarty, and A.K.A. Mandal. 2001. Gamma ray-induced genetic manipulations in flower colour and shape in Dendranthema grandiflorum and their management through tissue culture. Plant Breed. 120:91-92. https://doi.org/10.1046/j.1439-0523.2001.00553.x
  5. Gilissen, L.J.W., M.J. van Staveren, J.C. Hakkert, and M.J.M. Smulders. 1996. Competence for regeneration during tobacco internodal development: Involvement of plant age, cell elongation stage, and degree of polysomaty. Plant Physiol. 111:1243-1250.
  6. Han, B.H., S.Y. Lee, and B.M. Park. 2009. Comparison of chrysanthemum cultivars based on direct shoot regeneration rates in tissue culture. J. Plant Biotechnol. 36:275-280. https://doi.org/10.5010/JPB.2009.36.3.275
  7. Himstedt, J.P., H.J. Jacobsen, and G. Fisher-Kluver. 2001. Shoot regeneration from stem and leaf explants of chrysanthemum (Dendranthema x grandiflorum). Acta Hortic. 560:421-424.
  8. Hoque, M.I., M.T. Jahan, and R.H. Sarker. 1998. In vitro shoot regeneration and ex vitro rooting in Chrysanthemum morifolium Ramat. Plant Tissue Cult. 8:157-164.
  9. Jaime, A. and T. da Silva. 2003. Chrysanthemum: Advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnol. Adv. 21:751-766.
  10. Jeong, J.H., D. Chakrabarty, S.J. Kim, and K.Y. Paek. 2002. Transformation of chrysanthemum (Dendranthema grandiflorum Kitamura cv. Cheonsu) by constitutive expression of rice OsMADS1 gene. J. Kor. Soc. Hort. Sci. 43:382-386.
  11. Jevremović, S. and L.J. Radojevic. 2004. Mass production of different chrysanthemum (Chrysanthemum morifolium) cultivars by culture in vitro. J. Sci. Agric. Res. 65:47-54.
  12. Kang, C.H., S.J. Yun, B.S. Han, G.J. Lee, K.H. Choi, J.S. Park, and Y.K. Shin. 2011. Development of salt-tolerant transgenic chrysanthemum (Dendranthema grandiflorum) lines and bioassay with a change of cell specificity. J. Plant Biotechnol. 38:1-8. https://doi.org/10.5010/JPB.2011.38.1.001
  13. Kumar, B., S. Kumar, and M. Thakur. 2012. In vitro mutation induction and selection of chrysanthemum (Dendranthema grandiflora Tzelev) lines with improved resistance to Septoria Obesa Syd. Int. J. Plant Res. 2:103-107. https://doi.org/10.5923/j.plant.20120204.01
  14. Kumar, S., K.V. Prasad, and M.L. Choudhary. 2006. Detection of genetic variability among chrysanthemum radiomutants using RAPD markers. Current Sci. 90:1108-1113.
  15. Lu, C., I.K. Vasil, and P. Ozias-Akins. 1982. Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62:109-112. https://doi.org/10.1007/BF00293341
  16. Malaure, R.S., M.R. Davey, and J.B. Power. 1989. Isolation and culture of protoplasts of Chrysanthemum cinerariaefolium Vis. Pyrethrum Post 17:90-94.
  17. Mandal, A.K.A. and S.K. Datta. 2005. Direct somatic embryogenesis and plant regeneration from ray florets of chrysanthemum. Biol. Plant. 49:29-33. https://doi.org/10.1007/s10535-005-0033-6
  18. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473-479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  19. Nahid, J.S., S. Shyamali, and H. Kazumi. 2007. High frequency shoot regeneration from petal explants of Chrysanthemum morifolium Romat. in vitro. Pak. J. Biol. Sci. 10:3356-3361. https://doi.org/10.3923/pjbs.2007.3356.3361
  20. Park, I.S., G.J. Lee, D.S. Kim, S.J. Chung, J.B. Kim, H.S. Song, D.H. Goo, and S.Y. Kang. 2007a. Mutation breeding of a spray chrysanthemum 'Argus' by gamma-ray irradiation and tissue culture. Flower Res. J. 15:52-57.
  21. Park, S.H., G.H. Kim, and B.R. Jeong. 2007b. Adventitious shoot regeneration from cultured petal explants of chrysanthemum. Hort. Environ. Biotechnol. 48:387-392.
  22. Petty, L.M., N.P. Harberd, I.A. Carré, B. Thomas, and S.D. Jackson. 2003. Expression of the Arabidopsis gai gene under its own promoter causes a reduction in plant height in chrysanthemum by attenuation of the gibberellin response. Plant Sci. 164:175-182. https://doi.org/10.1016/S0168-9452(02)00380-1
  23. Sauvadet, M.A., P. Brochard, and J. Boccon-Gibod. 1990. A protoplast-to-plant system in chrysanthemum: Differential responses among several commercial clones. Plant Cell Rep. 8:692-695. https://doi.org/10.1007/BF00269995
  24. Seiichi, F., J. de Jong, and W. Rademaker. 1995. Efficient genetic transformation of chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) using stem segments. Breed. Sci. 45:179-184.
  25. Song, J.Y., N.S. Mattson, and B.R. Jeong. 2011. Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium. Plant Cell Tiss Organ Cult. 107:295-304. https://doi.org/10.1007/s11240-011-9980-0
  26. Tanaka, K., Y. Kanno, S. Kudo, and M. Suzuki. 2000. Somatic embryogenesis and plant regeneration in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura). Plant Cell Rep. 19:946-953. https://doi.org/10.1007/s002990000225
  27. Tripepi, R.R. 1997. Adventitious shoot regeneration, p. 45-71. In: R.L. Geneve, J.E. Preece, and S.A. Merkle (eds.). Biotechnology of ornamental plants. CAB International, Wallingford, U.K.
  28. Waseem, K., M.S. Jilani, and M.S. Khan. 2009. Rapid plant regeneration of chrysanthemum (Chrysanthemum morifolium L.) through shoot tip culture. Afr. J. Biotechnol. 8:1871-1877.
  29. Yamaguchi, H., A. Shimizu, K. Degi, and T. Morishita. 2008. Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breed. Sci. 58:331-335. https://doi.org/10.1270/jsbbs.58.331
  30. Zalewska, M., J. Lema-Rumińska, and N. Miler. 2007. In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum. Sci. Hortic. 113:70-73. https://doi.org/10.1016/j.scienta.2007.01.019

Cited by

  1. Regeneration from chrysanthemum flowers: a review vol.37, pp.2, 2015, https://doi.org/10.1007/s11738-015-1773-3