Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Expression and Significance of Twist and E-cadherin in Ovarian Cancer Tissues

  • Wang, Wen-Shuang (Department of Gynecology, Yantai Yuhuangding Hospital) ;
  • Yu, Shou-Li (Department of Gynecology, Yantai Yuhuangding Hospital) ;
  • Yang, Xing-Sheng (Department of Gynecology, Qilu Hospial of Shandong University) ;
  • Chang, Shu-De (Department of Gynecology, Yantai Affiliated Hospital of Binzhou Medical College) ;
  • Hou, Jian-Qing (Department of Gynecology, Yantai Yuhuangding Hospital)
  • Published : 2013.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: To investigate the expression of Twist and E-cadherin in ovarian cancer tissues as well as the role of epithelial-mesenchymal transformation (EMT) in ovarian cancer metastasis. Method: The expressions of Twist and E-cadherin in 54 cases of ovarian cancer and paracancerous tissues were detected by Western blottin g and reverse transcriptase polymerase chain reaction. We used RNA interference to silence Twist expression in human ovarian cancer cell line, and detected E-cadherin expression using Western blotting. Results: There was an increase in the relative abundance of Twist proteins and a decrease in E-cadherin in ovarian cancer compared with normal ovary tissues (P < 0.05). The expression levels of Twist and E-cadherin mRNA were $1.49{\pm}0.53$ and $0.82{\pm}0.24$ in ovarian cancer, and $1.14{\pm}0.38$ and $1.08{\pm}0.19$ in paracancerous tissues, respectively. The difference between the indicators in ovarian cancer and in paracancerous tissues was statistically significant (P < 0.05). When the Twist expression was silenced in an ovarian cancer cell line, the expression of the E-cadherin protein increased (P<0.05). Conclusion: The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis.

Keywords

References

  1. Brasch J, Harrison OJ, Honig B, Shapiro L (2012). Thinking outside the cell: how cadherins drive adhesion. Trends Cell Biol, 22, 299-310. https://doi.org/10.1016/j.tcb.2012.03.004
  2. Cho EY, Choi Y, Chae SW, Sohn JH, Ahn GH (2006). Immunohistochemical study of the expression of adhesion molecules in ovarian serous neoplasms. Pathol Int, 56, 62-70. https://doi.org/10.1111/j.1440-1827.2006.01925.x
  3. Dara E, Scoazec JY, Walker-Combrouze F, et al (1997). Expression of cadherinsin benign, borderline, and malignant ovarian epithelial tumors: a clinicopathologic study of 60 cases. Hum Pathol, 28, 922-8. https://doi.org/10.1016/S0046-8177(97)90007-1
  4. De Beco S, Amblard F, Coscoy S (2012). New insights into the regulation of E-cadherin distribution by endocytosis. Int Rev Cell Mol Biol, 95, 63-108.
  5. De Wever O, Westbroek W, Verloes A, et al (2004). Critical role of N-cadherin in myofibroblast ivasion and migration in vitro stimulated by colon-cancer- cell-derived TGF-$\beta$or wounding. J Cell Sci, 117, 4691-703. https://doi.org/10.1242/jcs.01322
  6. Geho DH, Bandle RW, Clair T, Litta LA (2005). Physiological mechanisms of tumor-cell invasion and migration. Physiology, 20, 194-200. https://doi.org/10.1152/physiol.00009.2005
  7. Hosono S, Kajiyama H, Terauchi M, et al (2007). Expression of Twist in creases the risk for recurrence and for poor survival in epithelial ovarian carcinoma patients. Br J Cancer, 96, 314-20. https://doi.org/10.1038/sj.bjc.6603533
  8. Kajiyama H, Kikkawa F, Khin E, et al (2003). Dipeptidyl peptidase IV overexpression induces up-regulation of E-cadherin and tissue inhibitors of matrix metalloproteinases, resulting in decreased invasive potential in ovarian carcinoma cells. Cancer Res, 63, 2278-83.
  9. Kang Y, Massague J (2004). Epithelial-mesenchymal transitions: twist in development and metastasis. Cell, 118, 277-9. https://doi.org/10.1016/j.cell.2004.07.011
  10. Kim KJ, Jang SJ, Yu E (2008). Loss of E-cadherin and cytoplasmic-nuclear expression of b -catenin are the most useful immunoprofiles in the diagnosis of solid-pseudopapillary neoplasm of the pancreas. Hum Pathol, 39, 251-8. https://doi.org/10.1016/j.humpath.2007.06.014
  11. Kwok WK, Ling MT, Lee TW, et al (2005). Up-regulation of Twist in prostate cancer and its implication as a therapeutic target. Cancer Res, 65, 5153-62. https://doi.org/10.1158/0008-5472.CAN-04-3785
  12. Lander R, Nordin K, Labonne C (2011) . The F-box protein Ppa is a common regulator of core EMT factors Twist, Snail, Slug, and Sip1. J Cell Biol, 194, 17-25. https://doi.org/10.1083/jcb.201012085
  13. Larue L, Bellacosa A (2005). Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 3' kinase /AKT pathways. Oncogene, 24, 7443-54. https://doi.org/10.1038/sj.onc.1209091
  14. Lee JM, Dedhar S, Kalluri R, Thompson EW (2006). The epithelial-mesenchymal transition: new insights in signaling, development and disease. Cell Biol, 172, 973-81. https://doi.org/10.1083/jcb.200601018
  15. Lee TK, Poon RT, Yuen AP, et al (2006). Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition. Clin Cancer Res, 12, 5369-76. https://doi.org/10.1158/1078-0432.CCR-05-2722
  16. Myong NH (2012). Loss of E-cadherin and Acquisition of Vimentin in Epithelial-Mesenchymal Transition are Noble Indicators of Uterine Cervix Cancer Progression. Korean J Pathol, 46, 341-8. https://doi.org/10.4132/KoreanJPathol.2012.46.4.341
  17. Niu RF, Zhang L, Xi GM, et al (2007). Up-regulation of Twist induces angiogenesis and correlates with metastasis in hepatocellular carcinoma. J Exp Clin Cancer Res, 26, 385-94.
  18. Puisieux A (2009). Role of epithelial-mesenchymal transition in tumor progression. Bull Acad Natl Med, 193, 2017-34.
  19. Qin Q, Xu Y, He T, Qin C, Xu J ( 2012). Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms. Cell Res, 22, 90-106. https://doi.org/10.1038/cr.2011.144
  20. Radisky DC (2005). Epithelial-mesenchymal transition. J Cell Sci, 118, 4325-6. https://doi.org/10.1242/jcs.02552
  21. Thiery JP (2002). Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer, 2, 442-54. https://doi.org/10.1038/nrc822
  22. Thompson EW, Newgreen DF (2005). Carcinoma invasion and metastasis: a role for epithelial-mesenchymal transition? Cancer Res, 65, 5991-5.
  23. Yang J, Mani SA, Dongaher JL, et al (2004). Twist,a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell, 117, 927-39. https://doi.org/10.1016/j.cell.2004.06.006
  24. Yoshida J, Hofiuchi A, Kikuchi N, et al (2009). Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression. Med Mol Morphol, 42, 82-91. https://doi.org/10.1007/s00795-008-0436-5
  25. Zhang Z, Xie D, Li X, et al (2007). Significance of Twist expression and its association with E-cadherin in bladder cancer. Hum Pathol, 38, 598-606. https://doi.org/10.1016/j.humpath.2006.10.004

Cited by

  1. The Effect and Mechanism of CXCR4 Silencing on Metastasis Suppression of Human Glioma U87 Cell Line vol.296, pp.12, 2013, https://doi.org/10.1002/ar.22825
  2. Cascade vol.15, pp.22, 2014, https://doi.org/10.7314/APJCP.2014.15.22.9967
  3. Expression of Transcription Factor FOXC2 in Cervical Cancer and Effects of Silencing on Cervical Cancer Cell Proliferation vol.15, pp.4, 2014, https://doi.org/10.7314/APJCP.2014.15.4.1589
  4. BMI1 and TWIST1 Downregulated mRNA Expression in Basal Cell Carcinoma vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3797
  5. PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal-Transition in Non-small Cell Lung Cancer vol.290, pp.21, 2015, https://doi.org/10.1074/jbc.M114.636050
  6. Combined expression of ezrin and E-cadherin is associated with lymph node metastasis and poor prognosis in breast cancer vol.34, pp.1, 2015, https://doi.org/10.3892/or.2015.3967
  7. MiR-548c impairs migration and invasion of endometrial and ovarian cancer cells via downregulation of Twist vol.35, pp.1, 2016, https://doi.org/10.1186/s13046-016-0288-0
  8. Twist may be associated with invasion and metastasis of hypoxic NSCLC cells vol.37, pp.7, 2016, https://doi.org/10.1007/s13277-016-4896-2
  9. CXCL11 mediates TWIST1-induced angiogenesis in epithelial ovarian cancer vol.39, pp.5, 2017, https://doi.org/10.1177/1010428317706226
  10. inhibits epithelial-to-mesenchymal transition by targeting multiple pathways in triple-negative breast cancers pp.00219541, 2018, https://doi.org/10.1002/jcp.27222
  11. Combined Ezrin overexpression and E-cadherin downregulation as predictors of invasiveness, higher incidence of metastases, progression and poor prognosis in patients with cervical cancer vol.38, pp.1, 2018, https://doi.org/10.1097/01.XEJ.0000534715.52780.a4