The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Screening of Preventive Effects of Herbal Prescriptions in Rat Model of Arterial Thrombosis Induced by Ferric Chloride

  • Lee, Ki Mo (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Bang, Jihye (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Lee, In Sun (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Heo, Eun Jung (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Kim, Bu Yeo (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Kang, Hyung Won (Department of Neuropsychiatry, College of Oriental Medicine, WonKwang University) ;
  • Lyu, Yeoung Su (Department of Neuropsychiatry, College of Oriental Medicine, WonKwang University) ;
  • Jeon, Won Kyung (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
  • Received : 2013.04.30
  • Accepted : 2013.06.03
  • Published : 2013.06.30
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Abstract

Objectives: This study examined the effects of 8 types of herbal prescriptions prescribed to alleviate dementia symptoms in a rat model of arterial thrombosis induced by ferric chloride ($FeCl_3$). Methods: Thirty minutes before 35% $FeCl_3$ treatment, SD rats were intraperitoneally injected with the 8 types of herbal prescriptions, respectively. We tested the effects of the herbal prescriptions on time to occlusion (TTO) in an arterial thrombosis model using a laser Doppler flow meter. In addition, thrombus weight (TW) and collagen fiber damages were evaluated in the same condition. Results: Herbal prescriptions showed the following rank-order based on their TTO: Chong-myung-tang (CMT) > modified Jangwonhan 02 (LMK02) > Toki-shakuyaku-san (TSS) ${\geq}$ Oren-gedoku-to (OGT) ${\geq}$ Yokukansan (YKS). In particular, CMT (100 mg/kg, i.p.) and LMK02 (100 mg/kg, i.p.) delayed the TTO the most ($14.83{\pm}0.98$ and $13.67{\pm}1.03$ min, respectively) compared with the vehicle group ($7.95{\pm}0.78$ min, P<0.001). In addition, CMT, LMK02, and OGT treatment ($0.63{\pm}0.01$, $0.66{\pm}0.02$ and $0.67{\pm}0.01$ mg/mm, respectively) significantly reduced thrombus weight compared with the vehicle treatment ($0.78{\pm}0.03$, P<0.001) and also alleviated collagen fiber damage (CMT; $28.40{\pm}2.22$%, LMK02; $30.79 {\pm} 4.07$%, OGT; $26.20{\pm}1.48$%) in the vessels injured by $FeCl_3$. Therefore, CMT and LMK02 showed the greatest preventive activity in rat model of arterial thrombosis induced by $FeCl_3$. Conclusions: These results provide experimental evidence for traditional use of herbal prescriptions, suggesting that CMT and LMK02 extracts could be used to prevent vascular injury and thrombosis in the early stages of dementia.

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