Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Apoptotic Activity of Methanol Extract of Tongcao in HEp-2 Human Cervical Cancer Cells

  • Choi, Eun-Sun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Hang-Eun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University) ;
  • Cho, Sung-Dae (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University)
  • Received : 2012.12.11
  • Accepted : 2013.02.25
  • Published : 2013.03.31
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Abstract

Although tongcao has been reported to have anti-oxidative, anti-inflammatory and antipyretic effects, there is no report of the chemopreventive effect of tongcao in cancer cells. In the present study, we investigated the anti-proliferative activity of methanol extract of tongcao (MET) and its molecular target in HEp-2 human cervical cancer cells using MTS assay, western blot analysis, and DAPI staining. MET significantly decreases cell viability and induces apoptotic cell death. It affects Bid protein to be truncated resulting in the release of cytochrome c from mitochondria to cytosol whereas it did not affect other Bcl-2 family members. Thus, we clearly suggest that tongcao can be a potential naturally occurring plants having chemopreventive activity in cervical cancer.

비록 천연유래물질 중의 하나인 통초가 항산화작용, 항염증작용 및 해열작용과 같은 효능이 있다고 알려져있지만, 종양에서의 암예방효능에 대한 연구는 보고된 바가 없다. 본 연구에서는 사람 자궁경부암세포주인 HEP-2세포에서 통초메탄올추출물의 증식억제 효능 및 관련 분자표적을 확인하고자 하였다. 통초메탄올추출물은 세포증식을 유의성있게 억제하고, 세포사멸을 유도하는 것으로 나타났다. 또한, Bid의 발현에 영향을 주어 truncation을 유도하고, 이로 인해 cytochrome c가 마이토콘드리아에서 세포질로 이동하도록 유도하였지만, Bid 이외의 다른 Bcl-2 family에는 영향을 주지 못했다. 따라서, 이러한 결과를 종합해 볼 때, 통초메탄올추출물은 자궁경부암에서 암예방효능을 가진 잠재성있는 천연추출물이 될 수 있을 것으로 사료된다.

Keywords

References

  1. Munagala, R., Kausar, H., Munjal, C. and Gupta, R. C. Withaferin A induces p53-dependent apoptosis by repression of HPV oncogenes and upregulation of tumor suppressor proteins in human cervical cancer cells. Carcinogenesis, 32(11), 1697-705 (2011). https://doi.org/10.1093/carcin/bgr192
  2. Pisani, P., Bray, F. and Parkin, D. M. Estimates of the worldwide prevalence of cancer for 25 sites in the adult population. Int J Cancer, 97(1), 72-81 (2002). https://doi.org/10.1002/ijc.1571
  3. Mannhardt, B., Weinzimer, S. A., Wagner, M., Fiedler, M., Cohen, P., Jansen-Durr, P. and Zwerschke, W. Human papillomavirus type 16 E7 oncoprotein binds and inactivates growthinhibitory insulin-like growth factor binding protein 3. Mol Cell Biol, 20(17), 6483-95 (2000). https://doi.org/10.1128/MCB.20.17.6483-6495.2000
  4. Werness, B. A., Levine, A. J. and Howley, P. M. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science, 248(4951), 76-9 (1990). https://doi.org/10.1126/science.2157286
  5. Stoppler, H., Stoppler, M. C., Johnson, E., Simbulan-Rosenthal, C. M., Smulson, M. E., Iyer, S., Rosenthal, D. S. and Schlegel, R. The E7 protein of human papillomavirus type 16 sensitizes primary human keratinocytes to apoptosis. Oncogene, 17(10), 1207-14 (1998). https://doi.org/10.1038/sj.onc.1202053
  6. George W. S. and Derral R. H. "Atropical Garden Flora". Bishop Museum Press: Honolulu, HI, USA (2005).
  7. Shen, Y., Zeng, N., Liu, J., Jia, M., Zhang, Y., Wei, Y. and Ma, Y. Preliminary studies on pharmacological actions of tongca and xiao-tongcao polysaccharides. Zhongguo Zhong Yao Za Zhi, 23(12), 741-3, 765 (1998).
  8. Jia, M., Wei, Y., Ma, Y., Shen, Y. and Zhang, Y. Survey of botanical origin of tongcao (medulla Tetrapanacis) and identification of its commercial products. Zhongguo Zhong Yao Za Zhi, 22(8), 454-8, 510 (1997).
  9. Shen, Y., Zeng, N., Jia, M., Zhang, Y., Wei, Y. and Ma, Y. Experimental studies on a nti-inflammatory, antipyretic and diuretic effects of several species of tongcao and xiao-tongcao. Zhongguo Zhong Yao Za Zhi, 23(11), 687-90, 704-inside back cover (1998).
  10. Zeng, N., Shen, Y., Jia, M., Zhang, Y., Wei, Y. and Ma, Y. Experimental studies on antioxidative effect of tongcao and xiao-tongcao polysaccharides. Zhongguo Zhong Yao Za Zhi, 24(1), 46-8, inside back cover (1999).
  11. Girdhani, S., Bhosle, S. M., Thulsidas, S. A., Kumar, A. and Mishra, K. P. Potential of radiosensitizing agents in cancer chemo-radiotherapy. J Cancer Res Ther, 1(3), 129-31 (2005). https://doi.org/10.4103/0973-1482.19585
  12. Amin, A. R., Kucuk, O., Khuri, F. R. and Shin, D. M. Perspectives for cancer prevention with natural compounds. J Clin Oncol, 27(16), 2712-25 (2009). https://doi.org/10.1200/JCO.2008.20.6235
  13. Vidya Priyadarsini, R., Senthil Murugan, R., Maitreyi, S., Ramalingam, K., Karunagaran, D. and Nagini, S. The flavonoid quercetin induces cell cycle arrest and mitochondriamediated apoptosis in human cervical cancer (HeLa) cells through p53 induction and NF-kappaB inhibition. Eur J Pharmacol, 649(1-3), 84-91 (2010). https://doi.org/10.1016/j.ejphar.2010.09.020
  14. Kim, S. H., Lee, S. C., Song, Y. S. Involvement of both extrinsic and intrinsic apoptotic pathways in apoptosis induced by genistein in human cervical cancer cells. Ann N Y Acad Sci, 1171, 196-201 (2009). https://doi.org/10.1111/j.1749-6632.2009.04902.x
  15. Davids M.S., Letal A. Targeting the B-cell lymphoma/leukemia 2 family in cacner. J Clin Oncol, 30(25), 3127-3125 (2012). https://doi.org/10.1200/JCO.2011.37.0981
  16. Saito, M., Korsmeyer, S. J. and Schlesinger, P. H. BAX-dependent transport of cytochrome c reconstituted in pure liposomes. Nat Cell Biol, 2(8), 553-5 (2000). https://doi.org/10.1038/35019596