Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

Cytotoxicity and DNA Damage in Human Lung Cells Treated with Lanthanum Oxide and Neodymium Oxide

산화란타늄, 산화네오디뮴이 세포독성 및 DNA손상에 미치는 영향

  • Kim, Jong Kyu (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute) ;
  • Kim, Soo Jin (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute) ;
  • Kang, Min Gu (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute) ;
  • Chung, Young Hyun (Toxicity Research Team, Center for Chemicals Safety and Health, Occupational Safety & Health Research Institute)
  • 김종규 (산업안전보건연구원 화학물질센터 독성연구팀) ;
  • 김수진 (산업안전보건연구원 화학물질센터 독성연구팀) ;
  • 강민구 (산업안전보건연구원 화학물질센터 독성연구팀) ;
  • 정용현 (산업안전보건연구원 화학물질센터 독성연구팀)
  • Published : 2013.06.30
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Abstract

Objectives: The present study investigated cytotoxicity and DNA damage in human lung cells in vitro. Methods: Neodymium oxide and lanthanum oxide were dispersed by ultrasonic treatments. The assay was performed with MRC-5 (Human male fetus lung cell). Cytotoxicity and comet assay of lanthanum oxide and neodymium oxide were measured after 24 and 48 hours incubation. Results: After 24 hours of exposure to rare earth metals, the cytotoxicities of lanthanum oxide in more than $1{\mu}M$ concentration groups were significantly increased when compared to the control group, but the cytotoxicities of neodymiun oxide in more than $100{\mu}M$ concentration groups were statistically increased. After 48 hours exposure, cytotoxicities of both materials were statistically increased in $100,000{\mu}M$ concentration groups. Olive tail moments of the lanthanum oxide treated group were significantly increased when compared to the control group. Conclusions: The cytotoxicity of lanthanum oxide was higher than that of neodymium oxide. The DNA of MRC-5 cells treated with lanthanum oxide for 48 hours were significantly damaged.

Keywords

References

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