Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

2-Week repeated oral dose toxicity study of 1,4-dichlorobutane in rats

1,4-Dichlorobutane의 랫드 2주 반복경구투여독성시험

  • Kim, Jong-Kyu (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute) ;
  • Lee, In-Chul (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Hwan (College of Veterinary Medicine, Chonnam National University) ;
  • Baek, Hyung-Seon (College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Jin-Sook (Preclinical Research Center, ChemOn Inc.) ;
  • Song, Si-Whan (Preclinical Research Center, ChemOn Inc.) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Chung, Yong-Hyun (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
  • 김종규 (산업안전보건연구원 화학물질센터) ;
  • 이인철 (전남대학교 수의과대학) ;
  • 김성환 (전남대학교 수의과대학) ;
  • 백형선 (전남대학교 수의과대학) ;
  • 배진숙 ((주)켐온 전임상연구센터) ;
  • 송시환 ((주)켐온 전임상연구센터) ;
  • 김종춘 (전남대학교 수의과대학) ;
  • 정용현 (산업안전보건연구원 화학물질센터)
  • Published : 2013.03.31
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Abstract

Objectives: The present study investigated the potential subacute toxicity of 1,4-dichlorobutane (1,4-DCB) by a 2-week repeated oral dose in male Sprague-Dawley rats. Materials and Methods: The test chemical was administered once daily by gavage to male rats at dose levels of 0, 74, 222, 667, and 2000 mg/kg/day for 2 weeks. All rats were sacrificed at the end of treatment period. During the test period, clinical signs, mortality, body weights, food and water consumption, urinalysis, hematology, serum biochemistry, gross findings, and organ weights were examined. Results: At 2000 mg/kg/day, treatment-related clinical signs, as evidenced by hypothermia, decreased locomotor activity, piloerection, lying on side, and prone position were observed. All the rats were found dead on test day 2. At 667 mg/kg/day, polyuria, suppressed body weight gain, food consumption, and spleen and thymus weights, and increased adrenal gland and liver weights were observed.Hematological and serum biochemical investigations revealed increases in the alanine aminotransferase, alkaline phosphataseand total bilirubinand decreases in the serum $Na^+$ level, white blood cell count and lymphocyte ratio. There were no treatment-related adverse effects in the 74 and 222 mg/kg/day groups. Conclusions: In the present experimental conditions, target organs were determined to be spleen, thymus,and liver. The no-observed-adverse-effect level was considered to be 222 mg/kg/day in male rats.

Keywords

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