The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effects of Saengkankunbi-tang and Its Composition on Free Fatty Acid-Induced Lipotoxicity in HepG2 Cell

HepG2 cell에서 유리지방산 유발 지방독성에 대한 생간건비탕(生肝健脾湯)과 일부 조성 한약물의 효과

  • Hong, Sung-In (Dept. of East-West Integrated Medicine, Kyung-Hee University Medical Center)
  • 홍성인 (경희의료원 한방병원 동서협진실)
  • Published : 2013.03.30
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Abstract

Objectives : The aim of this study was to investigate whether the effects of extract from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba, Crataegi Fructus, Alismatis Rhizoma, Hoelen and Raphani Semen could protect HepG2 cells from palmitic acid-induced lipotoxicity through lysosomal and mitochondrial pathways in an in vitro model. Methods : To examine the effects of the extracts from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba, Crataegi Fructus, Alismatis Rhizoma, Hoelen and Raphani Semen on palmitic acid-induced lipotoxicity in HepG2 cells, we measured the contents of cell viability, cytotoxicity. Then to investigate the effects of the extract from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen, we measured that triglyceride, reactive oxygen species, ATP levels, glutathione levels, cytochrome c and cathepsin B. Results : The extracts from Saengkankunbi-tang and its composition had a cell-protective function. The extracts from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen controlled triglyceride over-accumulation in cells and reduced overproduction of reactive oxygen species. The extracts from Saengkankunbi-tang and Raphani Semen increased ATP and glutathione levels which had been decreased by lipotoxicity. The extracts from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen reduced leakage of cytochrome c and the extracts from Saengkankunbi-tang and Raphani Semen reduced leakage of cathepsin B in lipotoxicity. Conclusions : These results show that the extracts from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba and Raphani Semen have cell protective effects on palmitic acid-induced lipotoxicity through lysosomal and mitochondrial pathways.

Keywords

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