The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Jowiseungcheung-tang Extract on the Lipid Metabolism, Anti-oxidation and Inflammatory Reflex High Fat Diet Obese Rats

조위승청탕(調胃升淸湯) 추출액이 비만유도 쥐의 지질대사, 항산화계 및 염증반응계에 미치는 영향

  • Oh, Sung-Won (Dept. of Internal Medicine, Oriental Medical college, Sang-Ji University) ;
  • Kim, Byoung-Woo (Dept. of Internal Medicine, Oriental Medical college, Sang-Ji University)
  • 오성원 (상지대학교 부속한방병원 내과학교실) ;
  • 김병우 (상지대학교 부속한방병원 내과학교실)
  • Published : 2013.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of this study was to investigate the effects of Jowiseungcheung-tang (JWSCT) extract on the lipid metabolism, anti-oxidation and inflammatory reflex. Methods : Male Sprague-Dawley rats were fed a high fat diet for 8 weeks and were randomly divided into 4 groups (10 mice in each group): control group, 100 mg/kg JWSCT group, 200 mg/kg JWSCT group, 300 mg/kg JWSCT group. The control group was administered 100 mg/kg of water, but the other three groups were administered 100, 200, 300 mg/kg JWSCT extract for 4 weeks. After 4 weeks, we measured lipid level, thiobarbituric acid reactive substance (TBARS), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cytokines in plasma and liver. The gene expression level and the ratio of apo-B and apo-E were then investigated by way of reverse transcription -polymerase chain reaction (RT-PCR). Results : In the JWSCT group, compared with the control, free fatty acid, triglyceride, total cholesterol, LDL-cholesterol, TBARS, IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ decreased significantly in plasma and liver. However HDL-cholesterol, IL-10, GSH-Px, SOD and CAT increased. In the JWSCT group, compared with the control, the gene expression level and the ratio of apo-A and apo-E decreased significantly in the RT-PCR analysis. Conclusions : The extract of JWSCT has anti-obesity, anti-inflammatory and anti-oxidant effects.

Keywords

References

  1. 서영성, 이인규, 김대현. Proinflammatory Cytokines and Insulin Resistance in Nonobsese Women with High Body Fat and Low Fat Free Mass. Diabetes and Metabolism J 2007;31(2):136-43.
  2. 김종엽, 신현원, 정인경, 조성휘, 민선정, 이성진, 등. 비만도에 따른 Adiponectin, Leptin, Ghrelin 과 인슐린 저항성, 심혈관 위험인자의 상호 연관성. 대한내과학회지 2005;69(6):631-41.
  3. Ali SR, Holzer RG, Osterreicher CH, Takahashi H, Karin M. Dietary and Genetic Obesity Promote Liver Inflammation and Tumorigenesis by Enhancing IL-6 and TNF Expression. 대한 간암학회지 2010;10:82-3.
  4. 김달호, 이종형 편저. 注解補注黃帝內經素問 (上). 서울: 의성당; 2001, p. 652, 974.
  5. 진문개, 진가원. 高脂血症的中醫防治. 北京: 中 醫古籍出版社; 1988, p. 9-20.
  6. 허준. 東醫寶鑑. 서울: 법인문화사; 2002, p. 741,
  7. 서남준, 남동우, 이은옥, 심범상, 안규석, 김성훈. 가미태음조위탕 전탕액을 활용한 비만환자에 대한 임상연구. 동의생리병리학회지 2008;22(2) :446-52.
  8. 박선민, 안일성, 김다솔, 강선아, 권대영, 양혜정. 고지방식이로 비만을 유도한 암컷 백서에서 태음조위탕과 도담탕의 항비만 효과 및 기전. 한국응용생명화학회지 2010;53(1):44-50.
  9. 박태용, 신병철, 공재철, 송미영, 김은경, 서은아, 등. 체감의이인탕의 항비만 효과 연구. 동의생리병리학회지 2008;22(3):642-8.
  10. 송윤경, 임형호. 비만처방에서 마황의 임상활용에 대한 고찰: 용량, 효과, 부작용 등의 측면에서. 대한한방비만학회지 2007;7(1):1-7.
  11. 김성옥. 지방세포 3T3-L1에 인삼 사포닌 Re와 의이인 추출액 처리시 비만관련 유전자인 lipoprotein lipase, leptin 및 resistin 발현 조절에 미치는 영향. 생명과학회지 2007;17(11):1523-32.
  12. 김선민, 김경준. 창출 에탄올 추출물이 비만세포 매개 염증반응에 미치는 영향. 한방안이비인후피부과학회지 2011;24(1):45-63.
  13. Cho SW, Park SS. Effects of Taeyeumjoweetang on loss in bodyweight, plasma lipids and UCP1 revelation of fated white rats. J Korean Oriental Med 2004;25(2):87-97.
  14. Lee GJ, KimKY, Jeon BH. Effects of Taeyeumjowee -tang on the obesity of mouse and induced adipocyte 3T3-L1. Korean J Oriental Medical Pathology 1996;10(1):88-98.
  15. Jang YJ, Cho JH, Song BJ. Effect of Taeyeumjowee -tang and electroacupuncture combined-therapy on. J Korean Oriental Association for Study of Obesity 2001;1(1):77-83.
  16. 통계청. 2007년 국민건강통계결과. 서울: 보건 복지가족부; 2007, p. 56.
  17. 이기주, 김경효, 전병훈. 태음조위탕이 백서의 비만증 및 유도비만세포에 미치는 효과. 대한동의병리학회지 1996;10(1):88-98.
  18. 류재면, 황의완, 김종우, 지상은, 김은주, 박은혜, 등. 우울증모델 흰쥐에 대한 조위승청탕과 fluoxetine의 항우울효과 비교. 동의신경정신과학회지 2004;15(1):27-41.
  19. 한진수, 박성식. 조위승청탕의 위점막 손상 방어효과에 관한 연구. 사상체질의학회지 2002; 14(1):100-11.
  20. 류지걸. 생약 복합제재의 약효연구26: 조위승청탕의 항경련, 진통, 진경, 적출장관, 혈관, 혈압 및 호흡에 관한 영향. 생약학회지 1986;64:12-8.
  21. 이귀녕, 권오헌. 임상병리파일. 서울: 의학문화사; 2000, p. 148, 155-6, 164-5, 169-71, 182, 717.
  22. 비만관리를 위한 영양길잡이. 서울: 한미의학; 2003, p. 30-2.
  23. Nielsen S, Jensen MD. Obesity and cardivascular disease is body structure a factor. Curr Opin Lipidol 1997;8(4):200-4. https://doi.org/10.1097/00041433-199708000-00002
  24. Bruce AF, James D, Carpo MD. Biology of disease: Free radicals and tissue injury. Lab Invest 1982;47:412-26.
  25. Lioyd D. How to avoid oxygen. Science 1999;286:249. https://doi.org/10.1126/science.286.5438.249
  26. Kesavulu MM, Giri R, Kameswara Rao B, Apparao C. Lipid peroxidation and antioxidant enzyme levels in type 2 diabetics with microvascular complications. Diabetes Metab 2000;26(5):387-92.
  27. Oppenheim JJ. Cytokines: past, present and future. Int J of Hematology 2001;74(1):3-8. https://doi.org/10.1007/BF02982543
  28. Weigle DS. Appetite and the regulation of body composition. FASEB J 1994;8(3):302-10.
  29. Nielsen S, Jensen MD. Obesity and cardiovascular disease is body structure a factor. Curr Opin Lipidol 1997;8(4):200-4. https://doi.org/10.1097/00041433-199708000-00002
  30. Mohamed-Ali V, Goodrick S, Coppack SW. Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-${\alpha}$, in vivo. J Clin Endocrinol Metab 1997;82:4196-200.
  31. Mohamed-Ali V, Goodrick S, Coppack SW. Adipose tissue an as endocrine and paracrine organ. Int J Obes 1998;22:1145-58. https://doi.org/10.1038/sj.ijo.0800770
  32. 양정화. 조릿대 잎 추출물이 고지방식이 유도 비만마우스의(C57/BL6J) 염증성 사이토카인과 인슐린 저항성에 미치는 영향. 학위논문(석사). 전남대학교 대학원; 2008.
  33. Jansson JO, Walenius K, Wernstedt I, Ohlsson C, Dickson SL, Walenius V. On the site and mechanism of action of the anti-obesity effects of interleukin-6. Growth Horm IGF Res 2003; 13(A);28-32. https://doi.org/10.1016/S1096-6374(03)00051-0
  34. Pedersen BK, Steensberg A, Fischer C, Keller C, Ostrowski K, Schjerling P. Exercise and cytokines with particular focus on muscle-derived IL-6. Immunol Rev 2001;7:18-31.
  35. Marriott JB, Westby M, Cookson S, Guckian M, Goodbourn S, Muller G, et al. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-${\alpha}$ production. J Immunol 1998;161:4236-43.
  36. 민헌기. 임상내분비학. 서울: 고려의학; 1999, p. 667.
  37. Stiefel P, Montilla C, Muniz-Grijalvo O, Garcia -Lozano R, Alonso A, Miranda ML, et al. Apolipoprotein E gene polymorphism is related to metabolic abnormalities, but does not influence erythrocyte membrane lipid composition or sodiumlithium counter transport activity in essential hypertension. Metabolism 2001;50:157-60. https://doi.org/10.1053/meta.2001.19429
  38. Oh JY, Barrett-Connor E. Apolipoprotein E polymorphism and lipid levels differ by gender and family history of diabetes. the Rancho Bernardo Study. Clin Genet 2001;60:132-7.
  39. Hotamisligil GS. Adipose expression of tumor necrosis factor-${\alpha}$: direct role in obesity-linked insulin resistance. Science 1993;259:87-91. https://doi.org/10.1126/science.7678183