The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Single Oral Dose Toxicity Test of Persicae Semen Aqueous Extracts in Mice

  • Cho, Hun-Bum (Department of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Ji-Ha (Department of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Seo, Bu-Il (Department of Herbology, College of Oriental Medicine, Daegu Haany University) ;
  • Cho, Su-Yeon (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Kyu-Ryul (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Choi, Seung-Hoon (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Han, Chang-Kyun (Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University) ;
  • Song, Chang-Hyun (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Soo-Jin (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
  • Received : 2013.04.29
  • Accepted : 2013.05.23
  • Published : 2013.05.30
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Abstract

Objectives : This study was to evaluate the single dose toxicity of Persicae Semen (PS) in ICR mice. Methods : Aqueous extracts of PS (Yield = 18.60%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines (2009-116, 2009). Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined. Results : Amygdalin contents in PS aqueous extracts were detected as $32.50{\pm}5.96{\mu}g/ml$. We could not find any PS extracts treatment related mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations up to 2,000 mg/kg in both female and male mice, except for transient and slight loss of locomotion detected in female and male mice treated with 2,000 mg/kg. In addition, pharmacological immunomodulatory effects related findings were also demonstrated in 2,000mg/kg treated female and male mice as hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes. Conclusions : Based on the results of this experiment, the approximate lethal dose (ALD) of PS extracts after single oral treatment in female and male mice were considered above 2,000 mg/kg, respectively. It should be carefully used in clinics because the possibilities of respiratory or neurological disorders were observed when administered over 2,000 mg/kg of PS extract related to amygdalin.

Keywords

References

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