Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Assessment of Skin Toxicity Using Skin Equivalents Containing Cervi cornus Colla

녹각교 함유 인공피부를 이용한 피부독성도의 검사

  • Kim, Jandi (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Li, Hailan (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Jeong, Hyo-Soon (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Yun, Hye-Young (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Baek, Kwang Jin (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Kwon, Nyoun Soo (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Choi, Hye-Ryung (Department of Dermatology, Seoul National University College of Medicine) ;
  • Park, Kyoung-Chan (Department of Dermatology, Seoul National University College of Medicine) ;
  • Kim, Dong-Seok (Department of Biochemistry, Chung-Ang University College of Medicine)
  • 김잔디 (중앙대학교 의과대학 생화학교실) ;
  • 이해란 (중앙대학교 의과대학 생화학교실) ;
  • 정효순 (중앙대학교 의과대학 생화학교실) ;
  • 윤혜영 (중앙대학교 의과대학 생화학교실) ;
  • 백광진 (중앙대학교 의과대학 생화학교실) ;
  • 권년수 (중앙대학교 의과대학 생화학교실) ;
  • 최혜령 (서울대학교 의과대학 피부과학교실) ;
  • 박경찬 (서울대학교 의과대학 피부과학교실) ;
  • 김동석 (중앙대학교 의과대학 생화학교실)
  • Received : 2012.08.13
  • Accepted : 2013.01.15
  • Published : 2013.03.31
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Abstract

To substitute animal test, skin equivalents (SEs) have been developed for skin irritation and corrosion test. Recently, we have developed new SEs containing Cervi cornus Colla (CCC). In the present study, we used the SEs for cutaneous cytotoxicity test. Sodium dodecylsulfate (SDS) or sodium carbonate was applied to the SEs-, and the epidermal damage by H&E and immunohistochemical stains was evaluated. Our results showed that SDS or sodium carbonate affected the epidermal part of SEs containing CCC in a dose-dependent manner and decreased the expression of p63. It is concluded that SEs containing CCC could be used for an alternative model of animal test and would be greatly helpful in the development of in vitro irritation and corrosion test.

피부 자극과 부식 검사에 사용되는 동물실험을 대체하기 위한 방법으로 인공피부가 개발되어 왔다. 최근에 본 연구진은 녹각교를 함유하는 새로운 인공피부를 구축하였다. 현재 연구에서는 녹각교를 포함하는 인공피부를 사용하여 물질의 독성도 검사를 수행하였다. 그리하여 sodium dodecylsulfate (SDS) 또는 sodium carbonate를 인공피부에 도포하였고 표피의 손상 정도를 H&E와 면역조직화학 염색을 통하여 평가하였다. 인공피부의 표피는 SDS와 sodium carbonate에 의해 농도 의존적으로 영향을 받았다. 더 나아가 이들 물질에 의하여 p63의 발현이 감소하였다. 그러므로 녹각교를 함유하는 인공피부는 동물실험을 대체할 수 있는 모델로써 사용될 수 있고 in vitro에서 자극과 부식 검사 시험법의 발전에 도움을 줄 것으로 예상된다.

Keywords

References

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