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버찌(Prunus yedoensis) 추출물의 항산화 활성 평가 및 돈육 패티에 이용

Antioxidant Activities of Water or Methanol Extract from Cherry (Prunus yedoensis) and Its Utilization to the Pork Patties

  • 최필수 (전남대학교 동물자원학부) ;
  • 김형상 (전남대학교 동물자원학부) ;
  • 진구복 (전남대학교 동물자원학부)
  • Choi, Pil Soo (Department of Animal Science, Chonnam National University) ;
  • Kim, Hyeong Sang (Department of Animal Science, Chonnam National University) ;
  • Chin, Koo Bok (Department of Animal Science, Chonnam National University)
  • 투고 : 2013.01.11
  • 심사 : 2013.03.23
  • 발행 : 2013.04.30

초록

본 연구는 버찌를 메탄올과 물로 추출한 후 항산화 활성을 측정하여, 버찌의 메탄올과 물 추출물을 돈육패티에 첨가하여 육제품의 천연항산화제의 가능성을 알아보기 위하여 본 연구를 수행하였다. 버찌의 메탄올 추출물과 물 추출물의 총 페놀성 화합물 함량은 버찌 메탄올 추출물이2.17 g/100 g, 물 추출물이 2.77 g/100 g값을 나타내었다. 1,1-Diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity에서 버찌 메탄올 추출물이 대조구인 ascorbic acid와 농도별로 차이를 보이지 않았고, 2% 농도에서 대조구와 처리구간에 유사한 DPPH radical scavenging activity를 보였다. 환원력은 L-ascorbic acid에 비하여 낮은 수치를 보였으며 추출용매에 따른 환원력의 차이는 미미하였다. 철이온 흡착력은 버찌 메탄올 추출물이 19.8-94.0%, 버찌 물 추출물은 17.8-93.5%를 나타내었다. 버찌 메탄올 추출물은 0.5%의 농도까지 증가하다가 최종 농도인 2%까지 유의적인 차이를 보이지 않았고, 물 추출물은 농도가 증가할수록 철이온 흡착력이 증가하는 경향을 보였다. 특히 낮은 농도에서는 차이를 보였지만 농도가 증가할수록 대조구와 유사한 수치를 보였다. 버찌의 메탄올 추출물을 각각 0.5%, 1.0% 첨가한 돈육 패티에서 pH와 색도(명도, 적색도, 황색도)가 감소하였고, 지방산패도를 측정하기 위해 수행한 thiobarbituric acid reactive substances(TBARS) 결과에서 malondialdehyde(MDA) 함량이 대조구와 butylated hydroxytoluene(BHT)를 첨가한 참조구에 비해 낮은 결과를 나타내었으나, 저장 중 총균수나 대장균군수에는 효과가 없는 것으로 나타났다. 이러한 결과를 종합하면 버찌 추출물이 항산화 효과를 보여 천연 항산화제로써의 기능성 육제품 개발에 이용 가능할 것으로 평가된다. 하지만 버찌 추출물을 첨가하면 색도를 저하시키므로 이에 대한 보완 연구가 필요할 것으로 판단된다.

This study was performed to investigate the antioxidant activity of cherry added into meat products. Water and methanol were used to extract the antioxidant compounds from cherry. Total phenolic compounds of the methanol and water extract of cherry were 2.17 g/100 g and 2.77 g/100 g, respectively. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of methanol extract showed similar activities to those with ascorbic acid at all concentrations (from 0.1% to 2.0%). Especially, water extract of cherry showed similar activity to those of ascorbic acid (AA), and methanol extract, when 2% of cherry extract was added. The reducing power of cherry was not comparable to those with AA, however no differences in reducing power were observed between the water and methanol extract. The iron chelating ability of cherry was observed in the range of 17.8-94.0% at both water and methanol extracts. An increased iron chelating ability was observed with increased concentration up to 2%. Iron chelating ability for water extract of cherry tended to be lower than those with methanol extract. After pork patties were manufactured with methanol extract of cherry at 0.5 and 1.0%, physicochemical properties, lipid oxidation and microbial changes of patties were measured. The addition of methanol extract of cherry reduced pH, brightness, redness, yellowness and thiobarbituric acid reactive substance (TBARS). During 14 d of storage, pH, TBARS and microbial counts were increased, while redness and yellowness values were decreased. Since the addition of methanol extract of cherry lowered TBARS during storage, it could be used as a natural antioxidant in meat products.

키워드

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