Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

  • Kim, Young Jo (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety) ;
  • Song, Bo Ra (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety) ;
  • Lim, Jong Su (College of Veterinary Medicine, Konkuk University) ;
  • Heo, Eun Jeong (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety) ;
  • Park, Hyun Jung (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety) ;
  • Wee, Sung Hwan (Veterinary Pharmaceutical Management Division, Animal and Plant Quarantine Agency) ;
  • Oh, Soon Min (Quarantine Policy Division, Ministry of Agriculture, Food and Rural Affairs) ;
  • Moon, Jin San (Veterinary Pharmaceutical Management Division, Animal and Plant Quarantine Agency)
  • Received : 2013.01.04
  • Accepted : 2013.04.09
  • Published : 2013.04.30
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Abstract

Salmonella Enteritidis is responsible for causing foodborne diseases upon consumption of egg products. While cases of S. Enteritidis isolation from eggs have been reported in other countries, no such cases have previously been reported in Korea. In this study, we report the first isolation and identification of S. Enteritidis from domestically distributed eggs in Korea. Eggs were collected from eight countrywide grocery stores during different seasons between 2011 and 2012. Egg contents and washing solution of egg shells were incubated in buffered peptone water, and the enriched broth was further enriched in tetrathionate broth and Rappaport-Vassiliadis. The secondary enriched broth was streaked on xylose lysine desoxycholate agar. The suspected colonies were confirmed to S. Enteritidis by a biochemical test, serotyping, and PCR test. Genetic relatedness among the isolates was analyzed using Diversilab Salmonella kit. Three strains of S. Enteritidis were isolated from egg contents and egg shells collected from grocery stores of the Eumseong-city in the fall of 2011. All three stains showed resistance to chloramphenicol, streptomycin, nalidixic acid, and ampicillin by the disk diffusion method. In addition, the isolates showed more than 99% DNA homology, indicating that they were presumably identical strains. Therefore, there is a requirement to monitor and control against S. Enteritidis from eggs in Korea.

Keywords

References

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