Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Different Clinical Outcomes of Entamoeba histolytica in Malaysia: Does Genetic Diversity Exist?

  • Anuar, Tengku Shahrul (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia) ;
  • Al-Mekhlafi, Hesham M. (Department of Parasitology, Faculty of Medicine, University of Malaya) ;
  • Abdul Ghani, Mohamed Kamel (Programme of Biomedical Sciences, School of Diagnostic and Applied Health Sciences, Universiti Kebangsaan Malaysia) ;
  • Azreen, Siti Nor (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia) ;
  • Salleh, Fatmah Md. (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia) ;
  • Ghazali, Nuraffini (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia) ;
  • Bernadus, Mekadina (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia) ;
  • Moktar, Norhayati (Department of Parasitology & Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia)
  • Received : 2012.11.13
  • Accepted : 2013.02.10
  • Published : 2013.04.30
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Abstract

The present study was conducted to investigate the clinical outcomes of Entamoeba histolytica infection in symptomatic and asymptomatic Orang Asli (aborigine) communities in Malaysia. Examination was performed on 500 stool samples obtained from Orang Asli communities in 3 different states using formalin-ether concentration, trichrome staining, and single-round PCR techniques. Out of 500 stool samples, single infection of E. histolytica, Entamoeba dispar, and Entamoeba moshkovskii was identified in 3.2%, 13.4%, and 1%, respectively. In addition, 10 samples had mixed infections with E. histolytica and E. dispar. Six samples containing E. dispar were also positive for E. moshkovskii, and only 2 samples had E. histolytica in association with E. dispar and E. moshkovskii. Seventeen E. histolytica-positive samples were from symptomatic subjects, whereas the remaining 11 samples came from asymptomatic subjects. These findings suggest a predominant distribution of pathogenic potential of E. histolytica strains in this community. Therefore, further studies on genotyping of E. histolytica is required, to find out association between E. histolytica genotype and the outcome of the infection.

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