DOI QR코드

DOI QR Code

Heavy Metal and Amino Acid Contents of Soybean by Application of Sewage and Industrial Sludge

생활하수 및 산업폐수 슬러지 처리에 따른 콩의 중금속 및 아미노산 함량

  • Moon, Kwang-Hyun (Dept. of Health and Longevity, Sunchang County office) ;
  • Kim, Jae-Young (Division of Food Chemical Residues, Dept. Food Safety Evaluation, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Adminstration) ;
  • Chang, Moon-Ik (Division of Food Chemical Residues, Dept. Food Safety Evaluation, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Adminstration) ;
  • Kim, Un-Sung (Quality Improvement Evaluation Team, Korea Health Industry Development Institute) ;
  • Kim, Seong-Jo (Dept. of Bio-Environmental Chemistry, Wonkwang University) ;
  • Baek, Seung-Hwa (Dept. of Biofood Science and Biotechnology, Chungbuk Provincial University)
  • 문광현 (순창군청 건강장수과) ;
  • 김재영 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 화학물질과) ;
  • 장문익 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 화학물질과) ;
  • 김운성 (한국보건산업진흥원 품질향상평가팀) ;
  • 김성조 (원광대학교 생물환경화학과) ;
  • 백승화 (충북도립대학교 바이오식품생명과학과)
  • Received : 2012.08.03
  • Accepted : 2012.10.17
  • Published : 2013.02.28

Abstract

This study investigates the effects of accumulated levels of heavy metals and nutrients of cultivated soybean plant tissues, after the continuous application of sewage sludge (SS) and industrial sludge (IS). SS and IS were applied to soybean plants at loading of 0, 11.25, 22.50, and 45.00 Mg/ha, and the contents of heavy metals (Cd, Pb, Ni, Cu, and Zn), proteins, and amino acids in the cultivated soybean plants were measured. The Cd content in the soybean was 0.02~0.05 mg/kg, which is within the safety level set in the standard, and that of Pb was 0.02~0.15 mg/kg, which is also within the safety level except for IS 45 Mg/ha. The soybean harvest quantity was higher in the treatment groups than the control group in the first year. However, in the second year, SS had lower harvest and IS had the same level or a decreasing tendency, compared with the control group. In the first year, the content of amino acid which followed handling of SS was increased in the sludge groups more than in the control group in the case of glutamate. However, the influence of continuous application was increased in the sludge groups in the case of amino acids of 12 types. In conclusions, the accumulation in soybean of heavy metals by sludge treatment is not a problem, but the decreased yields needs to be considered. In addition, the most appropriate level of sludge treatment was 11.25 Mg/ha.

본 연구는 생활하수 및 산업폐수 슬러지를 연차적으로 경작지에 처리한 후, 재배된 콩 식물체의 중금속 흡수 정도와 영양성분에 미치는 영향을 조사하였다. 처리량은 무처리, 11.25, 22.50 및 45.00 Mg/ha로 하였으며, 재배된 콩 식물체의 부위별 Cd, Pb, Ni, Cu 및 Zn 함량과 콩 종실의 수확량, 단백질 및 아미노산 함량을 조사하였다. 콩 종실 중의 Cd 함량은 0.02~0.05 mg/kg으로 기준치에 안전한 수준이었고, Pb 함량은 산업폐수 슬러지 45 Mg/ha를 제외하고 0.02~0.15 mg/kg으로 기준치에 적합하였다. 또한, 모든 중금속 축적률은 잎 또는 뿌리보다 식용부위인 콩 종실에서 상대적으로 낮았고, 슬러지 처리량의 증가에도 함량 변화는 적어 식용으로서 안전함을 확인하였다. 종실의 수확량은 1년차에 슬러지 처리군이 무처리군보다 높았지만 2년차에는 생활하수 슬러지의 경우 낮은 수확량을 보였고, 산업폐수 슬러지의 경우 유사한 수준 또는 감소하는 경향이었다. 생활하수 슬러지 처리에 따른 아미노산 함량은 glutamate 경우에만 슬러지 처리군이 무처리군보다 증가하는 경향이었다. 하지만 연용에 따른 변화는 12종에서만 무처리군보다 증가하고 있었다. 산업폐수 슬러지는 1년차의 경우 15종이 무처리군에서 가장 높았고, 2년차에서는 11.25 Mg/ha에서 17종 모두가 가장 높은 결과를 나타내었다. 결론적으로 콩 종실 중 중금속 축적량은 문제가 되지 않았으나 수량이 감소되는 점을 감안할 때 가장 적합한 슬러지 처리 수준은 11.25 Mg/ha임을 확인하였다.

Keywords

References

  1. ME. 2008. 2007 Statistics of sewerage. Ministry of Environment, Gwacheon, Korea. p 12.
  2. ME. 2011. 2010 Statistics of sewerage. Ministry of Environment, Gwacheon, Korea. p 14-15.
  3. Kim KS. 2007. The present state and improvement of sludge disposal from sewage treatment plants. J KSEE 29: 8-16.
  4. Park YJ, Shin WS, Choi SJ, Lee HH. 2010. Solidification/ stabilization of heavy metals in sewage sludge prior to use as a landfill cover material. J KSEE 32: 665-675.
  5. Choi IS, Jung HS, Han IS. 2009. Study on sludge reduction by sludge solubilization and change of operation conditions of sewage treatment process. J KSEE 31: 1113-1122.
  6. Lee JC, Kim JH, Choi KK, Park DW. 2007. Biological hydrogen production from mixed organic waste of food and activated sludge by pre-treatment. J KSEE 29: 1044-1050.
  7. Kim SJ, Baek SH, Chung DJ. 1992. Effects of municipal sewage sludge on contents of lead and copper in crop plants. Korean J Environ Agric 11: 9-19.
  8. Choi J, Lee DH, Park M, Choi CR, Kim KS. 2002. Effect of municipal sewage sludge on soil chemical properties and growth of rose (Rosa hybrida L.). Korean J Envrion Agric 21: 117-121. https://doi.org/10.5338/KJEA.2002.21.2.117
  9. NIAST. 2000. Method of soil and plant analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea. p 102-147.
  10. Cao HF, Chang AC, Page AL. 1984. Heavy metal contents of sludge-treated soils as determined by three extraction procedure. J Environ Qual 13: 632-634.
  11. Gange TJ, Page AL. 1974. Rapid acid dissolution of plant tissue for cadmium determination by atomic absorption of spectrophotometry. At Absorpt Newsl 13: 131-134.
  12. AOAC. 1995. Official method of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 69-74.
  13. Waters. 1993. AccQ-Tag amino acid analysis system: Operator's manual. Waters, Philadelphia, PA, USA.
  14. FAO/WHO. 2011. Evaluation of certain food additives and contaminants. FAO/WHO, Geneva, Switzerland. p 149-163.
  15. Won KP, Kim NK, So YS, Jung SY, Yoon HK, Kim HD, Chang MI. 1996. The annual report of Korea food & drug administration: A study on the trace minerals of food. The Annual Report of KFDA. Vol 1, p 58-70.
  16. Kim HY, Kim JI, Kim JC, Park JE, Lee KJ, Kim SI, Oh JH, Jang YM. 2009. Survey of heavy metal contents of circulating agricultural products in Korea. Korean J Food Sci Technol 41: 238-244.
  17. KFDA. 2011. Food code. Korea Food and Drug Administration, Cheongwon, Korea. p 2-1-9.
  18. KFDA. 2012. Codex standard inflection research. Available from: http://foodnara.go.kr/codex/index.do?nMenuCode=15&gb=pollutant&page=&hazard_seq=6&includeUrl=/codex/hview.jsp&type=2#codex
  19. European Union. 2006. Commission regulation (EC) No 1881/2006 of 19 December 2006: setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union L364: 20-22.
  20. KFDA. 2012. Codex standard inflection research. Available from: http://foodnara.go.kr/codex/index.do?nMenuCode=15&gb=pollutant&page=&hazard_seq=14&includeUrl=/codex/hview.jsp&type=2#codex
  21. Song HI, Yu TS. 1991. Morphological changes of Hansenula anomala B-7 by cadmium ion. Kor J Microbiol 29: 397-401.
  22. Kim KS, Kim MJ, Lee KA, Kwon DY. 2003. Physico-chemical properties of Korean traditional soybeans. Korean J Food Sci Technol 35: 335-341.
  23. Wei CH, Sok DE, Yang YH, Oh SH, Kim HC, Yoon WK, Kim HM, Kim MR. 2006. Protein composition of domestic and glyphosate-tolerant soybean. J Korean Soc Food Sci Nutr 35: 470-475. https://doi.org/10.3746/jkfn.2006.35.4.470
  24. Moon HK, Lee SW, Moon JN, Kim DH, Yoon WJ, Kim GY. 2011. Quality characteristics of various beans in distribution. J East Asian Soc Dietary Life 21: 215-221.
  25. Kim SL, Chi HY, Son JR, Park NK, Ryu SN. 2005. Physicochemical characteristics of soybean seed coat and their relationship to seed lustre. Korean J Crop Sci 50: 123-131.
  26. Kim DM, Jin JS, Kim KH. 1990. Morphological characteristics and proximate compositions of the recommended soybean varieties in Korea. Korean J Food Sci Technol 22: 398-404.
  27. Läuchli A, Pfluger R. 1980. Potassium transport through plant cell membranes and metabolic role of potassium in plants. IPI Res Top 6: 5-57.
  28. Preusser E, Khalil FA, Goring H. 1981. Regulation of activity of the granule-bound starch synthetase by monovalent cations. Biochem Physiol Pflanz 176: 744-752. https://doi.org/10.1016/S0015-3796(81)80059-5
  29. Trevors JT, Stratton GW, Gadd GM. 1986. Cadmium transport, resistance, and toxicity in bacteria, algae, and fungi. Can J Microbiol 32: 447-464. https://doi.org/10.1139/m86-085

Cited by

  1. Soybean cadmium concentration: validation of a QTL affecting seed cadmium accumulation for improved food safety vol.203, pp.1, 2015, https://doi.org/10.1007/s10681-014-1297-8