Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
권호 표지

Vasorelaxant effect of Salvia miltiorrhiza Radix extract on isolated rat aorta

단삼(丹蔘) 추출물의 흰쥐 흉부 대동맥 이완 효과

  • Received : 2012.11.29
  • Accepted : 2012.12.06
  • Published : 2013.02.20
Download PDF
⟨ Previous Next ⟩

Abstract

단삼(丹蔘) (Salvia miltiorrhiza)은 꿀풀과(일과(―科) Lamiaceae)에 속하는 다년생 초본으로, 중국이 원산지이며 약용하기 위해 우리나라에서도 널리 재배하고 있다. 신농본초경(神農本草經) 상품(上品)에 수재(收載)되어 있으며, 거어지통(祛瘀止痛), 양혈소종(凉血消腫), 청심제번(淸心除煩), 활혈조혈(活血調血) 등의 효능이 있어 부인과 질환에 많이 사용되고 있는 약재에 속한다. 본 연구에서는 단삼(丹蔘) 추출물이 흰쥐의 흉부 대동맥 절편에 어떠한 양상으로 작용하는지 확인하고자 하였으며, 그 결과 단삼 추출물 특히 헥산 분획에서 강력한 혈관 이완 작용이 나타났으며, 혈관 내피 세포의 존재 유무에 상관없이 농도 의존적으로 혈관을 이완시켰으나 혈관 내피 세포가 존재하는 상황에서 더욱 강력한 혈관 이완 작용을 보였다. 이러한 과정에 NO에 의한 cGMP 증가가 주요하게 작용하는 것으로 추정되었으며, 칼슘 통로 차단 효과에 의한 세포 내 $Ca^{2+}$의 감소도 관여하는 것으로 생각된다.

Keywords

References

  1. Ji XY, Tan BK, Zhu YZ. Salvia miltiorrhiza and ischemic diseases. Acta Pharmacol. Sin. 2000;21:1089- 1094.
  2. Fung KP, Zeng LH, Wu J, Wong HN, Lee CM, Hon PM, Chang HM, Wu TW. Demonstration of the myocardial salvage effect of lithospermic acid B isolated from the aqueous extract of Salvia miltiorrhiza. Life Sci. 1993; 52:239-244.
  3. Kuang P, Tao Y, Tian Y. Radix Salviae miltiorrhizae treatment results in decreased lipid peroxidation in reperfusion injury. J. Tradit. Chin. Med. 1996;16:138-142.
  4. Wu TW, Zeng LH, Fung KP, Wu J, Pang H, Grey AA, Weisel RD, Wang JY. Effect of sodium tanshinone IIA sulfonate in the rabbit myocardium and on human cardiomyocytes and vascular endothelial cells. Biochem. Pharmacol. 1993;46:2327-2332. https://doi.org/10.1016/0006-2952(93)90624-6
  5. Zhou W, Ruigrok TJ. Protective effect of danshen during myocardial ischemia and reperfusion: an isolated rat heart study. Am. J. Chin. Med. 1990;18:19-24 https://doi.org/10.1142/S0192415X90000046
  6. Tang MK, Ren DC, Zhang JT, Du GH. Effect of salvianolic acids from Radix Salviae miltiorrhizae on regional cerebral blood flow and platelet aggregation in rats. Phytomedicine. 2002;9:405-409. https://doi.org/10.1078/09447110260571634
  7. Ji X, Tan BK, Zhu YC, Linz W, Zhu YZ. Comparison of cardioprotective effects using ramipril and danshen for the treatment of acute myocardial infarction in rats. Life Sci. 2003; 73:1413-1426. https://doi.org/10.1016/S0024-3205(03)00432-6
  8. Cao CM, Xia Q, Zhang X, Xu WH, Jiang HD, Chen JZ. Salvia miltiorrhiza attenuates the changes in contraction and intracellular calcium induced by anoxia and reoxygenation in rat cardiomyocytes. Life Sci. 2003;72:2451 -2463. https://doi.org/10.1016/S0024-3205(03)00142-5
  9. Chen KJ. Certain progress in the treatment of coronary heart disease with traditional medicinal plants in China. Am. J. Chin. Med. 1981;9:193-196 https://doi.org/10.1142/S0192415X81000251
  10. Kamata K, Iizuka T, Nagai M, Kasuya Y. Endothelium-dependent vasodilator effects of the extract from Salviae miltiorrhizae radix. A study on the identification of lithospermic acid B in the extracts. Gen. Pharmacol. 1993;24:977-981. https://doi.org/10.1016/0306-3623(93)90176-X
  11. Lei XL, Chiou GC. Studies on cardiovascular actions of Salvia miltiorrhiza. Am. J. Chin. Med. 1986 ;14:26-32. https://doi.org/10.1142/S0192415X86000053
  12. Li CP, Yung KH, Chiu KW. Hypotensive action of Salvia miltiorrhiza cell culture extract. Am. J. Chin. Med. 1990;18:157 -166. https://doi.org/10.1142/S0192415X90000204
  13. Cheng YY, Fong SM, Chang HM. Protective action of Salvia miltiorrhiza aqueous extract on chemically induced acute myocardial ischemia in rats. Zhong Xi Yi Jie He Za Zhi. 1990;10:582, 609-611.
  14. Chan K, Chui SH, Wong DY, Ha WY, Chan CL, Wong RN. Protective effects of danshensu from the aqueous extract of Salvia miltiorrhiza (danshen) against homocysteine-induced endothelial dysfunction. Life Sci. 2004;75:3157-3171. https://doi.org/10.1016/j.lfs.2004.06.010
  15. Kohda H, Takeda O, Tanaka S, Yamasaki K, Yamashita A, Kurokawa T, Ishibashi S. Isolation of inhibitors of adenylate cyclase from danshen, the root of Salvia miltiorrhiza. Chem. Pharm. Bull. (Tokyo). 1989;37:1287- 1290. https://doi.org/10.1248/cpb.37.1287
  16. Liu GT, Zhang TM, Wang BE, Wang YW. Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes. Biochem. Pharmacol. 1992;43:147-152. https://doi.org/10.1016/0006-2952(92)90271-J
  17. Lin YL, Wu CH, Luo MH, Huang YJ, Wang CN, Shiao MS, Huang YT. In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells. J. Ethnopharmacol. 2006;105:215-222 https://doi.org/10.1016/j.jep.2005.10.021
  18. Lay IS, Chiu JH, Shiao MS, Lui WY, Wu CW. Crude extract of Salvia miltiorrhiza and salvianolic acid B enhance in vitro angiogenesis in murine SVR endothelial cell line. Planta Med. 2003;69:26-32. https://doi.org/10.1055/s-2003-37034
  19. Lay IS, Hsieh CC, Chiu JH, Shiao MS, Lui WY, Wu CW. Salvianolic acid B enhances in vitro angiogenesis and improves skin flap survival in Sprague-Dawley rats. J. Surg. Res. 2003;115:279-285. https://doi.org/10.1016/S0022-4804(03)00226-9
  20. Chen YH, Du GH, Zhang JT. Salvianolic acid B protects brain against injuries caused by ischemia-Nreperfusion in rats. Acta Pharmacol. Sin. 2000;21:463 -466.
  21. Zhao BL, Jiang W, Zhao Y, Hou JW, Xin WJ. Scavenging effects of Salvia miltiorrhiza on free radicals and its protection for myocardial mitochondrial membranes from ischemia-Rreperfusion injury. Biochem. Mol. Biol. Int. 1996;38:1171-1182.
  22. Martin PM, Horwitz KB, Ryan DS, McGuire WL. Phytoestrogen interaction with estrogen receptors in human breast cancer cells. Endocrinology 1978;103:1860-1867. https://doi.org/10.1210/endo-103-5-1860
  23. Miksicek RJ. Commonly occurring plant flavonoids have estrogenic activity. Mol. Pharmacol. 1993;44:37-43.
  24. Wang TT, Sathyamoorthy N, Phang JM. Molecular effects of genistein on estrogen receptor mediated pathways. Carcinogenesis. 1996;17:271-275. https://doi.org/10.1093/carcin/17.2.271
  25. Mishra SK, Abbot SE, Choudhury Z, Cheng M, Khatab N, Maycock NJR, Zavery A, Aaronson PI. Endothelium -dependent relaxation of rat aorta and main pulmonary artery by the phytoestrogens genistein and daidzein. Cardiovasc. Res. 2000;46:539-546. https://doi.org/10.1016/S0008-6363(00)00049-3
  26. Latonnelle K, Menn FL, Kaushik SJ, Bennetau-Pelissero C. Effects of dietary phytoestrogens in vivo and in vitro in rainbow trout and siberian sturgeon: interest and limits of the in vitro studies of interspecies differences. Gen. Comp. Endocrinol. 2002;126:39-41. https://doi.org/10.1006/gcen.2001.7773
  27. Nevala R, Korpela R, Vapaatalo H. Plant-derived estrogens relax rat mescenteric artery in vitro. Life Sci. 1998;63:95-100.
  28. Tomioka H, Hattori Y, Fukao M, Sato A, Liu M, Sakuma I, Kitabatake A, Kanno M. Relaxation in different-sized rat blood vessels mediated by endothelium-derived hyperpolarizing factor: importance of processes mediating precontractions. J. Vasc. Res. 1999;36:311-320. https://doi.org/10.1159/000025659
  29. Nagao T, Illiano S, Vanhoutte PM. Heterogeneous distribution of endothelium-dependent relaxations resistant to NG-nitro-L-arginine in rats. Am. J. Physiol. 1992;263:1090-1094.