한국축산시설환경학회지 (Journal of Animal Environmental Science)

한국축산환경학회 (The Korean Society of Animal Environmental Science & Technology)
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백하수오 추출물이 In vitro 반추위 발효성상 및 메탄가스 생성에 미치는 영향

Effects of Cynanchum Wilfordii Extract on In vitro Ruminal Fermentation Characteristics and Methane Production

  • 양승학 (농촌진흥청 국립축산과학원) ;
  • 임정수 (농촌진흥청 국립축산과학원) ;
  • 김별 (농촌진흥청 국립축산과학원) ;
  • 황옥화 (농촌진흥청 국립축산과학원) ;
  • 조성백 (농촌진흥청 국립축산과학원) ;
  • 최동윤 (농촌진흥청 국립축산과학원) ;
  • 최석근 (국립한경대학교) ;
  • 황성구 (국립한경대학교)
  • 투고 : 2013.11.30
  • 심사 : 2013.12.19
  • 발행 : 2013.12.30
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초록

The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.

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참고문헌

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