한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제28권4호
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  • Pages.349-353
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  • 2013
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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Effects of Storage in Different Commercial Semen Extenders on Sperm Motility, Viability and Membrane Integrity of Korean Native Boar Spermatozoa

  • Sa, Soo-Jin (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, In-Cheul (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Hong, Joon-Ki (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Du-Wan (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Cho, Kyu-Ho (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Young-Hwa (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Chung, Ki-Hwa (Gyeongnam National University of Science and Technology) ;
  • Park, Jun-Cheol (Swine Science Division, National Institute of Animal Science, RDA)
  • 투고 : 2013.11.02
  • 심사 : 2013.11.18
  • 발행 : 2013.12.31
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초록

The objective of this study was to compare the effect of semen extenders on the sperm motility, viability, acrosome integrity and functional integrity of plasma membrane (HOST: hypo-osmotic swelling test) during liquid preservation of Korean Native boar semen. In this experiment, semen was diluted in Androhep plus, Beltsville Thawing Solution (BTS), ModenaTM, Seminark and Vitasem LD. Sperm-rich fractions were collected from three Korean Native boars and sub-samples were diluted ($30{\times}10^6$ spermatozoa/ml) in different semen extenders. Semen samples were stored at $17^{\circ}C$ for 96 hours. On everyday (0, 24, 48, 72, 96 h) after storage, the sperm characteristics relevant for fertility, such as sperm motility, viability, acrosome integrity and HOST positive were evaluated. The motility of spermatozoa stored in different extenders was no significantly different among other extenders (P>0.05). Also, no difference was observed among samples processed with different extenders in the percentage of sperm viability, acrosome integrity and HOST positive. All extenders maintained a high percentage (70%) of sperm motility, viability and acrosome integrity through 96 h of storage. The result of this study show that there was no significant differences among extenders in their capacity to preserve motility, viability and membrane integrity of spermatozoa from normal, fertile Korean Native boars for 96 h of liquid preservation at $17^{\circ}C$.

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참고문헌

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