Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Rapid Detection Kit for Staphylococcus aureus

황색포도상구균 신속 검출용 간이 진단키트

  • Kim, Gi-Young (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Park, Saet-Byeol (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Moon, Ji-Hea (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Lee, Sang-Dae (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Lee, Sea-Rom (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Jang, Youn-Jung (Department of Agricultural Engineering, National Academy of Agricultural Sciences)
  • 김기영 (국립농업과학원 농업공학부) ;
  • 박샛별 (국립농업과학원 농업공학부) ;
  • 문지혜 (국립농업과학원 농업공학부) ;
  • 이상대 (국립농업과학원 농업공학부) ;
  • 이새롬 (국립농업과학원 농업공학부) ;
  • 장윤정 (국립농업과학원 농업공학부)
  • Received : 2013.04.01
  • Accepted : 2013.05.15
  • Published : 2013.06.30
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Abstract

This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect $10^5$ cfu/mL of Staphylococcus in chicken meat extract.

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References

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