농업과학연구 (Korean Journal of Agricultural Science)

  • 제40권2호
  • /
  • Pages.115-121
  • /
  • 2013
  • /
  • 2466-2402(pISSN)
  • /
  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
권호 표지
DOI QR코드

DOI QR Code

세엽 한국들잔디 변이체 식별을 위한 SCAR 마커 개발

SCAR markers were developed to identify zoysiagrass mutants exhibiting fine leaf characteristics

  • Chung, Sung Jin (Department of Horticulture, Chungnam National University) ;
  • Park, Su Jeong (Department of Horticulture, Chungnam National University) ;
  • Choi, Young In (Department of Horticulture, Chungnam National University) ;
  • Kim, In-Kyung (Department of Horticulture, Chungnam National University) ;
  • Lee, Ka-Yeon (Department of Horticulture, Chungnam National University) ;
  • Kim, Hun-Joong (Department of Horticulture, Chungnam National University) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University)
  • 투고 : 2013.06.04
  • 심사 : 2013.06.17
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Polymorphic bands of two fine-leaf zoysiagrass mutants (CNU 70-1, CNU 70-2) induced via a gamma-ray irradiation on seeds of Zoysia japonica were obtained by using randomly amplified polymorphic DNA (RAPD) primers. The genotype-specific fragments were then converted into PCR-based sequence characterized amplified region (SCAR) markers, which are now amenable to detecting them among other zoysiagrass species widely noticeable in Korea. The CNU 70-1-specific primer set amplified about 900 bp successfully, while the CNU 70-6 marker produced the expected 1,500 bp band, by which those markers were nominated by CNU 70-1_900 and CNU 70-6_1500 SCARs, respectively. The developed SCAR markers can be an applicable tool in sod industry where illegal appropriation hampers breeder's right and profits due to the turfgrass plant vegetatively propagating.

키워드

참고문헌

  1. Abraham E, Aa M, Honig J, Kubik C, Bonos SA. 2005. The use of scar markers to identify Texas x Kentucky bluegrass hybrids. International Turfgrass Society Research Journal 10:495-500.
  2. Bae EJ, Park NC, Lee KS, Lee SM, Choi JS, Yang GM. 2010. Distribution and morphology characteristics of native zoysiagrasses (Zoysia spp.) grown in south Korea. Korea Turfgrass Science 245:97-105.
  3. Beard JB. 1973. Turfgrass:science and culture. Prentice-Hall, Englewood Cliffs, NJ, USA.
  4. Curley J, Jung G. 2004. RAPD-based genetic relationships in Kentucky bluegrass: comparison of cultivars, interspecific hybrids, and plant introduction. Crop Science 44:1299-1306. https://doi.org/10.2135/cropsci2004.1299
  5. Fard JRF, Moghaddam MRF, Kafi M. 2012. Evaluation of genetic diversity among some genotypes of Kentucky bluegrass by RAPD molecular markers. Horticulture, Environment, and Biotechnology 53:298-303. https://doi.org/10.1007/s13580-012-0120-5
  6. Haydu JJ, Hodges AW, Hall CR. 2006. Economic impacts of the turfgrass and lawncare industry in the United States. TurfNews 30:52, 54-56.
  7. Hyun SJ, Shim SR, Ahn BJ. 2010. Analysis of hybridization in Korean ecotypes of Poa pratensis L. using RAPD and SCAR markers. Korean Journal of Horticultural Science & Technology 28:281-287.
  8. Kang BC, Kamkung Y, Shin HK. 1999. Analysis of the genetic variation in Anyang Joonggi and development of Anyang Joonggi specific DNA marker. Korean Journal of Horticultural Science & Technology 17:197.
  9. Kang SY, Lee GJ, Lim KB, Lee HJ, Park IS, Chung SJ, Kim JB, Rhee HK. 2008. Genetic diversity among Korean bermudagrass (Cynodon spp.) ecotypes characterized by morphological, cytological and molecular approaches. Molecules and Cells 25:163-171.
  10. Kumar NS, Gurusubramanian G. 2011. Random amplified polymorphic DNA (RAPD) markers and its applications. Science Vision 11:116-124. https://doi.org/10.1167/11.11.116
  11. Lee, GJ. 2008a. Integration of breeding efforts for high-valued turfgrass in Korea. Summer International Turfgrass Symposium in 2011-turfgrass research in changeable weather. Asia Turfgrass Society Symposium 45-67.
  12. Lee HJ, Lee GJ, Kim DS, Kim JB, Ku JH, Kang SY. 2008b. Determination of the optimum dose range for a mutation induction of turfgrasses by a gamma-ray. Korea Turfgrass Science 22:25-34.
  13. Lee HJ, Lee GJ, Kim DS, Kim JB, Ku JH, Kang SY. 2008c. Selection and physiological characterization of glyphosatetolerant zoysiagrass mutants derived from a gamma ray irradiation. Korean Journal of Horticultural Science & Technology 26:454-463.
  14. Ministry of Agriculture and Forestry. 2003. Agricultural and forestry statistical yearbook.
  15. Rajasekar S, Fei SZ, Christians NE. 2007. Analysis of genetic diversity in colonial bentgrass (Agrostis capillaries L.) using randomly amplified polymorphic DNA (RAPD) markers. Genetic Resources and Crop Evolution 54:45-53. https://doi.org/10.1007/s10722-005-1883-7
  16. Scheef EA, Casler MD, Jung G. 2003. Development of species-specific SCAR markers in bentgrass. Crop Science 43:345-349. https://doi.org/10.2135/cropsci2003.0345

피인용 문헌

  1. Development of ‘Halla Green 7’: A Zoysiagrass Cultivar with Greening Period Extending Phenotype at Lower Temperatures vol.53, pp.4, 2013, https://doi.org/10.9787/kjbs.2021.53.4.458
  2. Development of Cultivar ‘Halla Green 10’ in Zoysiagrass vol.53, pp.4, 2021, https://doi.org/10.9787/kjbs.2021.53.4.467