The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Pencycuron in Agricultural Products by Simultaneous Determination

동시 다성분 분석법에 의한 농산물 중 펜시쿠론 분석

  • Park, Young-Hye (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Chang-Ho (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Ae-Kyung (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Shin, Jae-Min (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Jae-Kyoo (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Park, Sun-Hee (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Yun, Eun-Sun (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Mu-Sang (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chae, Young-Zoo (Gangnam Agro-Fishery Products Inspection Center, Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 박영혜 (서울시보건환경연구원 강남검사소) ;
  • 한창호 (서울시보건환경연구원 강남검사소) ;
  • 김애경 (서울시보건환경연구원 강남검사소) ;
  • 신재민 (서울시보건환경연구원 강남검사소) ;
  • 이재규 (서울시보건환경연구원 강남검사소) ;
  • 박선희 (서울시보건환경연구원 강남검사소) ;
  • 윤은선 (서울시보건환경연구원 강남검사소) ;
  • 김무상 (서울시보건환경연구원 강남검사소) ;
  • 채영주 (서울시보건환경연구원 강남검사소)
  • Received : 2013.07.05
  • Accepted : 2013.08.09
  • Published : 2013.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

An analytical method for the simultaneous determination of pencycuron in agricultural products was established by using GC-NPD. The method was validated through the guidelines of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. The calibration curve of pencycuron was linear over the concentration range of 0.02-2 mg/kg with correlation coefficient of above 0.9999. The limits of detection and quantification were 0.005 and 0.02 mg/kg. Mean recoveries of pencycuron for each sample were 79.6-107.5% at the level of 0.02 mg/kg and 86.4-112.1% at the level of 0.2 mg/kg and 84.0- 104.9% at the level of 1 mg/kg. Relative standard deviation (RSD) in recoveries were all less than 5%. The intra- and inter-day precision (RSD) were 1.6-3.0% and 5.3-11.5%, respectively. The result of validation indicated that this method was accurate and sensitive assay.

GC-NPD를 이용하여 pencycuron의 다종농약다성분 분석법을 확립하고자 본 연구를 수행하였다. 제시된 분석법의 검증을 위해 예비 실험을 거쳐 농약이 검출되지 않은 상추, 시금치, 엇갈이배추, 근대, 아욱, 배추를 대상으로 실험하였다. Pencycuron 표준용액을 0.02-2 mg/kg의 농도로 희석하여 분석한 검량선의 직선성($r^2$ = 0.9999)은 우수하였다. 검출한계는 0.005 mg/kg이고 정량한계는 0.02 mg/kg이며 회수율은 79.6-112.1%, 분석오차는 최대 4.9%미만으로 잔류농약 분석법에서 허용되는 회수율 기준 70-120% 범위와 분석오차 20% 이하의 기준에 적합하였다. 정밀도는 intra-day test에서 1.6-3.0%, inter-day test에서 5.3-11.5%를 나타내어 다종농약다성분 분석법을 통해 pencycuron의 직선성, 검출한계, 정량한계, 회수율과 정밀도를 검증하고 정확성을 증명하였다. 오랜 시간이 걸려 추출, 정제, 유도체화 과정을 거치는 분석법보다 빠르고 간편한 실험법인 다종농약다성분 분석법이 pencycuron의 분석시에 유용한 분석법으로 활용될 수 있을 것으로 판단된다.

Keywords

References

  1. Central Agricultural Pesticides Laboratory http://www.capl.sci.eg/ActiveIngredient/Pencycuron.html#physical
  2. Cho, I. S., L. H. Hwang, T. H. Cho, B. C. Choe, Y. H. Park, H. Y. Park, H. J. Kim and J. H. Kim (2010) Current status of pesticide residues in commercial agricultural products in Gangseo, Seoul (2010). Report of S. I. H. E. 46:20-32.
  3. Choe, K. C., N. C. Sung, Y. H. Ahn, J. G. Kim and S. Y. Yoo (2009) Introductory Environmental Science. pp.191-192. Donghwa Technology Publishing Co, Koera.
  4. Jang, M. R., H. K. Moon, T. R. Kim, D. H. Yuk, E. H. Kim, C. K. Hong, C. M. Choi, I. S. Hwang, J. H. Kim and M. S. Kim. (2011) The survey on pesticide residues in vegetables collected in Seoul. J. Korean Pesticide Science, 15:114-124.
  5. Jeon, J. S., M. J. Kwon, S. H. O, H. J. Nam, H. Y. Kim, J. M. Go and Y. H. Kim (2006) A survey on the pesticide residues on agricultural products on the markets in Incheon area from 2003 to 2005. J. Environmental Agriculture, 25:180-189. https://doi.org/10.5338/KJEA.2006.25.2.180
  6. Jung, Y. H., J. E. Kim, J. H. Kim, Y. D. Lee, C. H. Lim and J. H. Her (2004) Pesticide Science (The newest). pp. 213. Sigmapress, Korea.
  7. Kataria, H. R. and P. R. Verma (1990) Efficacy of fungicidal seed treatments against pre-emergence damping-off and post-emergence seedling root rot of growth chamber grown canola caused by Rhizoctonia solani AG-2-1 and AG-4. Can. J. Plant Pathol. 12:409-416. https://doi.org/10.1080/07060669009500982
  8. Korean Crop Protection Association (2011) Guideline of pesticides Use. pp. 322-323. Samjeong Press Inc, Korea.
  9. Korea Food and Drug Administration (2011) Korea Food Code, pp. 10-4-10-10-4-12, Korea Food and Drug Administration, Korea.
  10. Korea Food and Drug Administration (2011) Korea Food Code, pp. 10-4-83-10-4-85, Korea Food and Drug Administration, Korea.
  11. Kuck, K. H., I. Ueyama, S. Kurogochi and Y. Yamada (1988) Biological and biochemical aspects of Monceren(R) (Pencycuron), a Rhizoctonia specific fungicide. Proceedings of the fifth International Congress on Plant Pathology, p. 22.
  12. Lee, Y. D. (2012) Handbook for the pestcide resdue analytical methods of food code, 3rd ed, National Institution of Food and Drug Safety Evaluation, Cheongwon, Korea.
  13. Lee, S. M., Michael L, Papathakis, Hsiaoming C, Feng, Gray FH, Joyce EC (1991) Multipesticide residue method for fruits and vegetables. Fresenius J. Anal. Chem 339-376.
  14. Pal, R., K. Chakrabarti, A. Chakraborty and A. Chowdhury (2005) Dissipation of pencycuron in rice plant. Journal of Zhejiang University, 6(8):1220-1223, Zhejiang University Press. https://doi.org/10.1631/jzus.2005.B1220
  15. Roberts, D. L., and C. T. Stephens (1984) Sensitivity of Rhizoctonia solani to experimental fungicide NTN 19701. Phytopathology 7:854.
  16. Sumner, D. R., J. E. Hook, N. A. Minton, J. L. Crawford and C. C. Dowler (1984) Control of crown and brace root rot of corn with soil fungicides. Phytopathology 74:633.
  17. Sumner, D. R. (1987) Efficacy of pencycuron against isolates representing different anastomosis groups of Rhizoctonia solani and Rhizoctonia-like binucleate fungi. Plant Dis. 71:515-518. https://doi.org/10.1094/PD-71-0515
  18. Tomlin, C. D. S. (Ed.) (1997) Pencycuron, 11th ed.. The Pesticide Manual British Crop Protection Council, UK, pp. 925-937.
  19. Yun, W. K. (2006) Simultaneous determination of pencycuron, flutolanil, in ginseng using Triple-quadropole LC-MS/MS and GC. Seoul National University of Technology Graduated School of Industry and Engineering.