한국전자통신학회논문지 (The Journal of the Korea institute of electronic communication sciences)

한국전자통신학회 (Korea Institute of Electronic Communication Science)
권호 표지
DOI QR코드

DOI QR Code

전뇌허혈 유발 후 침전극저주파자극 적용이 소뇌의 세포자멸사 억제에 미치는 영향

The Effect of Needle Electrode Electrical Stimulation Following Global Ischemia on the Suppression of Apoptosis in the Cerebellum

  • 왕중산 (여주대학교 물리치료과) ;
  • 박주현 (수원여자대학교 물리치료과) ;
  • 문옥곤 (군장대학교 물리치료과) ;
  • 김년준 (포항대학교 물리치료과) ;
  • 최정현 (남서울대학교 물리치료학과) ;
  • 김지성 (수원여자대학교 물리치료과)
  • 투고 : 2013.10.28
  • 심사 : 2013.12.16
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 온목동맥의 일시적 폐색에 의해 유발된 전뇌허혈로 인해 발생한 SD 랫드 소뇌 부위의 세포자멸사에 대한 침전극저주파자극(Needle Electrode Electrical Stimulation : NEES) 적용의 효과를 알아보기 위하여 면역조직화학적검사법을 통하여 조직을 관찰하였다. 연구를 통하여 대조군과 전뇌허혈유발군, 침전극저주파자극시행군의 세포자멸사 관련 인자의 발현정도를 비교하여 분석한 결과 전뇌허혈 후 침전극저주파의 적용은 SD 랫드 소뇌 부위의 c-fos 발현 감소에 유의한 효과를 보였으며, bax, caspase-3의 발현 감소에는 유의한 영향이 없는 것으로 나타났다. 이상의 결과로 보아 전뇌허혈 후 침전극저주파자극의 적용은 세포자멸사 관련 인자의 발현 억제에 일부분 효과성이 있는 것으로 사료된다.

This study conducted observation of tissues using the immunohistochemistry methods in order to see the effect of Needle Electrode Electrical Stimulation(: NEES) on apoptosis in the SD rat cerebellum caused by global ischemia which came from common carotid artery occlusion. The comparison and analysis results of expression of factors related to apoptosis among the control group, the global ischemia group and the NEES group showed that NEES following global ischemia had a significant effect on the reduction of c-fos expression in the SD rat cerebellum, and didn't have a significant effect on the reduction of bax and caspase-3 expression. In conclusion, it is considered that NEES following global ischemia is partially effective in suppressing the expression of factors related to apoptosis.

키워드

참고문헌

  1. Chiarugi A, "Poly(ADP-ribosyl)ation and stroke", Journal of Pharmacology Research, Vol. 52, pp. 15-24, 2005. https://doi.org/10.1016/j.phrs.2005.02.018
  2. Sharma S.S, and Thiyagarajan M, "Molecular mechanism of cerebral ischemia", In: Thakur M.K, Prasad S., editors. Molecular and Cellular Neurobiology. New Delhi: Narosa Publishing House, pp. 191-202, 2005.
  3. Eun-Jin Seo, Hyun-Hui Beak, Jeong-Ja Kim, "The Effect of Direct Swallowing Treatment on the Swallowing Function and Quality of Life of the Stroke Patient", The Journal of the Korea Institute of Electronic Communication Science, Vol. 8, No. 9, pp. 1413-1420, 2013. https://doi.org/10.13067/JKIECS.2013.8.9.1413
  4. Yun-Hee Son, Ji-On Park, Hyun-Sook Hwang, "Research on the Effects of the Dementia Prevention Program on the Retired Seniors in the Industrial Age", The Journal of the Korea Institute of Electronic Communication Science, Vol. 8, No. 10, 1601-1608, 2013. https://doi.org/10.13067/JKIECS.2013.8.10.1601
  5. Sun-Mi Park, "The Relationship between Dysmenorrhea and Alternative Medicine among the Internet Age", The Journal of The Korea Institute of Electronic Communication Sciences, Vol. 8, No. 7, pp. 1108-1109, 2012.
  6. Petito C.K, Feldmann E., Pulsinelli W.A. and Plum F, "Delayed hippocampal damage in humans following cardiorespiratory arrest", Journal of Neurology, Vol. 37, pp. 1281-1286, 1987. https://doi.org/10.1212/WNL.37.8.1281
  7. Lim C, Alexander M.P, LaFleche G, Schnyer D.M. and Verfaellie M, "The neurological and cognitive sequelae of cardiac arrest", Journal of Neurology, Vol. 63, pp. 1774-1778, 2004. https://doi.org/10.1212/01.WNL.0000144189.83077.8E
  8. Osuullivan Susan B, and Schmitz Thomas J, "Physical rehabilitation : Assessment and Treatment. 3ed", Philadelphia, F.A Davis Company, 1994.
  9. Jang M.H, Shin M.C, Koo G.S, Lee C.Y, Kim E.H, and Kim C.J, "Acupuncture decrease nitric oxide synthase expression in periaqueductal gray area of rats with streptozotocin-induced diabetes", Journal of Neuroscience, Vol. 337, pp. 155-158, 2003.
  10. Vaux D.L, and Strasser A, "Molecular biology of apoptosis", Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, pp. 2239-2244, 1996. https://doi.org/10.1073/pnas.93.6.2239
  11. Jacobson M.D, Weil M. and Raff M.C, "Programmed cell death in animal development", Journal of Cell, Vol. 88, pp. 347-354, 1997. https://doi.org/10.1016/S0092-8674(00)81873-5
  12. Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L, Isakson P., "Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain", Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 25, pp. 12013-12017, 1994. https://doi.org/10.1073/pnas.91.25.12013
  13. Leak R. and Broderick J.E, "Treatment efficacy of acupuncture : a review of the research literature", Journal of Intergrative medicine, Vol. 1, pp. 107-115, 1998.
  14. Uchida S, Kagitani F, Suzuki A. and Aikawa Y, "Effect of acupuncture like stimulation on cortical cerebral blood flow in anesthetized rats", Japan Journal of Physiology, Vol. 50, pp. 495-507, 2000. https://doi.org/10.2170/jjphysiol.50.495
  15. Gao H, Guo J, Ahao P. and Cheng J, "The neuroprotective effect of electro- acupuncture on focal cerebral ischemia in monkey", Journal of Acupuncture Electro-Therapy Research, Vol. 27, pp. 45-57, 2002. https://doi.org/10.3727/036012902816026112
  16. Kim E.H, Kim Y.J, Lee H.J, Huh Y, Chung J.H, Seo J.C, Kang J.E, Lee H.J, Yim S.V. and Kim C.J, "Acupuncture increase cell proliferation in dentate gyrus after transient global ischemia in gerbils", Journal of Neuroscience Letter, Vol. 277, pp. 21-24, 2001.
  17. Ying S.X. and Cheng J.S, "Effects of electroacupuncture on C-fos expression in gerbils hippocampus during transient global ischemia", Journal of Acupuncture Electro- Therapy Research, Vol. 19, No. 4, pp. 207-213, 1994. https://doi.org/10.3727/036012994816357240
  18. Chen Y.M. and Fang Y.A, "108 cases of hemiplegia caused by stroke: the relationship between CT scan results, clinical findings and the effect of acupuncture treatment", Journal of Acupuncture Electro-Therapy Research, Vol. 15, No. 1, pp. 9-17, 1990. https://doi.org/10.3727/036012990816358298
  19. Luo H., Meng F, Jia Y. and Zhao X, "Clinical research on the therapeutic effect of the electro-acupuncture treatment in patients with depression", Journal of Psychiatry Clinical Neuroscience, Vol. 52, pp. S338-40, 1998. https://doi.org/10.1111/j.1440-1819.1998.tb03262.x
  20. Ulett G.A, Han S. and Han J.S, "Electroacu -puncture: mechanisms and clinical application", Journal of Biology & Psychiatry, Vol. 44, No. 2, pp. 129-38, 1998. https://doi.org/10.1016/S0006-3223(97)00394-6
  21. Wong A.M, Su T.Y, Tang F.T, Cheng P.T. and Liaw M.Y, "Clinical trial of electrical acupuncture on hemiplegic stroke patients", American journal of physical medicine & rehabilitation, Vol. 78, No. 2, pp. 117-22, 1999. https://doi.org/10.1097/00002060-199903000-00006
  22. Kyung-.Ok Min, "Electrotherapy", Hyunmoonsa, pp.52-82, 466-492, 2001.
  23. Wei G.W, Huang Y.L, Wu G.C. and Cao X.D, "Regulation of glial cell line-derived neurotrophic factor expression by electroacupuncture after transient focal ischemia", Journal of Acupuncture Electro-Therapy Research, Vol. 25, pp. 81-90, 2000. https://doi.org/10.3727/036012900816356208
  24. Geon-Mok Lee, Yoo-Sun Yang, Sung-Jin Kim, Yoo-Jin Hwang, Dong-Soo Lyu, Min-Ja Kim, Eun-Hee Cho, Hyun-Jung Kim, Myeong-Bok Yang, Beung-Cheul Lee, In Lee, "The nuclear medical study on the effect of Hap-Kok(LI4) Acupuncture on cerebral blood flow ", Journal of Korean Acupuncture & Moxibustion Medicine Society, Vol. 18, No. 6, 2001.
  25. Pang J, Itano Y, Sumitani K, Negi T. and Miyamoto O, "Electroacupuncture attenuates both glutamate release and hyperemia after transient ischemia in gerbils", American Journal of Chinese Medicine, Vol. 31, No. 2, pp. 295-303, 2003. https://doi.org/10.1142/S0192415X03000977
  26. Sugawara T, Lewen A, Noshita N, Gaschie Y. and Chan P.H, "Effect of global ischemia duration on neuronal, astroglial, oligodendroglial, and microglial reactions in the vulnerable hippocampal CA1 subregion in rats", Journal of Neurotrauma, Vol. 19, pp. 85-98, 2002. https://doi.org/10.1089/089771502753460268
  27. Compton M.M. and Cidlowski J.A, "Rapid in vivo effect of glucocorticoids on the integrity of rat lymphocyte genomic DNA", Journal of Endocrinology, Vol. 118, pp. 38-45, 1994.
  28. Wang S, Miura M, Y-K Hong Z, Galiardini V, Shi I., Greenberg A. and Yuan J, "Identification and characterization of Ich-3, a member of the ICE/Ced-3 family and an upstream regulator of ICE", Journal of Biology & Chemistry, Vol. 271, pp. 20580-20587, 1996. https://doi.org/10.1074/jbc.271.34.20580
  29. Orth K, O'Rourke K, Salvesen G.S. and Dixit V.M, "Molecular ordering of apoptotic mammalian CED/ICE like protease", Journal of Biology & Chemistry, Vol. 271, pp. 20977-20980, 1996. https://doi.org/10.1074/jbc.271.35.20977
  30. Saleh A, Srinivasula S.M, Acharya S, Fishel R. and Alnemri E.S, "Cytochrome c and dATP-mediated oligomrization of apaf-1 is a prerequisite for procaspase-9 activation", Journal of Biology & Chemistry, Vol. 274, pp. 17941-17945, 1999. https://doi.org/10.1074/jbc.274.25.17941
  31. Rao RV, Peel A, Logvinova A, Rio G.D, Hermelm E., Yokota T, Goldsmith P.C, Ellerby L.M, Ellerby H.M. and Bredesen D.E, "Coupling endoplasmic reticulum stress to the cell death program : role of the ER chaperone GRP78", Federation of American Societies for Experimental Biology, Vol. 514, pp. 122-128, 2002. https://doi.org/10.1016/S0014-5793(02)02289-5
  32. Graham S.H. and Chen J, "Programmed cell death in cerebral ischemia", Journal of Cerebral Blood Flow Metabolism, Vol. 21, pp. 99-109, 2001. https://doi.org/10.1097/00004647-200102000-00001
  33. Susin S.A, Daugas E, Ravagnam L, Samejima K, Zamzami N, Loeffler M, Costantini P, Ferri K.F, Irinopoulou T, Prevost M.C, Brothers G, Mak T.W, Peninger J, Earnshaw W.C. and Kroemer G, "Two distict pathways leading to nuclear apoptosis", Journal of Experimental Medicine, Vol. 192, pp. 571-580, 2000. https://doi.org/10.1084/jem.192.4.571
  34. Daugas E, Susin S.A, Zamzami N, Ferri K.F, Irinopoulou T, Larochette N, Prevost M.C, Leber B, Andrew D, Penninger J. and Kroemer G, "Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. Federation of American Societies for Experimental Biology, Vol. 14, pp. 729-739, 2000. https://doi.org/10.1096/fasebj.14.5.729
  35. Chung J.H, Lee E.Y, Jang M.H, Kim J, Kim C.J, Ha E, Park H.K, Choi S, Lee H, Park S.H, Leem K.H, and Kim E.H, "Acupuncture decreases ischemia-induced apoptosis and cell proliferation in dentate gyrus of gerbils", Journal of Neurological Research, Vol. 29, No. 1, pp. 23-27, 2007. https://doi.org/10.1179/016164107X172239
  36. Jang M.H, Shin M.C, Lee T.H, Lim B.V, Shin M.S, Min B.I, Kim H, Cho S, Kim E.H. and Kim C.J, "Acupuncture suppresses ischemiainduced increase in c-Fos expression and apoptosis in the hippocampal CA1 region in gerbils. Journal of Neuroscience, Vol. 347, No. 1, pp. 5-8, 2003(b).
  37. Yang Y.J, Kim Y.S, Shin M.S, Chang H.K, Lee T.H, Sim Y.J, Shin M.C, Park H.Y, Kim S, Park H.K. and Kim C.J, "Effects of acupuncture on the intrastriatal hemorrhageinduced caspase-3 expression and newly cell birth in rats" Journal of Neurological Research, Vol. 29, No. 1, pp. S65-71, 2007. https://doi.org/10.1179/016164107X172266
  38. Wang SJ, Omori N, Li F, Jin G, Zhang WR, Hamakawa Y, Sato K, Nagano I, Shoji M. and Abe K, "Potentiation of Akt and suppression of caspase-9 activations by electroacupuncture after transient middle cerebral artery occlusion in rats", Journal of Neuroscience, Vol. 331, No. 2, pp. 115-118, 2002.
  39. Soo-Han Kim "The effect of needle electrode electrical stimulation on the change of caspase-3, 9 and neuronal nitric oxide synthase immunoreactive cells in the sprague dawley rats ", Hanseo University A doctor's degree, 2004.

피인용 문헌

  1. Current Research Trend on Acupuncture Treatment for Nerve Regeneration and Recovery: Based on the Data of PubMed vol.31, pp.4, 2014, https://doi.org/10.14406/acu.2014.026