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Changes in the Levels of γ-Aminobutyric Acid and Free Amino Acids during Kimchi Fermentation

김치 발효 숙성 중의 γ-aminobutyric acid (GABA) 및 아미노산 함량의 변화

  • Lee, Hye-Hyun (Dept. of Food and Nutrition, Duksung Women's University) ;
  • Kim, Gun-Hee (Dept. of Food and Nutrition, Duksung Women's University)
  • 이혜현 (덕성여자대학교 식품영양학과) ;
  • 김건희 (덕성여자대학교 식품영양학과)
  • Received : 2013.06.19
  • Accepted : 2013.11.04
  • Published : 2013.12.31

Abstract

The objective of this study was to examine the levels of free amino acids and identify the correlation between ${\gamma}$-aminobutyric acid (GABA)and L-glutamic acid contents in Kimchi during fermentation. During 2 weeks of fermentation, the acidity of Kinchi increased, i.e., the pH level decreased from 5.24 to 4.40. The content of amino acids determined using HPLC differed significantly (p < 0.05) during 7 weeks of fermentation. Over the 7 weeks of fermentation, the content of most free amino acids increased in the order L-valine > L-glutamic acid > L-glycine, except L-methionine decreased. Initially, the GABA content was found to be $72.43{\mu}M/100g$ fresh weight (fw), and it increased to $229.06{\mu}M/100g$ fw after 7 weeks. This rapid increase in the GABA content in the initial stage is considered to be due to L-glutamic acid. However, during the period of 0~7 weeks, no correlations were found between the L-glutamic acid and GABA contents.

Keywords

References

  1. Aoki H, Furuya Y, Endo Y, Fujimoto K. 2003. Effect of gamma-aminobutyric acid-enriched tempeh-like fermented soybean (GABA-Tempeh) on the blood pressure of spontaneously hypertensive rats. Biosci Biotech Biochem 67(8): 1806-1808 https://doi.org/10.1271/bbb.67.1806
  2. Bae MO, Kim HJ, Cha YS, Lee MK, Oh SH. 2009. Effects of Kimchi lactic acid bacteria Lactobacillus Sp. OPK2-59 with high GABA producing capacity on liver function improvement. J Korean Soc Food Sci Nutr 38(11): 1499-1505 https://doi.org/10.3746/jkfn.2009.38.11.1499
  3. Breitkreuz KE, Shelp BJ. 1995. Subcellular compartmentation of the 4-aminobutyrate shunt in protoplasts from developing soybean cotyledons. Plant Physiol 108(1): 99-103 https://doi.org/10.1104/pp.108.1.99
  4. Cha YS, Oh SH. 2000. Investigation of gamma-aminobutyric acid in Chinese cabbages and effects of the cabbage diets on lipid metabolism and liver function of rats administered with ethanol. J Korean Soc Food Sci Nutr 29(3): 500-505
  5. Chang MS, Cho SD, Bae DH, Kim GH. 2010. Safety and quality assessment of Kimchi made using various salts. Korean J Food Sci Technol 42(2): 160-164
  6. Cho Y, Lee HS. 1979. A study on flavorous taste components in Kimchis on free amino acids. Korean J Food Sci Technol 11(1): 26-31
  7. Dhakal R, Bajpai V, Baek K. 2012. Production of GABA(${\gamma}$-aminobutric acid) by microorganisms: a review. Brazilian J Microbiol 43(4): 1230-1241 https://doi.org/10.1590/S1517-83822012000400001
  8. Hong EY, Kim SJ, Kim GH. 2011. Identification and quantitative determination of glucosinolates in seeds and edible parts of Korean Chinese cabbage. Food Chem 128(4): 1115-1120 https://doi.org/10.1016/j.foodchem.2010.11.102
  9. Hong EY, Kim GH. 2005. Changes in vitamin U and amino acid levels of Korean Chinese cabbages during Kimchi fermentation. Korean J Food Preserv 12(4): 411-416
  10. Hwang ES, Jang MR, Kim GH. 2012. Effects of storage condition on the bioactive compound contents of Korean cabbage. Food Sci Biotech 21(6): 1655-1661 https://doi.org/10.1007/s10068-012-0220-0
  11. Jang MS, Park HY, Park JI, Byun HS, Kim YK, Yoon HD. 2011. Analysis of nutrient composition of Baechu Kimchi (Chinese cabbage kimchi) with seafoods. Korean J Food Preserv 18(4): 535-545 https://doi.org/10.11002/kjfp.2011.18.4.535
  12. Kang SS, Kim JM, Byun MW. 1988. Preservation of Kimchi by ionizing radiation. Kor J Food Hygiene 3(4): 225-232
  13. Kang TJ, Oh SH. 2007. GABA production and use. BioWave, 9(14):3. Available from: http://bric.postech.ac.kr/myboard/read.php?Board=review0&id=1871. Accessed Mar 17, 2009
  14. Kim GH. 2003. Determination of vitamin U in food plants. Food Sci Technol Res 9(4): 316-319 https://doi.org/10.3136/fstr.9.316
  15. Kim JW. 2008. Quality changes of Gamdongjeotmu Kimchi during storage with different temperatures and periods of fermentation. Master's thesis. Ewha Womans University. pp. 27-28
  16. Kim MJ, Kim KS. 2012. Isolation and identification of ${\gamma}$-aminobutyric acid (GABA)-producing lactic acid bacteria from Kimchi. J Korean Soc Appl Biol Chem 55(6): 777-785 https://doi.org/10.1007/s13765-012-2174-6
  17. Kook MC. 2012. Production of GABA-rich tomato paste by Lactobacillus sp. fermentation. Korean J Food Nutr 25(1): 26-31 https://doi.org/10.9799/ksfan.2012.25.1.026
  18. KCDC (Korea Centers for Disease Control & Prevention). Korea Health statistics 2011 :Korea National Health and Nutrition Examination Survey(KNHANESV-2). Ministry of Health and Welfare. Seoul. p. 274, p. 302
  19. Lee HO, Lee HJ, Woo SJ. 1994. Effect of cooked glutinous rice flour and soused shrimp on the changes of free amino acid, total vitamin Cand ascorbic acid contents during Kimchi fermentation. Korean J Food Cookery Sci 10(3): 225-231
  20. Lee YK, Lee MY, Kim SD. 2004. Effect of monosodium glutamate and temperature change on the content of free amino acids in Kimchi. J Korean Soc Food Sci Nutr 33(2): 399-404 https://doi.org/10.3746/jkfn.2004.33.2.399
  21. Li H, Cao Y. 2010. Lactic acid bacterial cell factories for gamma-aminobutyric acid. Amino Acids 39(5): 1107-1116 https://doi.org/10.1007/s00726-010-0582-7
  22. Lim JH, Park SS, Jeong SW, Park KJ, Seo KH, Sung JM. 2013. Quality characteristics of Kimchi fermented with abalone or sea tangle extracts. J Korean Soc Food Sci Nutr 42(3): 450-456 https://doi.org/10.3746/jkfn.2013.42.3.450
  23. Lim JW, Moon JS, Kim HD, Na DJ, Son JY. 2004. Changes quality characteristics of Kimchi by storage containers. Korean J Food Nutr 17(1): 80-85
  24. Nam HG, Jang MS, Seo KC, Nam KH, Park HY. 2013. Changes in the taste compounds of Kimchi with seafood added during its fermentation. J Food Preserv 20(3): 404-418 https://doi.org/10.11002/kjfp.2013.20.3.404
  25. Noh JS, Seo HJ, Oh JH, Lee MJ, Kim MH, Cheigh HS, Song YO. 2007. Development of auto-aging system built in Kimchirefrigerator for optimal fermentation and storage of Korean cabbage Kimchi. Korean J Food Sci Technol 39(4): 432-437
  26. Oh YE, Kim SD. 1997. Changes in enzyme activities of salted Chinese cabbage and Kimchi during salting and fermentation. J Korean Soc Food Sci Nutr 26(3): 404-410
  27. Ohtsubo S, Asano S, Sato K, Matsumoto I. 2000. Enzymatic production of gamma-aminobutyric acid using rice (Oryza sativa) germ. Food Sci Technol Res 6(3): 208-211 https://doi.org/10.3136/fstr.6.208
  28. Park HJ, Lee CH. 2008. Food preservation. Korea University Press. Seoul. p 129
  29. Park KB, Oh SH. 2006. Isolation and characterization of Lactobacillus buchneri strains with high ${\gamma}$-aminobutyric acid producing capacity from naturally aged cheese. Food Sci Biotechnol 15(1): 86-90
  30. Park KW, Kim GH. 2012. Healthy functional vegetables and fruits.Sunjinmunhwasa. Goyang-si. pp 258-261
  31. Park SH, Lee JH. 2006. Consumer acceptance and sensory characteristics of Kimchi prepared with different kinds of subsidiary ingredients. Korean J Food Cookery Sci 22(3): 370-378
  32. Park SK, Cho YS, Park JR, Moon JS, Lee YS. 1995. Changes in the contents of sugar, organic acid, free amino acid and nucleic acid-related compounds during fermentation of leaf mustard-Kimchi. J Korean Soc Food Sci Nutr. 24(1): 48-53
  33. Samec D, Piljac-Zegarac J, Bogovic M, Habjanic K, Gruz J. 2011. Antioxidant potency of white (Brassica oleracea L. var. capitata) and Chinese (Brassica rapa L. var. pekinensis (lour.)) cabbage: The influence of development stage, cultivar choice and seed selection. Sci Hortic 128(2): 78-83 https://doi.org/10.1016/j.scienta.2011.01.009
  34. Seok JH, Park KB, Kim YH , Bae MO, Lee MK, Oh SH. 2008. Production and characterization of Kimchi with enhanced levels of ${\gamma}$-aminobutyric acid. Food Sci Biotechnol 17(5): 940-946
  35. Streeter JG, Thompson JF. 1972. In vivo and in vitro studies on ${\gamma}$-aminobutyric acid metabolism with the radish plant (Raphanus sativus, L.). Plant Physiol 49(4): 579-584 https://doi.org/10.1104/pp.49.4.579

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