Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Mycelial Production and Amylase Activity of Fungi for Brewing in Different Submerged Culture Conditions

액체배양에서의 양조용 곰팡이의 균체 생산 및 전분분해효소 활성

  • Noh, Jong-Min (Fermented Food Science Division, National Academy of Agricultural Science) ;
  • Choi, Ji-Ho (Fermented Food Science Division, National Academy of Agricultural Science) ;
  • Jung, Seok-Tae (Fermented Food Science Division, National Academy of Agricultural Science) ;
  • Yeo, Soo-Hwan (Fermented Food Science Division, National Academy of Agricultural Science) ;
  • Park, Jang-Woo (Dept. of Food and Biotechnology, Hankyong University) ;
  • Lee, Jin-Won (Dept. of Food and Biotechnology, Hankyong University) ;
  • Choi, Han-Seok (Fermented Food Science Division, National Academy of Agricultural Science)
  • 노종민 (국립농업과학원 발효식품과) ;
  • 최지호 (국립농업과학원 발효식품과) ;
  • 정석태 (국립농업과학원 발효식품과) ;
  • 여수환 (국립농업과학원 발효식품과) ;
  • 박장우 (한경대학교 식품생물공학) ;
  • 이진원 (한경대학교 식품생물공학) ;
  • 최한석 (국립농업과학원 발효식품과)
  • Received : 2013.11.25
  • Accepted : 2013.12.31
  • Published : 2013.12.31
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Abstract

In this study, twelve strains of brewing fungi were individually cultivated on wheat extract broth (WEB), potato dextrose broth (PDB) and malt extract broth (MEB) in order to determine the microorganism with good culture characteristics as well as with amylolytic activity. The strain cultured in PDB exhibited mycelia production from 12.6 g/L (Rhizopus oryzae KACC 45714) to a maximum of 48.0 g/L (Aspergillus oryzae KACC 46959), which was 2.3~9.2 times lower than that of the strain cultured in WEB and 1.7~14.6 times lower than that of the strain cultured in MEB. Accorfing the results, We found that the commercial strains of A. oryzae Suwon, CF1001 and CF1003 had a higher dry cell mass than the wild-type strains KACC 46421, 46423, 46424 and 46959. For Rhizopus sp., the acidity levels in WEB, PDB and MEB were 0.12~0.47%, 0.22~1.0% and 0.16~0.68% (equivalent lactic acid concentration) respectively. For A. oryzae, the acidity levels were 0.06~0.11%, 0.03~0.04% and 0.06~0.08% (equivalent lactic acid concentration), respectively. Amylase enzyme from Rhizopus delemar KACC 46419 exhibited an enzyme activity of 0.013 U and 0.019 U in WEB and MEB cultures, respectively. The enzyme activity of the amylase enzyme from A. oryzae was 0.019~0.037, 0.017~0.033 and 0.028~0.046 U in WEB, PDB and MEB cultures, respectively.

양조용 곰팡이 12균주에 대하여 WEB(wheat extract broth), PDB(potato dextrose broth), MEB(malt extract broth) 배지에서의 배양특성 및 전분분해 효소 활성을 조사하였다. PDB 배지에서의 균체 생산량은 1.26(R. oryzae KACC 45741)~4.80(A. oryzae KACC 46959) g/100 mL로 WEB, MEB 배지보다 각각 2.3~9.2배, 1.7~14.6배 낮았다. A. oryzae의 경우, 상업유통 균주인 Suwon, CF1001, CF1003 균주들이 야생 균주인 KACC 46421, KACC 46423, KACC 46424, KACC 46959보다 건조 균체량이 높았다. Rhizopus sp.의 산 생성량은 WEB, PDB, MEB 배지에서 각각 0.12~0.47, 0.22~1.0, 0.16~0.68%(as lactic acid)이었으며, A. oryzae는 0.06~0.11, 0.03~0.04, 0.06~0.08%(as citric acid) 범위이었다. 전분분해효소 활성은 Rhizopus sp.의 경우, R. delemer KACC 46419 균주만 WEB와 MEB에서 각각 0.013 U, 0.019 U의 활성을 보인 반면, A. oryzae의 효소 활성은 WEB, PDB, MEB 배지에서 각각 0.019~0.037, 0.017~0.033, 0.028~0.046 U 범위였다. 결론적으로 액체 종국용 배지는 균체 생산성이 비교적 우수하였고, 효소활성이 높았던 MEB 배지를 사용하는 것이 유리한 것으로 생각된다.

Keywords

References

  1. 과학기술부 (1998) 전통누룩과 전통주의 품질향상 및 산업화 기술연구. 농촌진흥청 작물시험장, 서울. pp 114-118.
  2. Cho HK, Seo WT, Lee JY, Cho KM (2012) Quality characteristics of cereal makgeolli rice nuruk prepared Rhizopus oryzae CCS01. J Korean Soc Food Sci Nutr 41: 1002-1008 https://doi.org/10.3746/jkfn.2012.41.7.1002
  3. Choi JS, Jung ST, Choi JH, Choi HS, Baek SY, Yes SH (2012) Quality characteristics of wheat nuruks by storage conditions of liquid starters using Rhizopus oryzae N174. Korean J Microbiol Biotechnol 40: 319-324. https://doi.org/10.4014/kjmb.1205.05029
  4. Huh CK, Lee JW, Kim YD (2012) Quality characteristics of rice wine according to the rice wine seed mash with lactic acid concentration. Korean J Food Preserv 19: 933-938. https://doi.org/10.11002/kjfp.2012.19.6.933
  5. Hong SJ, Lee WH, Park KB, Park KB, Sung JM (2001) Growth condition of Flammulina velutipes in liquid culture. Korean J Biotechnol Bioeng 16: 335-358.
  6. Jung DH (1983) Fermentation and microbiology engineering. Sunjinmunwhasa, Seoul. pp 228-275.
  7. Jo YH, Sung NK, Cung DH, Yun HD (1979) Microbiological studies on the rice makkulli. Korean J Appl Microbiol Bioeng 7: 217-223.
  8. Lee JS, Lee TS, Choi JY, Lee DS (1996) Volatile flavor components in mash of nonglutinous rice takju during fermentation. J Korean Soc Agric Chem Biotechnol 39: 249-254.
  9. Lee WK, Kim JR, Lee MW (1987) Studies on the changes in free amino acids and organic acids of takju prepared with different koji strains. J Korean Soc Agric Chem Biotechnol 30: 323-327.
  10. OH DS (2004) Improvement of Aspergillus oryzae by mutagenesis and optimization of its sporulation conditions. Woosuk University, Wanju. pp 21-41.
  11. Park CS, Lee TS (2002) Quality characteristics of takju prepared by wheat flour nuruks. Korean J Food Sci Technol 34: 296-302.
  12. Park JW, Lee KH, Lee CY (1995) Identification of filamentous molds isolated from Korean traditional nuruk and their amylolytic activities. Korean J Appl Microbiol Biotechnol 23: 737-746.
  13. Park S, Gong JW, Lee KS (2008) Calcium absorption and growth characteristics of Agrocybe cylindracea mycelia in submerged culture. Korean J Food Sci Technol 40: 419- 423.
  14. Seo WT, Cho HK, Lee JY, Kim B, Cho KM (2012) Quality characteristics of wheat-rice makgeolli by making of rice nuruk prepared by Rhizopus oryzae CCS01. Korean Journal of Microbiology 48: 147-155. https://doi.org/10.7845/kjm.2012.48.2.147
  15. Shin DH (2010) Globalization trends and prospect of Korean traditional fermented foods. Food Science and Industry 43: 69-82.
  16. Shon SK, Rho YH, Kim HJ, Bae SM (1990) Takju brewing of uncooked rice starch using Rhizopus koji. Korean J Appl Microbiol Biotech 18: 506-510.
  17. So MH (1999) Characteristics of a modified nuruk made by inoculation of traditional nuruk microorganisms. Korean J Food & Nutr 12: 219-225.
  18. So MH, Lee JW (1996) Takju brewing by combined use of Rhizopus japonicus-nuruk and Aspergilus oryzae-nuruk. J Korean Soc Food Nutr 25: 157-162.
  19. So MH, Lee YS (2010) Effects of culture conditions of Rhizopus sp. ZB9 on the production of organic acid during the preparation of rice koji. Korea J Food & Nutr 22: 644-649.
  20. Liao W, Liu Y, Frear C, Chen S (2007) A new approach of pellet formation of a filamentous fungus-Rhizopus oryzae. Bioresource Technology 98: 3415-3423. https://doi.org/10.1016/j.biortech.2006.10.028