Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Tolerance and Histological Responses to Formalin of Black Seabream Acanthopagrus schlegelii Juveniles

감성돔(Acanthopagrus schlegelii) 치어의 포르말린에 대한 내성 및 조직학적 반응

  • Myeong, Jeong-In (Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Min, Byung Hwa (Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Park, Mi Seon (Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Hwang, Hyung Kyu (Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Do, Jeung-Wan (Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Jeoung, Kyung Il (Department of Marine Bio-material and Aquaculture, Pukyong National University) ;
  • Chang, Young Jin (Department of Marine Bio-material and Aquaculture, Pukyong National University) ;
  • Jeong, Dal Sang (Department of Aquaculture, Korea National College of Agriculture and Fisheries)
  • 명정인 (국립수산과학원 전략양식연구소) ;
  • 민병화 (국립수산과학원 전략양식연구소) ;
  • 박미선 (국립수산과학원 전략양식연구소) ;
  • 황형규 (국립수산과학원 전략양식연구소) ;
  • 도정완 (국립수산과학원 전략양식연구소) ;
  • 정경일 (부경대학교 해양바이오신소재학과) ;
  • 장영진 (부경대학교 해양바이오신소재학과) ;
  • 정달상 (한국농수산대학교 수산양식학과)
  • Received : 2013.09.27
  • Accepted : 2013.12.16
  • Published : 2013.12.31
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Abstract

Black seabream juveniles Acanthopagrus schlegelii held at $20^{\circ}C$ were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24, 48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis, epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization, deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.

Keywords

References

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