The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Genotoxicological Safety Evaluation of the Solvent Extracts for Medicinal Herbs that are of Highly Domestic Spendings

국내 소비 주요 생약재의 유기용매 추출물에 대한 유전독성평가

  • Yoon, Won Ho (Dept. of Food Science & Biotechnology, Seoil University)
  • 윤원호 (서일대학교 식품생명과학과)
  • Received : 2013.09.02
  • Accepted : 2013.12.02
  • Published : 2013.12.31
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Abstract

This study is carried out to evaluate the genotoxicity of herbs (Angelica decursiva, Polypori umbellate, Astragalus membranaceua, Paeonia iactiflora, Glycyrrhiza uralensis, Cnidium officinale, Rehmannia glutinosa, Cyperus rotundus, Dioscorea batatas and Platycodi Radix) by using the Ames test. The Salmonella typhimurium reversion assay is being performed by using the Sal. typhimurium TA98, TA100, TA102, TA1535 and TA1537 as tester strains. Among 70% of ethanol extracts from 10 herbs, the number of revertant colonies is being increased in Astragalus membranaceua, Cnidium officinale and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of the metabolic activation. In case of dichloromethane and ethyl acetate fraction from 70% of ethanol extracts, the number of revertant colonies is increased in Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas in a dose-dependent manner, as compared with negative controls of metabolic activation. Such results indicate that Angelica decursiva, Astragalus membranaceua, Cnidium officinale, Rehmannia glutinosa and Dioscorea batatas all show genotoxic effects when being extracted with the solvent extractions such as 70% of ethanol, dichloromethane and ethyl acetate, and thus, they might be genotoxically- non-safe.

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References

  1. Bang SH, Han SS, Cho JH, Lee YW, Cho CK, Yoo HS. 2008. Study about herb-drug interaction for cancer patients. Korean J Orient Int Med 29:887-903
  2. Dias DA, Urban S, Roessner U. 2012. A historical overview of natural products in drug discovery. Metabolites 2:303-336 https://doi.org/10.3390/metabo2020303
  3. Jin YJ, Sung JS, Kim DG. 2009. The protective effects of Dioscoreae rhizoma on the exposure to UVA of MEF cells. J Orient Obstet Gynecol 22:36-50
  4. Joo GJ, Kim YM, Woo CJ, Lee OS, Kim JW, So JH, Kwak YY, Lee JJ, Kim JH, Rhee IK. 2005. Development of microbial inoculant using by-product of oriental herbal medicine. J Korean Soc Appl Biol Chem 48:201-206
  5. Kilani S, Ben AR. 2005. Investigation of extracts from (Tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ Toxicol Pharmacol 20:478-484 https://doi.org/10.1016/j.etap.2005.05.012
  6. Kim JM, Jeong HY, Park SW, Youn SS, Cho CS, Kim CJ. 2011. Review of natural materials in diabetic peripheral neuropathy. Korean J Oriental Physiology & Pathology 25:1056-1060
  7. Kim JS, Han SH, Jung BK, Park SJ, Kim HW, Chae H, Kwon YK, Kim BJ. 2010. Effects of the Korean herbal bathing candidates on allergic contact or wound induced dermatitis mice model. Korean J Oriental Physiology & Pathology 24: 484-489
  8. Lee IC, Kim BH, Kim SO, Kim MK. 2011. Skin safety evaluation of a Korean traditional prescription, Dansam-samul-tang and Samwhang-sasim-tang. Korean J Oriental Physiology & Pathology 25:212-216
  9. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutation Res 113:173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  10. Mortelmans K. 2000. The Ames Salmonella/microsome mutagenicity assay. Mutation Res 455:29-60 https://doi.org/10.1016/S0027-5107(00)00064-6
  11. Newman DJ, Cragg GM. 2007. Natural products as sources of new drugs over the last 25 years. J Nat Prod 70:461-477 https://doi.org/10.1021/np068054v
  12. Ryu JS, Han SK, Shin MS, Koo BC, Choi JH. Lee JW, Jo CR, Ham JS, Lee WK. 2008. Genotoxicological safety evaluation of the gamma-irradiated milk and ice cream using the Salmonella typhimurium reversion assay. J Biomedical Research 9:11-16
  13. Weon JB, Ma JY, Yang HJ, Ma CJ. 2011. Neuroprotective activity of fermented Oyaksungisan. Korean J Pharmacogn 42:22-26

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