International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지
DOI QR코드

DOI QR Code

Oral Microbiota Comparison between Healthy volunteers, Periodontitis patients and Oral cancer patients

  • Na, Hee Sam (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Kim, Seyeon (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Choi, Yoon Hee (Division of Hematology-Oncology, Department of Internal Medicine, Dongnam Institute of Radiology and Medical Science) ;
  • Lee, Ju-Yeon (Department of Periodontology, School of Dentistry, Pusan National University) ;
  • Chung, Jin (Department of Oral Microbiology, School of Dentistry, Pusan National University)
  • Received : 2013.12.03
  • Accepted : 2013.12.18
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The presence of distinct bacterial species is found to be dependent on age, diet, and disease. We compared the detection rate of several oral bacterial strains in a cohort of 36 subjects including healthy volunteers, periodontal patients, and oral cancer patients. Gargling samples were obtained from these subjects from which DNA was then extracted. Specific primers for 29 bacterial species were used for PCR detection. In the oral cancer patients, Capnocytophaga ochracea, Gemella morbillorum, and Streptococcus salivarius were detected more frequently compared with the healthy volunteers and periodontitis patients. Fusobacterium nucleatum/ polymorphym and Prevotella nigrescens were significantly less prevalent in oral cancer patients than the other groups. In periodontitis patients, Porphyromonas gingivalis and Treponema denticola were more frequently found compared with the healthy volunteers. In the healthy volunteer group, Peptostreptococcus anaerobius was more frequently found than the other groups. The detection rate of several oral bacterial species was thus found to differ between healthy volunteers, periodontitis patients and oral cancer patients.

Keywords

References

  1. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005; 43:5721-5732. https://doi.org/10.1128/JCM.43.11.5721-5732.2005
  2. Zaura E, Keijser BJ, Huse SM, Crielaard W. Defining the healthy "core microbiome" of oral microbial communities. BMC Microbiol 2009; 9:259. https://doi.org/10.1186/1471-2180-9-259
  3. Lovegrove JM. Dental plaque revisited: bacteria associated with periodontal disease. J N Z Soc Periodontol 2004:7-21.
  4. Wade WG. The oral microbiome in health and disease. Pharmacol Res 2013; 69:137-143. https://doi.org/10.1016/j.phrs.2012.11.006
  5. Parahitiyawa NB, Scully C, Leung WK, Yam WC, Jin LJ, Samaranayake LP. Exploring the oral bacterial flora: current status and future directions. Oral Dis 2010; 16:136-145. https://doi.org/10.1111/j.1601-0825.2009.01607.x
  6. Ruby J, Goldner M. Nature of symbiosis in oral disease. J Dent Res 2007; 86:8-11. https://doi.org/10.1177/154405910708600102
  7. Dye BA, Barker LK, Li X, Lewis BG, Beltran-Aguilar ED. Overview and quality assurance for the oral health component of the National Health and Nutrition Examination Survey (NHANES), 2005-08. J Public Health Dent 2011; 71:54-61. https://doi.org/10.1111/j.1752-7325.2010.00202.x
  8. Thornton-Evans G, Eke P, Wei L, Palmer A, Moeti R, Hutchins S, Borrell LN. Periodontitis Among Adults Aged >/=30 Years - United States, 2009-2010. MMWR Surveill Summ 2013; 62:129-135.
  9. Filoche SK, Cornford E, Gaudie W, Wong M, Heasman P, Thomson WM. Smoking, chronic periodontitis and smoking cessation support: reviewing the role of dental professionals. N Z Dent J 2010; 106:74-77.
  10. Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet 2005; 366:1809-1820. https://doi.org/10.1016/S0140-6736(05)67728-8
  11. Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer. Oral Oncol 2009; 45:309-316. https://doi.org/10.1016/j.oraloncology.2008.06.002
  12. Meurman JH. Oral microbiota and cancer. J Oral Microbiol 2010; 2.
  13. Boonanantanasarn K, Gill AL, Yap Y, Jayaprakash V, Sullivan MA, Gill SR. Enterococcus faecalis enhances cell proliferation through hydrogen peroxide-mediated epidermal growth factor receptor activation. Infect Immun 2012; 80:3545-3558. https://doi.org/10.1128/IAI.00479-12
  14. White DA, Tsakos G, Pitts NB, Fuller E, Douglas GV, Murray JJ, Steele JG. Adult Dental Health Survey 2009: common oral health conditions and their impact on the population. Br Dent J 2012; 213:567-572. https://doi.org/10.1038/sj.bdj.2012.1088
  15. Moore WE, Moore LV. The bacteria of periodontal diseases. Periodontol 2000 1994; 5:66-77. https://doi.org/10.1111/j.1600-0757.1994.tb00019.x
  16. Cortelli JR, Aquino DR, Cortelli SC, Fernandes CB, de Carvalho-Filho J, Franco GC, Costa FO, Kawai T. Etiological analysis of initial colonization of periodontal pathogens in oral cavity. J Clin Microbiol 2008; 46:1322-1329. https://doi.org/10.1128/JCM.02051-07
  17. Pushalkar S, Ji X, Li Y, Estilo C, Yegnanarayana R, Singh B, Li X, Saxena D. Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma. BMC Microbiol 2012; 12:144. https://doi.org/10.1186/1471-2180-12-144
  18. Pushalkar S, Mane SP, Ji X, Li Y, Evans C, Crasta OR, Morse D, Meagher R, Singh A, Saxena D. Microbial diversity in saliva of oral squamous cell carcinoma. FEMS Immunol Med Microbiol 2011; 61:269-277. https://doi.org/10.1111/j.1574-695X.2010.00773.x
  19. Hooper SJ, Crean SJ, Fardy MJ, Lewis MA, Spratt DA, Wade WG, Wilson MJ. A molecular analysis of the bacteria present within oral squamous cell carcinoma. J Med Microbiol 2007; 56:1651-1659. https://doi.org/10.1099/jmm.0.46918-0
  20. Shiga K, Tateda M, Saijo S, Hori T, Sato I, Tateno H, Matsuura K, Takasaka T, Miyagi T. Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis. Oncol Rep 2001; 8:245-248.
  21. Tateda M, Shiga K, Saijo S, Sone M, Hori T, Yokoyama J, Matsuura K, Takasaka T, Miyagi T. Streptococcus anginosus in head and neck squamous cell carcinoma: implication in carcinogenesis. Int J Mol Med 2000; 6:699-703.
  22. Sakamoto H, Naito H, Ohta Y, Tanakna R, Maeda N, Sasaki J, Nord CE. Isolation of bacteria from cervical lymph nodes in patients with oral cancer. Arch Oral Biol 1999; 44:789-793. https://doi.org/10.1016/S0003-9969(99)00079-5
  23. Ruoff KL. Streptococcus anginosus ("Streptococcus milleri"): the unrecognized pathogen. Clin Microbiol Rev 1988; 1:102-108. https://doi.org/10.1128/CMR.1.1.102
  24. Jacobs JA, Stobberingh EE. Hydrolytic enzymes of Streptococcus anginosus, Streptococcus constellatus and Streptococcus intermedius in relation to infection. Eur J Clin Microbiol Infect Dis 1995; 14:818-820. https://doi.org/10.1007/BF01691002
  25. Unsworth PF. Hyaluronidase production in Streptococcus milleri in relation to infection. J Clin Pathol 1989; 42:506-510. https://doi.org/10.1136/jcp.42.5.506
  26. Mager DL, Ximenez-Fyvie LA, Haffajee AD, Socransky SS. Distribution of selected bacterial species on intraoral surfaces. J Clin Periodontol 2003; 30:644-654. https://doi.org/10.1034/j.1600-051X.2003.00376.x
  27. Mager DL, Haffajee AD, Devlin PM, Norris CM, Posner MR, Goodson JM. The salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. J Transl Med 2005; 3:27. https://doi.org/10.1186/1479-5876-3-27
  28. Kobayashi N, Ishihara K, Sugihara N, Kusumoto M, Yakushiji M, Okuda K. Colonization pattern of periodontal bacteria in Japanese children and their mothers. J Periodontal Res 2008; 43:156-161. https://doi.org/10.1111/j.1600-0765.2007.01005.x
  29. Cogulu D, Uzel A, Oncag O, Eronat C. PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106:443-449. https://doi.org/10.1016/j.tripleo.2008.03.004
  30. Wahyuningsih R, Freisleben HJ, Sonntag HG, Schnitzler P. Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J Clin Microbiol 2000; 38:3016-3021.
  31. Gomes BP, Montagner F, Jacinto RC, Pinheiro ET, Zaia AA, Ferraz CC, Souza-Filho FJ. Gemella morbillorum in primary and secondary/persistent endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 105:519-525. https://doi.org/10.1016/j.tripleo.2007.10.005
  32. Ahmed S, Macfarlane GT, Fite A, McBain AJ, Gilbert P, Macfarlane S. Mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples. Appl Environ Microbiol 2007; 73:7435-7442. https://doi.org/10.1128/AEM.01143-07
  33. Yamaura M, Sato T, Echigo S, Takahashi N. Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction. Oral Microbiol Immunol 2005; 20:333-338. https://doi.org/10.1111/j.1399-302X.2005.00229.x
  34. Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fujiwara T. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis 2004; 48:195-199. https://doi.org/10.1016/j.diagmicrobio.2003.10.002
  35. Yano A, Kaneko N, Ida H, Yamaguchi T, Hanada N. Real-time PCR for quantification of Streptococcus mutans. FEMS Microbiol Lett 2002; 217:23-30.
  36. Igarashi E, Kamaguchi A, Fujita M, Miyakawa H, Nakazawa F. Identification of oral species of the genus Veillonella by polymerase chain reaction. Oral Microbiol Immunol 2009; 24:310-313. https://doi.org/10.1111/j.1399-302X.2009.00513.x
  37. Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol 1996; 11:266-273. https://doi.org/10.1111/j.1399-302X.1996.tb00180.x