Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Development of Gas Chromatography/Mass Spectrometry for the Determination of Essential Fatty Acids in Food Supplemental Oil Products

  • Ahn, Seonghee (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science) ;
  • Yim, Yoon-Hyung (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science) ;
  • Kim, Byungjoo (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science)
  • Received : 2013.12.05
  • Accepted : 2013.12.11
  • Published : 2013.12.30
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Abstract

A gas chromatography/mass spectrometric (GC/MS) method was developed as a candidate reference method for the accurate determination of essential fatty acids (linoleic acid, ${\alpha}$- and ${\gamma}$-linolenic acids) in food supplemental oil products. Samples were spiked with three internal standards (stearic acid-$d_{35}$, $^{13}C_{18}$-linoleic acid, and $^{13}C_{18}$-${\alpha}$-linolenic acid). Samples were then subject to saponification, derivatization for methylation, and extraction by organic solvent. For GC/MS measurement, an Agilent HP-88 column, designed for the separation of fatty acid methyl esters, was selected after comparing with other columns as it provided better separation for target analytes. Target analytes and internal standards were detected by selected ion monitoring of molecular ions of their methyl ester forms. The GC/MS method was applied for the measurement of three botanical oils in NIST SRM 3274 (borage oil, evening primrose oil, and flax oil), and measurement results agreed with the certified values. Measurement results for target analytes which have corresponding isotope-labeled analogues as internal standard were calculated based on isotope dilution mass spectrometry (IDMS) approach, and compared with results calculated by using the other two internal standards. Results from the IDMS approach and the typical internal standard approach were in good agreement within their measurement uncertainties. It proves that the developed GC/MS method can provide similar metrological quality with IDMS methods for the measurement of fatty acids in natural oil samples if a proper fatty acid is used as an internal standard.

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References

  1. Stransky, K. S.; Zarevucka, M.; Wimmer, Z. Food Chem. 2005, 92, 569. https://doi.org/10.1016/j.foodchem.2004.10.010
  2. Sanders, L.; Schantz, M.; Sharpless, K.; Wise, S. Lipid Technol. 2009, 21, 7. https://doi.org/10.1002/lite.200800080
  3. Christie, W. Industrial Crops Products 1999, 10, 73. https://doi.org/10.1016/S0926-6690(99)00013-8
  4. Traitler, H.; Winter, H.; Richli, U.; Ingenbleek, Y. Lipids 1984, 19, 923. https://doi.org/10.1007/BF02534727
  5. Brondz, I. Anal. Chim. Acta 2002, 465, 1. https://doi.org/10.1016/S0003-2670(01)01467-2
  6. Chen, S.-H.; Chuang, Y.-J. Anal. Chim. Acta 2002, 465, 145. https://doi.org/10.1016/S0003-2670(02)00095-8
  7. Laakso, P. J. AOCS, 1997, 74, 1291.
  8. Lacaze, J.-P. C. L.; Stobo, L. A.; Turrell, E. A.; Quilliam, M. A. J. Chromatography A 2007, 51, 1145.
  9. De Leenheer, A. P.; Thienpont, L. M. Mass Spectrom. Rev. 1992, 11, 249. https://doi.org/10.1002/mas.1280110402
  10. De Bievre, P. Anal. Proc. 1993, 30, 328. https://doi.org/10.1039/ap9933000328
  11. Shin, H.; Kim, B.; Lee, J. Food Chem. 2013, 138, 1109. https://doi.org/10.1016/j.foodchem.2012.11.046
  12. Lee, J.; Jang, E.-S.; Kim, B. Anal. Chim. Acta 2013, 787, 132. https://doi.org/10.1016/j.aca.2013.05.034
  13. Lim, Y. O.; Kim, B. J.; Ahn, S.; Kim, J. Food Chem. 2011, 126, 1393. https://doi.org/10.1016/j.foodchem.2010.11.118