Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지
DOI QR코드

DOI QR Code

Action Mechanism of Chamaecyparis obtusa Oil on Hair Growth

  • Park, Young-Ok (Department of Public Health, Graduate School, Keimyung University) ;
  • Kim, Su-Eun (Department of Skin Care, Yeungnam College of Science & Technology) ;
  • Kim, Young-Chul (Department of Public Health, Graduate School, Keimyung University)
  • Received : 2013.11.11
  • Accepted : 2013.12.17
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to examine the action mechanism of Chamaecyparis obtusa oil (CO) on hair growth in C57BL/6 mice. For alkaline phosphatase (ALP) and ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GT) activities in the skin tissue, at week 4, the 3% minoxidil (MXD) and 3% CO treatment groups showed an ALP activity that was significantly higher by 85% (p < 0.001) and 48% (p < 0.05) and an ${\gamma}$-GT activity that was significantly higher by 294% (p < 0.01) and 254% (p < 0.05) respectively, as compared to the saline (SA) treatment group. For insulin-like growth factor-1 (IGF-1) mRNA expression in the skin tissue, at week 4, the MXD and CO groups showed a significantly higher expression by 204% (p < 0.05) and 426% (p < 0.01) respectively, as compared to the SA group. At week 4, vascular endothelial growth factor (VEGF) expression in the MXD and CO groups showed a significantly higher expression by 74% and 96% (p < 0.05) respectively, however, epidermal growth factor (EGF) expression in the MXD and CO groups showed a significantly lower expression by 66% and 61% (p < 0.05) respectively, as compared to the SA group. Stem cell factor (SCF) expression in the MXD and CO groups was observed by immunohis-tochemistry as significant in a part of the bulge around the hair follicle and in a part of the basal layer of the epidermis. Taking all the results together, on the basis of effects on ALP and ${\gamma}$-GT activity, and the expression of IGF-1, VEGF and SCF, which are related to the promotion of hair growth, it can be concluded that CO induced a proliferation and division of hair follicle cells and maintained the anagen phase. Because EGF expression was decreased significantly, CO could delay the transition to the catagen phase.

Keywords

References

  1. Paus, R. and Cotsarelis, G. (1999) The biology of hair follicles. N. Engl. J. Med., 341, 491-497. https://doi.org/10.1056/NEJM199908123410706
  2. Mak, K.K. and Chan, S.Y. (2003) Epidermal growth factor as a biologic switch in hair growth cycle. J. Biol. Chem., 278, 26120-26126. https://doi.org/10.1074/jbc.M212082200
  3. Ruckert, R., Lindner, G., Bulfone-Paus, S. and Paus, R. (2000) High-dose proinflammatory cytokines induce apoptosis of hair bulb keratinocytes in vivo. Br. J. Dermatol., 143, 1036-1039. https://doi.org/10.1046/j.1365-2133.2000.03784.x
  4. Hibino, T. and Nishiyama, T. (2004) Role of TGF-${\beta}$2 in the human hair cycle. J. Dermatol. Sci., 35, 9-18. https://doi.org/10.1016/j.jdermsci.2003.12.003
  5. Krause, K. and Foitzik, K. (2006) Biology of the hair follicle: the basics. Semin. Cutaneous Med. Surg., 25, 2-10.
  6. Hamada, K. and Suzuki, K. (1996) Evaluation of biochemical indices as a hair cycle marker in C3H mice. Exp. Anim., 45, 251-256. https://doi.org/10.1538/expanim.45.251
  7. Park, Y.O., Kim, Y.C. and Chang, B.S. (2013) Hair growth effect of Chamaecyparis obtusa oil in C57BL/6 mice. J. Invest. Dermatol., 9, 87-95.
  8. Iida, M., Ihara, S. and Matsuzaki, T. (2007) Hair cycle-dependent change of alkaline phosphatase activity in the mesenchyme and epithelium and mouse vibrissal follicles. Dev. Growth Differ., 49, 185-195. https://doi.org/10.1111/j.1440-169X.2007.00907.x
  9. Hahnel, A.C., Rappolee, D.A., Millan, J.L., Manes, T., Ziomek, C.A., Theodosiou, N.G., Werb, Z., Pedersen, R.A. and Schultz, G.A. (1990) Two alkaline phosphatase genes are expressed during early development in the mouse embryo. Development, 110, 555-564.
  10. Handjiski, B.K., Eichmuller, S., Hofmann, U., Czarnetzki, B.M. and Paus, R. (1994) Alkaline phosphatase activity and localization during the murine hair cycle. Br. J. Dermatol., 131, 303-310. https://doi.org/10.1111/j.1365-2133.1994.tb08515.x
  11. Hamada, K. (1997) Evaluation of the effects of hair regrowth substances on elongation of anagen period and blockagen of anagen-catagen transformation by hair cycle marker. Fragrance J., 5, 36-39.
  12. Kawabe, T.T., Kubicek, M.F., Johnson, G.A. and Buhl, A.E. (1994) Use of gamma-glutamyl transpeptidase activity as a marker of hair cycle and anagen induction in mouse hair follicles. J. Invest. Dermatol., 103, 122-126. https://doi.org/10.1111/1523-1747.ep12391894
  13. Lee, K.H., Han, S.I., Park, G.H. and Kwon, Y.E. (2003) Evaluation of the herbal extract mixture for the effects of hair-regrowth compared to 3% minoxidil: elongation of anagen period on C3H mice. Yakhak Hoeji, 47, 14-19.
  14. Hue, J.J., Li, L.S., Lyu, H., Baek, I.J., You, J.M., Nam, S.Y., Yun, Y.W., Hwang, S.Y., Hong, J.T. and Lee, B.J. (2005) Effect of Hwanggumgung, a natural product, on hair growth promotion in C57BL/6 mice. Yakhak Hoeji, 49, 518-526.
  15. Su, H.Y., Palmer, B.R., Wellby, M.P., Bickerstaffe, R. and Hickford, J.G. (2001) Differential expression of a gene homologous to a G-protein gene in neonatal mouse skin during development of hair follicles. J. Dermatol. Sci., 25, 10-19. https://doi.org/10.1016/S0923-1811(00)00101-8
  16. Otomo, S. (2002) [Hair growth effect of minoxidil]. Nippon Yakurigaku Zasshi, 119, 167-174. https://doi.org/10.1254/fpj.119.167
  17. Schlake, T., Beibel, M., Weger, N. and Boehm, T. (2004) Major shifts in genomic activity accompany progression through different stages of the hair cycle. Gene Expression Patterns, 4, 141-152. https://doi.org/10.1016/j.modgep.2003.09.009
  18. Weger, N. and Schlake, T. (2005). IGF-I signalling controls the hair growth cycle and the differentiation of hair shafts. J. Invest. Dermatol., 125, 873-882. https://doi.org/10.1111/j.0022-202X.2005.23946.x
  19. Hibberts, N.A., Messenger, A.G. and Randall, V.A. (1996) Dermal papilla cells derived from beard hair follicles secrete more stem cell factor (SCF) in culture than scalp cells or dermal fibroblasts. Biochem. Biophys. Res. Commun., 222, 401-405. https://doi.org/10.1006/bbrc.1996.0756
  20. Randall, V.A., Jenner, T.J., Hibberts, N.A., De Oliveira, I.O. and Vafaee, T. (2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles. J. Endocrinol., 197, 11-23. https://doi.org/10.1677/JOE-07-0522
  21. Harada, N., Okajima, K., Narimatsu, N., Kurihara, H. and Nakagata, N. (2008) Effect of topical application of raspberry ketone on dermal production of insulin-like growth factor-1 in mice and on hair growth and skin elasticity in humans. Growth Horm. IGF Res., 18, 335-344. https://doi.org/10.1016/j.ghir.2008.01.005
  22. Philpott, M.P., Sanders, D.A. and Kealey, T. (1994) Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro. J. Invest. Dermatol., 102, 857-861. https://doi.org/10.1111/1523-1747.ep12382494
  23. Oh, S.J. (2007) The isolation and culture of DP cell and the study of hair-induction activity. Master's thesis, Seoul, pp. 34-37.
  24. Detmar, M. (1996) Molecular regulation of angiogenesis in the skin. J. Invest. Dermatol., 106, 207-208. https://doi.org/10.1111/1523-1747.ep12340457
  25. Philpott, M.P. and Kealey, T. (1994) The effects of EGF on the morphology and patterns of DNA synthesis in freshly isolated and maintained human hair follicles. J. Invest. Dermatol., 102, 186-191. https://doi.org/10.1111/1523-1747.ep12371760
  26. Kim, J.D., Hue, J.J., Kang, B.S., Kim, J.H., Nam, S.Y., Yun, Y.W., Kim, J.S., Jeong, J.H., Kim, Y.C. and Lee, B.J. (2009) Effects of herbal extracts on hair growth in an animal model of C3H/HeJ mice. Lab. Anim. Res., 25, 225-232.
  27. Botchkareva, N.V., Khlgatian, M., Longley, B.J., Botchkarev, V.A. and Gilchrest, B.A. (2001) SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit. FASEB J., 15, 645-658. https://doi.org/10.1096/fj.00-0368com
  28. Chung, Y. (2007) Growth factor for hair and skin treatment. United States Patent Application Publication, U.S., pp. 1-11.

Cited by

  1. Effects of Essential Oil from Hinoki Cypress, Chamaecyparis obtusa, on Physiology and Behavior of Flies vol.10, pp.12, 2015, https://doi.org/10.1371/journal.pone.0143450
  2. Biofilm Formation and Expression of Virulence Factors vol.18, pp.7, 2015, https://doi.org/10.1089/jmf.2014.3309
  3. In vivo evaluation of insect wax for hair growth potential vol.13, pp.2, 2018, https://doi.org/10.1371/journal.pone.0192612