Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Identification of host plant species of Balanophora fungosa var. indica from Phnom Bokor National Park of Cambodia using DNA barcoding technique

캄보디아 프놈보콜국립공원의 Balanophora fungosa var. indica의 숙주식물에 대한 DNA barcoding 기법을 통한 동정

  • Kim, Joo Hwan (Department of Biological Science, Daegu University) ;
  • Won, Hyosig (Department of Biological Science, Daegu University)
  • Received : 2013.10.07
  • Accepted : 2013.11.04
  • Published : 2013.12.30
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Abstract

During the floristic survey on Phnom Bokor National Park, Kampot, Cambodia, we encountered Balanophora fungosa var. indica, which is a tropical holoparasitic plant. To identify its host species, we collected host roots and trees nearby and tried to identify them using DNA barcoding approach. We applied plastid rbcL and matK gene regions as DNA barcode markers, and successfully amplified and sequenced the markers from 15 host roots and seven tree samples. Obtained host root sequences were identified as Primulaceae, Celastraceae, Myrtaceae, and Oleaceae, while trees nearby are Oleaceae, Myrtaceae, Sapindaceae, Rosaceae, Clusiaceae, Ericaceae, and Lauraceae. At genus level, host species are identified as Myrsine, Euonymus, Syzygium, and Olea, but failed in species discrimination. Myrsine (Primulaceae) and Olea (Oleaceae) are reported here as host species of B. fungosa var. indica for the first time. Further sampling and comparative work, and DNA barcoding will help recognize the biodiversity of the area and host species of Balanophora, together with their evolution.

캄보디아 캄폿주 프놈보콜국립공원에 대한 식물상 조사 중 열대성 전기생식물인 B. fungosa var. indica를 발견하였다. 이들의 숙주를 확인하기 위해 숙주의 뿌리와 더불어 주변에 위치한 목본 식물을 채집하였으며, 이들을 DNA barcoding 방법을 사용하여 동정하였다. DNA barcode 마커로는 엽록체 rbcL 및 matK 유전자 구간을 적용하였으며, 15개의 숙주 뿌리와 7개의 주변 목본식물로부터 성공적으로 PCR 증폭 및 염기서열을 확보하였다. 숙주 뿌리로부터 얻어진 숙주의 염기서열은 앵초과, 노박덩굴과, 도금양과, 그리고 물푸레나무과로 식별되었으며, 주변의 목본식물은 물푸레나무과, 도금양과, 무환자나무과, 장미과, 물레나물과, 철쭉과와 녹나무과였다. 속 수준에서 앵초과는 Myrsine, 노박덩굴과는 Euonymus, 도금양과는 Syzygium, 물푸레나무과는 Olea 등으로 각각 식별되었으나, 종 수준에서의 동정은 불가능하였다. 앵초과 Myrsine와 물푸레나무과 Olea는 본 연구를 통해 최초로 B. fungosa var. indica의 숙주로 확인되었다. 추가적인 채집 조사 및 비교 연구, DNA barcoding을 통해 해당 지역의 생물다양성과 Balanophora속 식물의 숙주 식물 및 진화에 대해 좀더 명확하게 확인이 가능할 것으로 판단된다.

Keywords

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