한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제21권6호
  • /
  • Pages.486-492
  • /
  • 2013
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
DOI QR코드

DOI QR Code

황기의 추출조건 및 유효성분의 HPLC 분석법 평가

Evaluation on Extraction Conditions and HPLC Analysis Method for Bioactive Compounds of Astragali Radix

  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 노형준 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최재훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박춘근 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최수임 ((주)와이디생명과학연구소) ;
  • 홍승재 (경희의료원) ;
  • 김승유 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • 투고 : 2013.10.23
  • 심사 : 2013.11.27
  • 발행 : 2013.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at $85^{\circ}C$ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as $27.27{\pm}2.27%$, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of $10.55{\pm}0.24%$. The quantitative determination methods of calycosin-7-O-${\beta}$-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column ($4.6{\times}250mm$, $5{\mu}m$) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of $0.8mLmin^{-1}$ and a detection wavelength of 230nm. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-${\beta}$-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as $1,700.3{\pm}30.4$ and $443.6{\pm}8.4{\mu}g-1$, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at $85^{\circ}C$ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

키워드

참고문헌

  1. Cho WCS and Leung KN. (2007). In vitro and in vivo anti-tumor effects of Astragalus membranaceus. Cancer Letters. 252:43-54. https://doi.org/10.1016/j.canlet.2006.12.001
  2. Choi S, Park SR and Heo TR. (2005). Inhibitory effect of Astragali radix on matrix degradation in human articular cartilage. Journal of Microbiology and Biotechnology. 15:1258-1266.
  3. Choi SI, Heo TR, Min BH, Cui JH, Choi BH and Park SR. (2007). Alleviation of osteoarthritis by calycosin-7-O-${\beta}$-Dglucopyranoside( CG) isolated from Astragali radix(AR) in rabbit osteoarthritis(OA) model. Osteoarthritis and Cartilage. 15:1086-1092. https://doi.org/10.1016/j.joca.2007.02.015
  4. Dong TTX, Ma XQ, Clarke C, Song ZH, Ji ZN, Lo CK and Tsim KWK. (2003). Phylogeny of Astragalus in China: Molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. Journal of Agricultural and Food Chemistry. 51:6709-6714. https://doi.org/10.1021/jf034278x
  5. Du XG, Zhao B, Li JY, Cao XH, Diao MK, Feng HB, Chen XB, Chen ZY and Zeng XY. (2012). Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice. International Immunopharmacology. 14:463-470. https://doi.org/10.1016/j.intimp.2012.09.006
  6. Kajimura K, Takagi Y, Ueba N, Yamasaki K, Sakagami Y, Yokoyama H and Yoneda K. (1996). Protective effect of Astragali radix by intraperitoneal injection against Japanese encephalitis virus infection in mice. Biological & Pharmaceutical Bulletin. 19:855-859. https://doi.org/10.1248/bpb.19.855
  7. Kim JH, Park SY, Lim HK, Park AY, Kim JS, Kang SS, Youm JR and Han SB. (2007). Quantitative evaluation of Astragali radix through the simultaneous determination of bioactive isoflavonoids and saponins by HPLC/UV and LCESI-MS/MS. Bulletin of the Korean Chemical Society. 28:1187-1194. https://doi.org/10.5012/bkcs.2007.28.7.1187
  8. Kim JS and Kim CS. (1997). A study on the constituents from the roots of Astragalus membranaceus Bunge (II). Korean Journal of Pharmacognosy. 28:75-79.
  9. Kim JS, Kim YT and Kim CS. (2002). A study on the constituents from the roots of Astragalus membranaceus Bunge (III). Korean Journal of Pharmacognosy. 31:109-111.
  10. Kim SH, Jun YM, Lim JJ, Kim SH, Chung IM and Kim EH. (2012). Variation of astragalosides contents in cultivated Astragalus membranaceus. Korean Journal of Medicinal Crop Science. 20:372-380. https://doi.org/10.7783/KJMCS.2012.20.5.372
  11. Kim SP, Kim SM, Ryu HS, Shin JC, Lee DG, Lee OJ, Lee JH and Kim JH. (2012a). The effects of Astraglai radix pharmacopuncture at CV12 on osteoporosis of senescence accelerated mice(SAM) P6. The Journal of Korean Acupuncture & Moxibustion Medicine Society. 29:59-71.
  12. Lee DY, Noh HJ, Choi J, Lee KH, Lee MH, Lee JH, Hong Y, Lee SE, Kim SY and Kim GS. (2013). Anti-Inflammatory cycloartane-type saponins of Astragalus membranaceus. Molecules. 18:3725-3732. https://doi.org/10.3390/molecules18043725
  13. Lee YM, Choi SI, Lee JW, Jung SM, Park SM and Heo TR. (2005). Isolation of hyaluronidase inhibitory component from the roots of Astraglus membranaceus Bunge(Astragali radix). Food Science and Biotechnology. 14:263-267.
  14. Lee YS, Han OK, Park CW, Suh SI, Shin SW, Yang CH, Jeon TW, Lee ES, Kim KJ, Kim SH, Yoo WK and Kim HJ. (2003). Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells. Journal of Ethnopharmacology. 84:193-198. https://doi.org/10.1016/S0378-8741(02)00298-2
  15. Lei H, Wang B, Li WP, Yang Y, Zhou AW and Chen MZ. (2003). Anti-aging effect of astragalosides and its mechanism of action. Acta Pharmacologica Sinica. 24:230-234.
  16. Liu J, Zhao ZZ and Chen HB. (2011). Review of Astragali radix. Chinese Herbal Medicines. 3:90-105.
  17. Lv YW, Hu W, Wang YL, Huang LF, He YB and Xie XZ. (2011). Identification and determination of flavonoids in Astragali radix by high performance liquid chromatography coupled with DAD and ESI-MS detection. Molecules. 16:2293-2303. https://doi.org/10.3390/molecules16032293
  18. Ministry of Agriculture, Food and Rural Affairs(MAFRA). (2013). Production report of industrial crops in 2012. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea. p.85.
  19. Ministry of Food and Drug Safety(MFDS). (2013). The Korean pharmacopoeia. Ministry of Food and Drug Safety. Osong, Korea. p.1228.
  20. Ministry of Food and Drug Safety(MFDS). (2013). The functional ingredients recognized status of health functional food. Ministry of Food and Drug Safety. Osong, Korea. p.5.
  21. Wang XJ and Feng P. (2000). Antioxidant activity of Qizhu tang. Acta Pharmacologica Sinica. 21:1141-1144.
  22. Zhang WJ, Hufnagl P, Binder BR and Wojta J. (2003). Antiinflammatory activity of astragaloside IV is mediated by inhibition of NF-kappa B activation and adhesion molecule expression. Thrombosis and Haemostasis. 90:904-914.
  23. Zhang YD, Wang YL and Shen JP. (1984). Effect of astragaloside IV on inflammatory and hypertension. Acta Pharmacologica Sinica. 19:333-337.

피인용 문헌

  1. Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus vol.23, pp.5, 2015, https://doi.org/10.7783/KJMCS.2015.23.5.357
  2. Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-β-d-glucoside and Ecliptasaponin A in IL-1β-Stimulated Chondrosarcoma Cell Model vol.23, pp.11, 2018, https://doi.org/10.3390/molecules23112807