Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative Activity and Tyrosinase Inhibition Effect of Ethanol Extract and Its Fractions from the Branch of Rhododendron schlippenbachii

철쭉 가지 에탄올 추출물 및 용매별 분획물의 항산화 활성과 Tyrosinase 저해 활성

  • Im, Do Youn (Division of Pre-service Teacher Training & General Studies, Kwangju Women's University) ;
  • Lee, Kyoung In (Biotechnology Industrialization Center, Dongshin University)
  • 임도연 (광주여자대학교 교양교직과정부) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Received : 2013.09.25
  • Accepted : 2013.10.29
  • Published : 2013.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the antioxidative activity and tyrosinase inhibitory activity of 50% ethanol extract and its fractions from the branch of Rhododendron schlippenbachii. In DPPH radical scavenging ability, butanol and ethyl acetate fractions showed 59.98% and 55.17% of relative activity compared with positive control (ascorbic acid), but the 50% ethanol extract showed relatively low activity. In nitric oxide (NO) scavenging ability, the ethyl acetate and butanol fractions showed 141.80% and 131.55% relative activity compared with ascorbic acid as used for positive control. On the other hand, tyrosinase inhibitory activity of the ethyl acetate and butanol fractions showed about twice higher activity than positive control (arbutin). It means that the ethyl acetate and butanol fractions from the extract of R. schlippenbachii branch has ability for used as effective radical scavenger and tyrosinase inhibitor.

Keywords

References

  1. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  2. Byun SH, Yang CH and Kim SC. (2005). Inhibitory effect of Scrophulariae Radix extract on TNF-α IL-1β, IL-6 and nitric oxide production in lipopolysaccharide activated Raw 264.7 cells. Korean Journal of Herbology. 20:7-16.
  3. Chung HT, Pae HO, Choi BM, Billiar TR and Kim YM. (2001). Nitric oxide as a bioregulator of apoptosis. Biochemical and Biophysical Research Communications. 282:1075-1079. https://doi.org/10.1006/bbrc.2001.4670
  4. Dewick PM. (2002). Medicinal natural products. Wiley & Sons. Chichester, England. p.149-151.
  5. Eo KY and Kwon OD. (2009). Rhododendron poisoning in sheep and goats. Journal of Veterinary Clinics. 26:344-347.
  6. Im DY and Lee KI. (2011a). Antioxidative, antibacterial activity and tyrosinase inhibitory activity of the extract and fractions from Taraxacum coreanum Nakai. Korean Journal of Medicinal Crop Science. 19:238-245. https://doi.org/10.7783/KJMCS.2011.19.4.238
  7. Im DY and Lee KI. (2011b). Nitric oxide production inhibitory and scavenging activity and tyrosinase inhibitory activity of extracts from Taraxacum officinale and Taraxacum coreanum. Korean Journal of Medicinal Crop Science. 19:362-367. https://doi.org/10.7783/KJMCS.2011.19.5.362
  8. Jeong SM, Lee SH, Lim JS, Yoon SY, Ryu S, Lee JW, Kim SW, Yoo IS and You YH. (2009). A case of systemic toxicity that occurred in an adult who intentionally ingested Rhododendron sclippenbashii. Journal of the Korean Society of Clinical Toxicology. 7:180-182.
  9. Jung SW, Lee NK, Kim SJ and Han DS. (1995). Screening of tyrosinase inhibitor from plants. Korean Journal of Food Science and Technology. 27:891-896.
  10. Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention: Mechanism of action. Journal of Nutrition. 3479S-3485S.
  11. Manach C, Williamson G, Morand C, Scalbert A and Remesy C. (2005). Bioavailability and bioefficacy of polyphenols in humans. American Journal of Clinical Nutrition. 81:230S-242S. https://doi.org/10.1093/ajcn/81.1.230S
  12. Marcocci L, Maguire JJ, Droy-Lefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764
  13. Moreno MIN, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Enthropharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  14. Oh YJ, Seo HR, Choi TM and Jung DS. (2010). Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science. 18:373-378.
  15. Otto F and Denis W. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
  16. Popescu R and Kopp B. (2013). The genus Rhododendron: An ethnopharmacological and toxicological review. Journal of Ethnopharmacology. 147:42-62. https://doi.org/10.1016/j.jep.2013.02.022
  17. Radi R, Beckman JS, Bush KM and Freeman BA. (1991). Peroxynitrite oxidation of sulfhydryls, the cytotoxic potential of superoxide and nitric oxide. Journal of Biological Chemistry. 266:4244-4250.
  18. Sancheti S, Sancheti S, Lee SH, Lee JE and Seo SY. (2011). Screening of Korean medicinal plant extracts for α-glucosidase inhibitory activities. Italian Journal of Pharmaceutical Research. 10:261-264.
  19. Shin KM, Park YM, Kim IT, Hong SP, Hong JP and Lee KT. (2003). In vitro antiinflammatory activity of amygdalin in murine macrophage Raw 264.7 cells. Korean Journal of Pharmacognosy. 34:223-227.
  20. Videla LA and Fermandez V. (1988). Biochemical aspects of cellular oxidative stress. Archivos De Biologa Y Medicina Experimentales. 21:85-92.

Cited by

  1. Anti-oxidant and Inhibitory Activity on NO Production of Extract and its Fractions from Rosa davurica Pall. Leaves vol.23, pp.1, 2015, https://doi.org/10.7783/KJMCS.2015.23.1.20
  2. Antioxidant, α-Glucosidase Inhibitory and Antimicrobial Activities of Extracts from Maesa japonica (Thunb.) vol.22, pp.4, 2014, https://doi.org/10.7783/KJMCS.2014.22.4.289
  3. Antioxidant and Antiproliferative Activity of Extracts from Water Chestnut (Trapa japonica Flerow) vol.24, pp.1, 2016, https://doi.org/10.7783/KJMCS.2016.24.1.14
  4. Bioactive Component Analysis and Antioxidant Activity in Fresh Sprout of New Kalopanax septemlobus Cultivar vol.104, pp.3, 2015, https://doi.org/10.14578/jkfs.2015.104.3.397