Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Bacterial Expression of Cytochrome $b_5$ Type III Pseudogene

  • Baek, Sun-Ah (Department of Biology, Keimyung University) ;
  • Kim, Su-Won (Department of Biology, Keimyung University) ;
  • Kim, Jong-Won (Department of Biology, Keimyung University) ;
  • Yoo, Min (Department of Biology, Keimyung University)
  • Received : 2012.01.26
  • Accepted : 2012.06.27
  • Published : 2012.09.30
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Abstract

Cytochrome $b_5$ is involved in the reduction of methemoglobin back to hemoglobin, thereby maintaining normal function of the blood to carry oxygen around. Congenital abnormal condition of this enzyme causes a rare disease called methemoglobinemia. At least 4 different retropseudogenes are reported so far for cytochrome $b_5$. However, type III pseudogene has attracted most attention because it contains open reading frame in its structure. Although there is no evidence yet if this pseudogene is actually expressed in the cell or the blood the possibility of its expression needs to be elucidated. We have isolated type III pseudogene by polymerase chain reaction and cloned into pGEX-4T-1 expression vector followed by SDS-PAGE. Protein was expressed and the size of the expressed protein was 28 kDa as expected in its genetic code. This result also shows that the protein is not harmful for the viability of the microorganism. This study may contribute to the genetic diagnosis of cardiac diseases, possibly caused by cytochrome $b_5$.

Keywords

References

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