KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparative Enantiomer Separation on Chiral Stationary Phases Derived from Chiral Crown Ether by HPLC

고성능 액체 크로마토그래피에서 키랄 크라운 에테르로부터 유도된 키랄 고정상을 이용한 광학분리의 비교

  • 황호 (조선대학교 약학대학 약학과) ;
  • 전소희 (조선대학교 약학대학 약학과) ;
  • 김지연 (조선대학교 식품의약학과) ;
  • 이원재 (조선대학교 약학대학 약학과)
  • Received : 2012.08.08
  • Accepted : 2012.08.25
  • Published : 2012.08.31
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Abstract

Comparative liquid chromatographic enantiomer separation of ${\alpha}$-amino acids, their esters and primary amino compounds was performed using two chiral stationary phases (CSPs) prepared by covalently bonding (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA) of the same chiral selector. In general, the separation factors and resolution factors for these analytes on CSP 1 were greater than on CSP 2, while these capacity factors on CSP 2 were quite greater than on CSP 1. Except for leucine methyl ester and phenylalanine methyl ester, the elution orders of all analytes including ${\alpha}$-amino ${\alpha}$-alkyl acids and phenylglycine alkyl esters on CSP 1 are identical to those on CSP 2. This study showed that different connecting structures for these two CSPs might influence their ability to resolve the analytes depending on their structures related to the chiral recognition mechanism.

Keywords

References

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