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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cytotoxic Effect of Zostera asiatica on Growth of Human Cancer Cells

왕거머리말의 인체 암세포에 대한 세포독성 효과

  • Hong, Joo-Wan (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Jung, Myung-Eun (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lee, Jung-Im (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Kim, Ho-Jun (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Chang, Jae-Soo (Department of Environmental Engineering, Korea Maritime University) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 홍주완 (한국해양대학교 해양환경.생명과학부) ;
  • 정명은 (한국해양대학교 해양환경.생명과학부) ;
  • 이정임 (한국해양대학교 해양환경.생명과학부) ;
  • 김호준 (한국해양대학교 해양환경.생명과학부) ;
  • 장재수 (한국해양대학교 환경공학과) ;
  • 서영완 (한국해양대학교 해양환경.생명과학부)
  • Received : 2012.08.10
  • Accepted : 2012.08.24
  • Published : 2012.08.31
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Abstract

Whole plants of Zostera asiatica were extracted twice with acetone/methylene chloride (A+M) and methanol (MeOH) in turn. The combined crude extracts were evaporated in vacuo and then the residue was partitioned between water and methylene chloride. The aqueous layer was fractionated into $H_2O$ and n-butanol and then the organic layer was also fractionated into 85% aq. MeOH and n-hexane, successively. The crude extracts and their solvent fractions were evaluated for their inhibitory effect on growth of human cancer cells AGS, HT-29, MCF-7, and HT-1080 cells by MTT reduction assay. Among samples tested, 85% aq. MeOH and n-hexane fractions showed strong cytotoxic effect against AGS, HT-29, and MCF-7 cells. On the other hand, for HT-1080 cell, 85% aq. MeOH fraction exhibited the strongest cytotoxic effect.

Keywords

References

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