Bulletin of the Korean Chemical Society

  • 제33권10호
  • /
  • Pages.3269-3273
  • /
  • 2012
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
권호 표지
DOI QR코드

DOI QR Code

High-Contrast Imaging of Biomolecular Interactions Using Liquid Crystals Supported on Roller Printed Protein Surfaces

  • 투고 : 2012.06.11
  • 심사 : 2012.07.10
  • 발행 : 2012.10.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, we report a new method for the high contrast imaging of biomolecular interactions at roller printed protein surfaces using thermotropic liquid crystals (LCs). Avidin was roller printed and covalently immobilized onto the obliquely deposited gold surface that was decorated with carboxylic acid-terminated self-assembled monolayers (SAMs). The optical response of LCs on the roller printed film of avidin contrasted sharply with that on the obliquely deposited gold surface. The binding of biotin-peroxidase to the roller printed avidin was then investigated on the obliquely deposited gold substrate. LCs exhibited a non-uniform and random orientation on the roller printed area decorated with the complex of avidin and biotin-peroxidase, while LCs displayed a uniform and planar orientation on the area without roller printed proteins. The orientational transition of LCs from uniform to non-uniform state was triggered by the erasion of nanometer-scale topographies on the roller printed surface after the binding of biotin-peroxidase to the surface-immobilized avidin. The specific binding events of protein-receptor interactions were also confirmed by atomic force microscopy and ellipsometry. These results demonstrate that the roller printing of proteins on obliquely deposited gold substrates could provide a high contrast signal for imaging biomolecular interactions using LC-based sensors.

키워드

참고문헌

  1. Gupta, V. K.; Skaife, J. J.; Dubrovsky T. B.; Abbott, N. L. Science 1998, 279, 2077. https://doi.org/10.1126/science.279.5359.2077
  2. Jang, C. H.; Tingey, M. L.; Korpi, N. L.; Wiepz, G. J.; Schiller, J. H.; Bertics, P. J.; Abbott, N. L. J. Am. Chem. Soc. 2005, 127, 8913.
  3. Tan, H.; Yang, S.; Shen, G.; Yu, R.; Wu, Z. Angew. Chem. Int. Ed. 2010, 122, 8790. https://doi.org/10.1002/ange.201004272
  4. Brake, J. M.; Daschner, M. K.; Luk, Y.-Y.; Abbott, N. L. Science 2003, 302, 2094. https://doi.org/10.1126/science.1091749
  5. Hartono, D.; Qin, W. J.; Yang, K.-L.; Yung, L.-Y. L. Biomaterials 2009, 30, 843. https://doi.org/10.1016/j.biomaterials.2008.10.037
  6. Fletcher, P. D. I.; Kang, N.-G.; Paunov, V. N. ChemPhysChem. 2009, 10, 3046. https://doi.org/10.1002/cphc.200900405
  7. Hu, Q. Z.; Jang, C. H. Analyst 2012, 137, 567. https://doi.org/10.1039/c1an15743d
  8. Hu, Q. Z.; Jang, C. H. ACS Appl. Mater. Interfaces 2012, 4, 1791. https://doi.org/10.1021/am300043d
  9. Hussain, A.; Pina A. S.; Roque, A. C. A. Biosens. Bioelectron. 2009, 25, 1. https://doi.org/10.1016/j.bios.2009.04.038
  10. Lin, I.-H.; Miller, D. S.; Bertics, P. J.; Murphy, C. J.; de Pablo J. J.; Abbott, N. L. Science 2011, 332, 1297. https://doi.org/10.1126/science.1195639
  11. Pandey, A.; Mann, M. Nature 2000, 405, 837. https://doi.org/10.1038/35015709
  12. Venien, A.; Levieux, D.; Dufour, E. J. Colloid Interface Sci. 2000, 223, 215. https://doi.org/10.1006/jcis.1999.6647
  13. Nielsen, U. B.; Cardone, M. H.; Sinskey, A. J.; MacBeath, G.; Sorger, P. K. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 9330. https://doi.org/10.1073/pnas.1633513100
  14. Park, S. J.; Jang, C. H. Nanotechnology 2010, 21, 425502. https://doi.org/10.1088/0957-4484/21/42/425502
  15. Kim, S. R.; Abbott, N. L. Adv. Mater. 2001, 13, 1445. https://doi.org/10.1002/1521-4095(200110)13:19<1445::AID-ADMA1445>3.0.CO;2-9
  16. Luk, Y. Y.; Tingey, M. L.; Hall, D. J.; Israel, B. A.; Murphy, C. J.; Bertics, P. J.; Abbott, N. L. Langmuir 2003, 19, 1671. https://doi.org/10.1021/la026152k
  17. Tingey, M. L.; Wilyana, S.; Snodgrass, E. J.; Abbott, N. L. Langmuir 2004, 20, 6818. https://doi.org/10.1021/la049728+
  18. Han, G. R.; Jang, C. H. Colloids and Surfaces B: Biointerfaces 2012, 94, 89. https://doi.org/10.1016/j.colsurfb.2012.01.023
  19. Park, M. K.; Jang, C. H. Bull. Korean Chem. Soc. 2010, 31, 1223. https://doi.org/10.5012/bkcs.2010.31.5.1223