KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Identification and Characteristics of Lactic Acid Bacteria Isolated from Shellfishes

패류로부터 젖산 세균의 분리 및 특성

  • Kang, Chang-Ho (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Jeong, Ho-Geon (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Koo, Ja-Ryong (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Jeon, Eun-Jin (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Kwak, Dae-Yung (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Hong, Chae-Hwan (Central Research Institute, Hyundai Motors) ;
  • Kim, Si-Hwan (Central Research Institute, Hyundai Motors) ;
  • Seo, Ji-Yeon (Central Research Institute, Hyundai Motors) ;
  • Han, Do-Suck (Central Research Institute, Hyundai Motors) ;
  • So, Jae-Seong (Department of Biological Engineering, College of Engineering, Inha University)
  • 강창호 (인하대학교 생물공학과) ;
  • 정호건 (인하대학교 생물공학과) ;
  • 구자룡 (인하대학교 생물공학과) ;
  • 전은진 (인하대학교 생물공학과) ;
  • 곽대영 (인하대학교 생물공학과) ;
  • 홍채환 (현대자동차 중앙연구소 기반기술연구팀) ;
  • 김시환 (현대자동차 중앙연구소 기반기술연구팀) ;
  • 서지연 (현대자동차 중앙연구소 기반기술연구팀) ;
  • 한도석 (현대자동차 중앙연구소 기반기술연구팀) ;
  • 소재성 (인하대학교 생물공학과)
  • Received : 2012.03.13
  • Accepted : 2012.05.17
  • Published : 2012.06.30
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Abstract

Lactic acid is an important product arising from the anaerobic fermentation by lactic acid bacteria (LAB). It is used in the pharmaceutical, cosmetic, chemical, and food industries as well as for biodegradable polymer and green solvent production. The poly lactic acid (PLA) is an important material for bio-plastic manufacturing process. For PLA production by new LAB, we screened LAB isolates from shellfish. A total of 28 LAB were isolated from various shellfishes. They were all Gram positive, oxidase and catalase negative. Based on API 50CHL kit, 7 strains among the 28 isolates were identified as Lactobacillus plantarum, 6 strains as Lactobacillus delbrueckii, 5 strains as Leuconostoc mesenteroides, 3 strains as Lactobacillus brevis, 2 strains as Lactococcus lactis, 1 strain as Lactobacillus salivarius, 1 strain as Lactobacillus paracasei, 1 strain as Lactobacillus pentosus, 1 strain as Lactobacillus fermentum and 1 strain as Pediococcus pentosaceu. Also, we examined the amount of total lactic acid produced by these new strains by HPLC analysis with Chiralpak MA column. One strain E-3 from Mytilus edulis was indentified as Lactobacillus plantarum and found to produce 20.0 g/L of D-form lactic acid from 20 g/L of dextrose. Further studies are underway to increase the D-lactic acid production by E-3.

Keywords

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