Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro propagation and multiple shoot induction of Rhodiola rosea L. by axillary bud culture

홍경천(Rhodiola rosea L.)의 액아배양을 통한 다신초 유도 및 기내 대량증식

  • Bae, Kee-Hwa (Hongcheon Institute of Medicinal Herb) ;
  • Ko, Myung-Suk (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Kim, Nam-Young (Department of Horticulture and Education, Korea National Arboretum) ;
  • Song, Jae-Mo (Nature Environment Research Office of Gangwon Province) ;
  • Song, Gwan-Pil (Jeju Environment Research Institute)
  • Received : 2012.06.15
  • Accepted : 2012.07.20
  • Published : 2012.06.30
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Abstract

An efficient in vitro propagation was established by using axillary bud explants of roseroot (Rhodiola rosea L.), which has been known as a medicinal plant in East Asia. Among various media tested, MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L $GA_3$ was found to be the best for multiple shoot formation (15 axillary shoots per axillary bud). In addition 1/2MS medium containing 50 g/L sucrose was best for shoot elongation (7.8 cm) and increasing total chlorophyll contents (8.64 mg/g) best. Maximum number of roots (17.7 roots per explant) was observed on the medium without plant growth regulators. Propagated plants were successfully acclimatized to ex vitro conditions, with a survival frequency of 97% after 12 weeks. Most rooted shoots grew well and produced viable seeds when grown in vitro culture conditions. Therefore, R. rosea can be effectively propagated in vitro by the system we developed in this study.

본 실험은 약리적 효능이 뛰어난 홍경천의 기관 (액아가 포함된 줄기 조직)배양을 통한 대량증식 방법의 일환으로 액아배양을 통한 다신초를 유도, 증식조건에 관해 BA와 GA3의 영향을 조사하였고 신초증식에 필요한 적정 sucrose의 농도를 확인하였다. 그 후 적절한 기내 발근 및 토양순화 조건을 탐색하여 최적의 홍경천의 생산 조건을 확립하였다. 다신초의 유도 및 증식은 $GA_3$와 BA를 혼합 처리가 효과적이었다. 신초증식에 효과적인 sucrose의 농도는 50 g/L가 첨가된 배지였으며, 평균 7 cm 이상의 신장을 보였다. 기내 발근은 옥신류 식물생장조절물질 무첨가 배지에서 가장 높은 발근길이와 개수를 나타냈다. 기내발근된 유식물체의 토양순화는 모래상을 제외하고는 모두 양호한 생장을 보였고, 특히 모래, 피트모스, 펄라이트가 모두 첨가된 배합토에서 가장 양호한 생장을 보였다. 이상의 결과는 수년전 국내에 도입하려다 실패한 약용식물인 홍경천의 기내배양을 통한 재배의 가능성을 제시하는 것이고 더 나아가 다양한 생물소재의 활용 방안의 기초재료로 활용될 것으로 보여진다.

Keywords

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