Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of Xanthomonas axonopodis pv. aurantifolii and Xanthomonas axonopodis pv. citrumelo by Triplex PCR

  • Yu, Sang-Mi (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Se-Won (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Seung-Don (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Park, Eun-Woo (Dept. of Applied Biology and Chemistry, Seoul National University) ;
  • Lee, Yong-Hoon (Division of Biotechnology, Chonbuk National University)
  • Received : 2011.11.09
  • Accepted : 2012.05.29
  • Published : 2012.06.30
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Abstract

Citrus bacterial canker is an economically important disease affecting citrus production in many citrusgrowing areas and several pathotypes have been recognized within the Xanthomonas pathogens causing canker. In view of the containment of the disease, accurate identification of the causal bacterium is important. In this study, triplex PCR method was developed by using the previously reported primers. Two groups of primer combination, such as, one group including primers 2/3, J-pth1/J-pth2 and XACF/XACR, and another group 2/3, J-pth1/J-pth2 and Xac01/Xac02, were suitable for the detection and differentiation of X. a. pv. citri $A^w$, X. a. pv. aurantifolii B and C, and X. a. pv. citrumelo E strains. Moreover, the primer combination of Xac01 and J-pth2 promised us to use as a specific primer set to detect X. a. pv. citrumelo E strain. The PCR methods developed in this study could be used for the rapid differentiation of Xanthomonas pathotypes of citrus.

Keywords

References

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