Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-wrinkle Compounds Isolated from the Seeds of Arctium lappa L.

우방자에서 분리한 주름개선 화합물

  • Hwang, Ju-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Hwang, Eun-Young (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Lee, Jung-Noh (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Ji-Young (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Ghang-Tai (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Lee, Kun-Kook (Coreana Cosmetics Co. Ltd. Songpa R&D Center) ;
  • Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
  • Received : 2012.06.27
  • Accepted : 2012.08.22
  • Published : 2012.08.30
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Abstract

This study was carried out to discover the skin wrinkle reducing components in the seeds of Arctium lappa. The isolation of a methylene chloride-soluble fraction of 70% ethanol extract from the seeds of Arctium lappa using a procollagen type-1 synthesis and MMP-1 activity resulted in the isolation and identification of four lignin compounds: arctiin, arctigenin, matairesinol, and diarctigenin. All structures were confirmed via NMR and MS spectroscopic data. To determine cell viability and procollagen type-1 synthesis, human dermal fibroblasts were treated with 10-100 ${\mu}M$. As a result, none of the four compounds showed cytotoxicity up to 50 ${\mu}M$. We also investigated their procollagen type-1 synthesis and MMP-1 inhibition activity and found that arctiin had the highest activity in terms of both procollagen synthesis and MMP-1 inhibition among all four compounds. Putting all the data together, we suggest that arctiin be used in cosmetics as an anti-wrinkle material.

본 연구에서는 우방자를 70% 에탄올로 추출하여 얻어진 추출물을 n-hexane, methylene chloride, ethyl acetate로 순차용매 분획하였다. 각 분획물에 대해 activity-guided isolation을 수행하여 활성물질의 분리 정제를 실시하였다. 활성을 나타내는 물질은 silica gel chromatography (230 mesh), sephadex LH 20, recrystallization method를 이용하여 분리하였다. 각 화합물의 화학구조는 NMR 스펙트럼 데이터 해석하였고 arctiin, arctigenin, diarctigenin, matairesinol 으로 동정하였다. 이들을 human dermal fibroblast HS68 세포에 처리하여 얻어진 상등액은 ELISA kit를 활용하여 procollagen type I 생합성과 MMP-1 저해활성을 측정하였다. 측정결과 procollagen type I 생합성과 MMP-1 저해활성 결과 모두 arctiin이 가장 우수한 결과를 보였다. 이와 같은 결과를 통해 우방자에서 분리한 리그난 화합물들을 이용하여 주름개선 소재로 개발할 수 있을 것으로 사료 된다.

Keywords

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