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Pseudomonas sp. 유래 녹두 부패병의 병 저항성 녹두 계통 검정

Evaluation of the Resistance of Mungbean Lines to Sprout Rot Caused by Pseudomonas species

  • 투고 : 2011.11.24
  • 심사 : 2012.06.26
  • 발행 : 2012.07.30

초록

녹두나물(숙주나물)은 국내뿐만 아니라 세계적으로도 널리 이용되고 있는 채소다. 그런데 녹두나물 재배를 하는 과정에서 발생되는 녹두나물 무름병은 녹두나물 생산량은 물론 품질을 심각하게 저하시킨다. 본 연구에서는 녹두나물 부패 조직으로부터 70계통의 병원균을 분리하였으며, 각 병원균의 병원성을 검정하였다. 그 가운데 강한 병원성을 가진 Pseudomonas 균류의 계통 YV-St-033를 확인하여 선발하였으며, 분리된 병원균계의 16S rRNA 유전자 염기서열을 분석하고 유전학적 유연관계를 분석하였다. YV-St-033는 P. mosselii, P. putita, P. fluorescens, P. entomophila, P. lecoglossicida 등의 종이 속한 그룹으로 확인이 되었으며, Pseudomonas mosselii R10 strain과 가장 높은 염기서열 identity (약 99%)를 보였다. 또한 YV-St-033 strain을 이용하여 영남대학교에서 보유하고 있는 녹두 유전자원들에 대해 녹두나물 무름병 저항성을 검정하였다. 3일간 배양한 녹두에 병원균을 접종하고 녹두의 생장율을 비교한 결과 YV148 line에서 높은 저항성이 확인되었으며, 그 외 녹두 계통에서도 부분적인 저항성을 나타내었다. 숙주나물 무름병에 저항성을 보인 YV 148 계통은 나물이 가늘고 연하고 생장율이 우수하여 앞으로 품종 육종의 좋은 재료로 활용될 수 있을 것으로 판단되었다.

Mungbean sprout rot is one of the most serious problems of the commercial mungbean sprout industry. In this study, 70 strains of mungbean sprout rot pathogens were isolated from rotten sprouts at different time intervals. The pathogenicity of the isolated pathogens was tested. The highly pathogenic strain (YV-St-033) was identified as Pseudomonas sp. by 16S rRNA gene sequencing. In phylogenetic analysis, the YV-St-033 strain was grouped with P. mosselii, P. putita, P. fluorescens, P. entomophila, and P. lecoglossicida. The results of the 16S rRNA gene sequence analysis revealed that the YV-St-033 strain shared the highest sequence identity (more than 99%) with the P. mosselii R10 strain. The mungbean lines of Yeungnam University germplasm were screened against the YV-St-033 strain. Based on the growth rate of the sprouts after 3 days of inoculation with the pathogen, the YV148 line was highly resistant to the pathogen. The remaining lines were either partially or fully infected. The highly resistant line YV 148 is suitable for future breeding programs due to their thin sprouts and fast growing nature.

키워드

참고문헌

  1. Devereux, R., He, S. H. and Doyle, C. L. 1990. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J. Bacteriol. 172, 3609-3619.
  2. Fry, N. K., Warwick, S. and Saunders, N. A. 1991. The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae. J. Gen. Microbiol. 137, 1215-1222. https://doi.org/10.1099/00221287-137-5-1215
  3. Han, K. S. and Lee, D. H. 1995. Identification and etiological characteristics of Anthracnose fungi isolated from soybean, small red bean and green bean. Korean J. Plant Pathol. 11, 30-38.
  4. Kang, S. G., Cha, H. W., Chang, M. U. and Park, E. H. 2003. Identification of differentially displayed genes of a Pseudomonas resistant soybean (Glycine max). Plant Pathol. J. 19, 239-247. https://doi.org/10.5423/PPJ.2003.19.5.239
  5. Kim, D. K., Lee, S. C., Kang, J. H. and Kim, H. K. 2003. Colletotrichum disease of mugbean sprout by Colletotrichum acutatum. Plant Pathol. J. 19, 203-204. https://doi.org/10.5423/PPJ.2003.19.4.203
  6. Kwon, S. H., Lim, K. H. and Kim, J. R. 1972. Studies on diversity of seed weight in Korean soybean land races and wild soybean. Korean J. Breed 4, 70-74.
  7. Kwon, S. H., Lee, Y. I. and Kim, J. R. 1981. Evaluation of important sprouting characteristics of edible soybean sprout cultivars. Korean J. Breed 13, 202-206.
  8. Lee, J. H., Han, K. S., Kim, T. H., Bae, D. W., Kim, D. K. and Kang, J. H. 2007. Effective heat treatment techniques for control of mungbean sprouts rot, incorporable into commercial mass production. Plant Pathol. J. 23, 174-179. https://doi.org/10.5423/PPJ.2007.23.3.174
  9. Lim, J. S., Do, K. S., Lee, D. S., Kang, S. G., Suh, S. G. and Park, E. H. 2008. Identifications of a sprout-rot pathogen Pseudomonas species SN239 and selection resistant soybean line. J. Life Sci. 18, 1771-1774. https://doi.org/10.5352/JLS.2008.18.12.1771
  10. Park, J. C., Song, W. Y. and Kim, H. M. 1996. Occurrence of bacterial soft rot of soybean sprout caused by Erwinia carotovora sub sp. carotovora. Korean J. Plant Pathol. 13, 13-17.
  11. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  12. Yun, S. C. and Kim, Y. H. 2003. Pathogenic bacteria causing rot in commercial soybean sprout cultivation. Kor. J. Crop Sci. 48, 113-119.