Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Rapid Detection of Cadmium-Resistant Plant Growth Promotory Rhizobacteria: A Perspective of ELISA and QCM-Based Immunosensor

  • Agrawal, Ruchi (Department of Biochemistry, CBSH, GB Pant University of Agriculture and Technology) ;
  • Satlewal, Alok (Department of Microbiology, CBSH, GB Pant University of Agriculture and Technology) ;
  • Chaudhary, Manav (Department of Biophysics, CBSH, GB Pant University of Agriculture and Technology) ;
  • Verma, Amit (Department of Biochemistry, CBSH, GB Pant University of Agriculture and Technology) ;
  • Singh, Rachna (Department of Biochemistry, CBSH, GB Pant University of Agriculture and Technology) ;
  • Verma, A.K. (Department of Biochemistry, CBSH, GB Pant University of Agriculture and Technology) ;
  • Kumar, Rajesh (Department of Microbiology, CBSH, GB Pant University of Agriculture and Technology) ;
  • Singh, K.P. (Department of Biophysics, CBSH, GB Pant University of Agriculture and Technology)
  • Received : 2011.08.23
  • Accepted : 2012.02.11
  • Published : 2012.06.28
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Abstract

Plant growth-promoting rhizobacteria (PGPR) pseudomonads have a large number of lipopolysaccharides on the cell surface, which induces immune responses. Cd-resistant PGPR prevalent at the Cd-affected sites under biophytostabilization was monitored. Transmissiom electron microscopy was used to the study the behavior of tolerance of PGPR to cadmium level and its effect on pseudomonad strains (Z9, S2, KNP2, CRPF, and NBRI). An immunosensor was developed by immobilizing antibody (anti-Z9 or anti-S2) against selected PGPR on a piezoelectric quartz crystal microbalance (QCM). Immunosensors were found to supplement the inherent specificity of antigen-antibody reactions with the high sensitivity of a physical transducer. On comparison of the efficiency of detection with ELISA, the spectrophotometric technique, the developed immunosensor was found to be more sensitive, fast, and reliable even after regeneration for several times. Thus, the immunosensor may be used for future detection of PGPR strains after automation of the screening process.

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