Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures

  • Rotaru, Amelia-Elena (Department of Microbiology, Max Planck Institute for Marine Microbiology) ;
  • Schauer, Regina (Department of Microbiology, Max Planck Institute for Marine Microbiology) ;
  • Probian, Christina (Department of Microbiology, Max Planck Institute for Marine Microbiology) ;
  • Mussmann, Marc (Department of Microbial Ecology, Max Planck Institute for Marine Microbiology) ;
  • Harder, Jens (Department of Microbiology, Max Planck Institute for Marine Microbiology)
  • Received : 2011.10.17
  • Accepted : 2011.12.05
  • Published : 2012.04.28
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Abstract

Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonene-degrading community.

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References

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