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Alcohol Fermentation of Uncooked Ground Rice with Ultrasonication Process

초음파를 이용한 무증자 분쇄 백미의 알코올 발효

  • Lee, You-Jin (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kang, Sung-Tae (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • 이유진 (서울과학기술대학교 식품공학과) ;
  • 강성태 (서울과학기술대학교 식품공학과)
  • Received : 2011.09.26
  • Accepted : 2011.12.09
  • Published : 2012.06.30

Abstract

This study was performed to evaluate the effects on saccharification and alcohol fermentation, according to the size of the ground rice (12, 20, 35 mesh) and ultra sonication process (15, 30, 60, 120 min). After saccharification of the ground rice samples for 3 h at $60^{\circ}C$, sugar content was observed to be high in the order of cooked ground rice (CGR) > ultrasonicated ground rice (UGR) > ground rice (GR), in all sizes of ground rice. Further, higher sugar content was obtained by increasing the time of ultrasonication process. Almost 90% saccharification of CGR ($11.5^{\circ}Bx$) could be reached up to $10.2^{\circ}Bx$ by ultrasonicating 35 mesh ground rice for 120 min. After alcohol fermentation on the 35 mesh saccharified-UGR for 4 days at $25^{\circ}C$, 16.7% alcohol concentration could be obtained, as high as of CGR (17.2%). After 4 days of alcohol fermentation, UGR showed a lower pH and a higher acidity (pH 4.06-4.17, 0.99-1.1%) than CGR (pH 4.27, 0.75%).

무증자 백미를 이용한 당화 및 알코올 발효를 최적화하기 위해 다양한 크기(12, 20, 35 mesh)로 분쇄된 백미를 초음파 처리시간(15, 30, 60, 120분)을 달리하여 시료들을 제조하고 당화와 알코올 발효를 수행하였다. 각 크기별로 분쇄된 백미시료를 $60^{\circ}C$에서 3시간 당화시킨 결과 모든 시료에서 가압증자 시료(CGR) > 초음파처리 시료(UGR) > 무증자 분쇄미(GR)의 순으로 높은 당도를 보여주였다. 분쇄백미의 크기에 무관하게 가압증자 시료(CGR)가 가장 높은 당도를 보여주었으며 무증자 분쇄미(GR)는 가장 낮은 당도를 나타내었다. 또한 무증자 분쇄미(GR)에 비하여 초음파처리 시료(UGR)가 높은 당도를 나타내었고 초음파 처리시간이 길수록 당도가 증가하였다. 35 mesh의 분쇄미를 120분 동안 초음파 처리함으로써 가압증자 시료(CGR, $11.5^{\circ}Bx$)의 약 90%에 해당하는 $10.2^{\circ}Bx$로 당화가 가능하였다. 또한 당화된 35 mesh의 초음파처리 시료(UGR)를 사용하여 $25^{\circ}C$에서 4일 동안 알코올 발효를 수행함으로써 가압증자 시료(CGR)의 알코올 함량 17.2%에 근접한 16.7%의 알코올 생산이 가능하였다. 알코올 발효 4일후의 무증자 초음파처리 시료군(UGR)의 pH는 가압증자 시료(CGR)와 비교하여 낮은 pH를 나타내었다. 또한 초음파처리 시료의 산도는 0.99-1.1%로서 가압증자 시료(CGR)의 산도 0.75%에 비하여 높은 산도를 보여주었다.

Keywords

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