한국잡초학회지 (Korean Journal of Weed Science)

한국잡초학회 & 한국잔디학회 (The Korean Society of Weed Science & The Turfgrass Society of Korea Archive)
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발효 당용액 생산자원으로서 담수조류 그물말의 유용성

Usefulness of Freshwater Alga Water-net (Hydrodictyon reticulatum) as Resources for Production of Fermentable Sugars

  • 김슬기 (한국화학연구원 바이오화학연구센터) ;
  • 황현진 (한국화학연구원 바이오화학연구센터) ;
  • 김재덕 (한국화학연구원 바이오화학연구센터) ;
  • 고은혜 (한국화학연구원 바이오화학연구센터) ;
  • 최정섭 (한국화학연구원 바이오화학연구센터) ;
  • 김진석 (한국화학연구원 바이오화학연구센터)
  • Kim, Seul-Ki (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Hwang, Hyun-Jin (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jae-Deog (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Ko, Eun-Hye (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Jung-Sup (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Seog (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 투고 : 2012.04.16
  • 심사 : 2012.06.01
  • 발행 : 2012.06.30
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초록

본 연구에서는 담수녹조류인 그물말(Hydrodictyon reticulatum, HR)을 산업바이오 자원으로서 활용하는 방안을 강구하고자 일차적으로 효소당화의 용이성을 검토하였다. HR에 대하여 효소당화를 2% 고형분 함량에서 동일조건으로 수행했을 때, 다른 종류의 바이오매스(Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover)보다 glucose 수득율이 가장 높았다. HR을 분말화하지 않아도 최적조건의 효소처리량에서 당화가 모두 이루어지며, HR 당화용액의 citrate buffer strength가 0.1mM까지 낮아도 당수득율에 큰 지장이 없었다. 또한 HR을 고온의 전처리없이 실온상태에서 바로 당화시켜도 $120^{\circ}C$ 처리에 비해 10% 미만의 당화율 감소만 나타내었다. 발효균주의 일반적인 생장적온인 $37^{\circ}C$ 또는 pH 6.5에서도 당화가 정상적으로 잘 일어나 당화/발효를 동시에 진행시킬 수 있는 바이오매스로의 특징을 보였다. 효소량을 기준량의 1/10정도 줄여도 최적조건의 70~80%에 해당하는 glucose 수득율을 나타내었다. 그리고 본 실험조건에서 HR의 고형분 함량 10%까지 당수득율이 떨어지지 않았고 15%이상되어야 감소하기 시작하여 고농도 당용액 생산에도 좋은 특성을 나타내었다. 이들의 제반 결과는 HR이 당화가 매우 쉽게 일어나는 특징을 가진 조류 바이오매스임을 나타내준다. 이러한 장점뿐만 아니라 수집하기가 매우 용이한 사상조류(flilamentous algae)이기 때문에 다른 종류의 조류 바이오매스에 비해 바이오화학제품 생산을 위한 원료로서 향후 이용가치가 매우 높을 것으로 판단된다.

To investigate the usefulness of freshwater alga Water-net (Hydrodictyon reticulatum, HR) as resources for production of fermentable sugars, the easiness of enzymatic saccharification was evaluated at first. When 6 plant materials (HR, Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover) were enzymatically hydrolyzed with 2% solid loading at the same condition, HR showed the highest ratio of saccharification based on glucose production. No milled HR was also completely saccharified at the amounts of optimal enzyme mixture. Glucose yield was not changed though the citrate buffer strength for saccharification was decreased from 0.1 M to 0.1 mM. Only about 10% yield reduction was observed compared to that of $120^{\circ}C$ treatment when HR was enzymatically hydrolyzed at room temperature. The saccharification was normally occurred at $37^{\circ}C$ and pH 6.5 which is general growth condition of fermentable microrganisms, suggesting that HR have a biomass characteristics applicable for the simultaneous saccharification and fermentation. The saccharification was occurred by more than 70~80% of one of the best condition although the supplied enzyme amounts was reduced to 1/10 volume. And the glucose yield by enzymatic hydrolysis was not decreased by 10% HR solid loading and began to decrease at more than 15% solid contents. Above these results show that HR is an interesting algal biomass which is relatively easy to be saccharified by hydrolyzing enzymes. In addition, HR is a flilamentous alga and very easy to be collected. Therefore, HR seems to be an useful and valuable resources in the economical production of fermentable sugars for manufacture of bio-chemical products.

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피인용 문헌

  1. Hydrolysis Methods for the Efficient Manufacture of Sugar Solutions from the Freshwater Alga Water-net (Hydrodictyon reticulatum) vol.2, pp.2, 2013, https://doi.org/10.5660/WTS.2013.2.2.176