Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Prevalence of Microbiological Hazard on Nursery School Children's Hands and Effect of Hand Washing Education

어린이집 유아 손의 미생물학적 위해 평가 및 손 씻기 교육의 효과

  • Kim, Jung-Beom (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Hur, Eun-Seon (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Kang, Suk-Ho (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Kim, Dae-Hwan (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Do, Young-Sook (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Park, Po-Hyun (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Park, Yong-Bae (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Yoon, Mi-Hye (Health Research Department, Gyeonggi-do Institute of Health & Environment) ;
  • Lee, Jong-Bok (Health Research Department, Gyeonggi-do Institute of Health & Environment)
  • 김중범 (경기도보건환경연구원 보건연구부) ;
  • 허은선 (경기도보건환경연구원 보건연구부) ;
  • 강석호 (경기도보건환경연구원 보건연구부) ;
  • 김대환 (경기도보건환경연구원 보건연구부) ;
  • 도영숙 (경기도보건환경연구원 보건연구부) ;
  • 박포현 (경기도보건환경연구원 보건연구부) ;
  • 박용배 (경기도보건환경연구원 보건연구부) ;
  • 윤미혜 (경기도보건환경연구원 보건연구부) ;
  • 이정복 (경기도보건환경연구원 보건연구부)
  • Received : 2011.09.17
  • Accepted : 2011.11.10
  • Published : 2012.03.31
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Abstract

This study was conducted to evaluate the microbiological hazard on nursery school children's hands and to investigate the reduction effect of hand washing education. A total of 59 nursery school children's hands were tested. The average number of total aerobic bacteria was $3.72{\pm}0.38log\;CFU/hand$. Five children's hands(2 male and 3 female) were positive(14.3%) for the coliform bacteria. These results showed that hand washing education are required repetitively. Among the pathogenic bacteria tested in this study, $Staphylococcus$ aureus and $Bacillus$ $cereus$ were detected in 9(25.7%) and 16(45.7%) out of 35 their hands, respectively. Twelve out of sixteen $B.$ $cereus$ isolates(70.0%) produced enterotoxin. The results indicate that the hand hygiene of nursery school children needs to be improved. Comparing before and after hand washing in educated and non-educated group, the reduction effect of total aerobic bacteria on their hands was 0.42 and 0.60 log CFU/hand, respectively. The educated group showed 0.18 log CFU/hand higher reduction effect than non-educated group but microorganism did not eliminate perfectly. From the results, using a hand sanitizer after washing with soap and the continuous hand washing education are required to control the contaminated bacteria on nursery school children's hands.

어린이집 유아 손의 미생물 오염도를 분석하고 손 씻기 교육의 미생물 저감화 효과를 실험하여 어린이집 유아 손의 위생 안전성을 확보하고자 어린이집 유아 59명을 대상으로 실험하였다. 일반세균수의 경우 평균 $3.72{\pm}0.38log\;CFU/hand$로 검출되었고, 위생지표미생물인 대장균군의 경우 남자어린이 18명 중 2명(11.1%)과 여자어린이 17명 중 3명(17.6%), 총 35명 중 5명(14.3%)에서 검출되어 어린이들에 대한 반복적인 손 씻기 지도가 필요한 것으로 판단되었다. $Staph.$ $aureus$는 35명 중 9명(25.7%), $B.$ $cereus$는 35명 중 16명(45.7%)에서 검출되었고 남자어린이의 손이 식중독 미생물 오염에 가장 취약한 것으로 나타났다. $Staph.$ $aureus$ 장독소는 9균주 중 $Staph.$ $aureus$ Hand-1 한 균주에서만 SEA 장독소가 검출되었으나 HBL과 NHE 장독소 중 한 가지 이상의 장독소를 생산하는 $B.$ $cereus$는 총 12균주(70.0%)로 나타나 어린이 손에 의한 교차오염 위험성이 상존한다고 판단되었다. 손 씻기 교육의 미생물 저감화 효과를 실험한 결과 교육을 실시한 그룹이 0.18 log CFU/hand 일반세균수가 더 저감화가 되었으나 저감화 정도가 매우 미약하여 손에 부착되어 있는 세균을 완전히 제거하기 위해서는 손 씻기와 손 소독을 병행하며 지속적인 손 씻기 교육을 실시하여야할 것으로 판단되었다.

Keywords

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