Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Probiotic Potential of Indigenous Bacillus sp. BCNU 9028 Isolated from Meju

메주로부터 분리한 토착 Bacillus sp. BCNU 9028의 프로바이오틱스로서 이용 가능성

  • Shin, Hwa-Jin (Department of Biology, Changwon National University) ;
  • Bang, Ji-Hun (Department of Biology, Changwon National University) ;
  • Choi, Hye-Jung (Department of Biology and interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Ahn, Cheol-Soo (Cho-A Pharm. Co, LTD.) ;
  • Jeong, Young-Kee (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • Received : 2012.02.29
  • Accepted : 2012.05.17
  • Published : 2012.05.30
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Abstract

Spore-forming bacteria are being used as probiotic supplements for human and animal use, due to their low pH stability and ability to survive the gastric barrier. In this study, the BCNU 9028 strain was screened from meju, a Korean fermented soybean food starter. Biochemical and physiological characteristics, as well as 16S rDNA sequence analyses, indicate that this strain belongs to the genus $Bacillus$. $Bacillus$ sp. BCNU 9028 showed a 92% survivability at pH 2.5 and could also withstand 0.3% ox bile. Furthermore, it was postulated that $Bacillus$ sp. BCNU 9028 could prevent biofilm formation and adherence of food-borne pathogens such as $Listeria$ $monocytogenes$, $S.$ $aureus$ and $E.$ $coli$ on the basis of its autoaggregation and coaggregation capacity with food-borne pathogens. It was shown that BCNU 9028 has good abilities to adhere to the intestinal tract from its hydrophobic character (63.3%). The $Bacillus$ sp. BCNU 9028 strain especially elicited antibacterial activity against both Gram-positive and -negative pathogens. These findings suggested that the $Bacillus$ sp. BCNU 9028 strain could be used as a potential probiotic.

포자형성균은 사람과 동물용의 프로바이오틱 제제로서 사용되어져 왔다. 포자형성균의 낮은 pH에 대한 안정성과 위내 생육저해 환경에 생존능은 프로바이오틱 제제로서 매력적이다. 본 연구는, 한국 전통 대두 발효 식품의 종균인 BCNU 9028균주를 메주로부터 분리하였다. 생리학적, 생화학적 특성과 16S 리보좀 DNA 염기서열 분석 결과 BCNU 9028균주는 $Bacillus$에 속하는 것을 확인하였다. $Bacillus$ sp. BCNU 9028은 pH 2.5에서 92%의 생존률을 보였으며, 0.3% 담즙산에서도 저항성을 나타냈다. 그리고, 식품 병원성균과의 응집정도와 자가결합능에 의해 $Bacillus$ sp. BCNU 9028는 $Listeria$ $monocytogenes$, $S.$ $aureus$ 및 $E.$ $coli$와 같은 식품 병원성균의 생물막형성과 부착을 저해할 수 있는 것을 확인하였다. BCNU 9028의 소수성 특성(63.3%)은 장관내 부착능이 우수할 것으로 나타났다. 특히, $Bacillus$ sp. BCNU 9028 균주는 그람양성 및 그람음성 병원성균 모두에 항균력을 갖는 것을 확인하였다. 이상의 결과로 $Bacillus$ sp. BCNU 9028균주가 프로바이오틱스로서 이용가능성이 있음을 제시하였다.

Keywords

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