Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Prevalence and Distribution of the P and G Genotypes of a Group A Rotavirus Detected in Acute Gastroenteritis Patients from Incheon

인천지역 급성 설사환자의 group A rotavirus 감염 실태 및 P와 G 유전자형 분포

  • Choi, Hye-Jin (Incheon Research Institute of Public Health and Environment) ;
  • Oh, Bo-Young (Incheon Research Institute of Public Health and Environment) ;
  • Lee, Mi-Yeon (Incheon Research Institute of Public Health and Environment) ;
  • Koh, Yeon-Ja (Incheon Research Institute of Public Health and Environment) ;
  • Gong, Young-Woo (Incheon Research Institute of Public Health and Environment) ;
  • Hur, Myung-Je (Incheon Research Institute of Public Health and Environment) ;
  • Lee, Jea-Mann (Incheon Research Institute of Public Health and Environment) ;
  • Kim, Young-Hee (Incheon Research Institute of Public Health and Environment) ;
  • Jeong, Hye-Sook (Korea Center for Disease Control & Prevention) ;
  • Cheon, Doo-Sung (Korea Center for Disease Control & Prevention)
  • Received : 2012.02.08
  • Accepted : 2012.03.22
  • Published : 2012.05.30
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Abstract

Rotavirus is the main cause of severe diarrhea in infants and young children of the world. However, the frequency of genetic alterations makes it hard to control the prophylaxis. Therefore, continuous monitoring of the rotavirus's genetic change is inevitable to prevent disease prevalence and is useful in inventing an efficient vaccine. From January 2005 to December 2010, we investigated 11,607 stool samples of acute gastroenteritis patients in the Incheon metropolitan area. About 13.18% (1,530 stool samples) of all samples had a positive reaction against rotavirus using an antigen capture enzyme-linked immunosorbent assay (ELISA). Then, the 160 stool samples were searched for subtypes of group A rotavirus by using a reverse transcription polymerase chain reaction (RT-PCR) and a nested multiplex RCR. In P sub-typing, P8 (56.3%) was an extremely prevalent genotype, followed by P6 (21.3%), and P1A (10.0%). G1 (39.4%) was most widespread in the G subtype, followed by G4 (25.0%) and G3 (18.8%). G1P8 (35.5%) was the most common G and P subtype combination, followed by G4P6 (19.3%) and G3P8 (13.1%). These results might be useful data for understanding the epidemiological status of group A-rotavirus dispersion in the Incheon metropolitan area.

인천지역 병 의원에 내원한 급성위장염환자의 대변 검체 총 11,607건을 효소 면역법(ELISA)을 이용하여 group A rotavirus의 계절별 발생 양상을 조사한 결과 2005년부터 2010년간 인천지역에서는 1~2월이 가장 많이 발생하였으나, 기간별로 차이가 존재했다. 또, group A rotavirus 양성 검체 160건에 유전자 분석 결과 VP4는 P8형, VP7는 G1형, G와 P 조합형으로는 G1P8이 가장 많았다. 그러나 이전의 연구와 비교했을 때 연구 지역과 기간에 따라 혈청형과 유전형의 변화가 빈번하였으므로 효율적인 방역을 위해 지속적인 모니터링이 필요하다고 사려된다. 또 이번 연구는 현재 유통 중인 rotavirus 백신의 효율적인 사용의 기초 자료가 될 것이다.

Keywords

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