Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
권호 표지

Potential for Application of Microhistological Analysis in Diet Plants of Korean Water Deer($Hydropotes$ $inermis$)

한국에 서식하는 고라니($Hydropotes$ $inermis$) 먹이식물의 미세조직분석법 적용 가능성 연구

  • Kim, Eui-Kyeong (Policy and Ecosystem Research Division, National Park Research Institute, Korea National Park) ;
  • Park, Yung-Chul (Department of Forest Environment Protection, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Kim, Won-Myeong (National Ecological Institute, Ministry of Environment) ;
  • Kim, Jong-Kuk (Department of Forest Environment Protection, College of Forest and Environmental Sciences, Kangwon National University)
  • 김의경 (국립공원관리공단 국립공원연구원 정책.조사연구부) ;
  • 박영철 (강원대학교 산림환경보호학과) ;
  • 김원명 (환경부 국립생태원 건립추진기획단) ;
  • 김종국 (강원대학교 산림환경보호학과)
  • Received : 2011.11.04
  • Accepted : 2012.04.03
  • Published : 2012.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to evaluate the application of microhistological analysis to collect information on food materials of korean water deer from september to October 2006. For microhistological analysis we extracted type-materials of 4 plant species ($Ipomoea$ $batatas$, $Rubus$ $coreanus$, $Glycine$ $max$, and $Pueraria$ $thunbergiana$) from fecal pellets of korean water deer ($Hydropotes$ $inermis$) and then made preparations of the fecal materials for microscopic analysis. The fecal preparations were compared with reference preparation under the microscope. In microscopic analysis of fecal preparations, we could identify the four plant species by the followings: tip shape and morphology of trichomes, epidermal cell, morphological composition and shape of stomatal apparatus, arrangement of long-cell, and distribution of crystals in cells. According to the comparisons of the preparations, the morphology of trichome, epidermal cell and stomata are similar between both preparations of the fecal pellets and the references, respectively. This methodology will contribute to understanding feeding behavior and behavioural ecology by climate change of other hervibores as well as korean water deers.

본 연구는 2006년 9월부터 10월까지 고라니가 주요하게 섭식하는 먹이식물에 대한 정보를 얻는데 있어 배설물에 포함된 식물세포의 미세조직분석 방법의 적용 가능성에 대해 조사하였다. 고라니 먹이식물의 미세조직분석을 위해 배설물로부터 고구마, 딸기, 콩, 칡 등 4가지 식물의 배설물 식물세포를 준비한 후 이들의 세포를 대조식물세포의 세포와 비교하여 검증하였다. 배설물 식물세포의 4가지 식물종의 세포는 모용의 기저 세포의 형태와 선단의 모양, 표피세포의 형태, 기공복합체의 구성 및 세포 모양, 장세포의 나열 형태와 세포에 포함되어 있는 결정체의 유무로 식물의 동정이 가능하였다. 배설물 식물세포와 대조식물세포를 비교 분석한 결과 배설물에서 나온 식물의 세포와 대조 식물세포에서 발견된 식물의 세포는 모용, 표피세포, 기공, 장세포가 모두 동일한 형태를 지니고 있었다. 이러한 방법을 적용하여 고라니의 섭식 식물종과 연관된 세포 도감을 작성한다면 향후 고라니 및 유사 초식동물들의 식이습성을 및 기후변화에 따른 행동생태 연구에 유용할 것이다.

Keywords

References

  1. Butzler, W.(1990) Grzimek's encyclopedia mammals. Vol. 5. McGraw-Hill Publishing Company, New York.
  2. Carriere, S.(2002) Photographic Key for the Microhistological Idntification of some Arctic Vascular Plants. Department of Resources, Wildlife and Economic Development, Government of the Northwest Territories, North America 55:247-268.
  3. Cooke, A. and L. Farrell(1998) Chinese water deer. The mammal society, London and the British Deer Society, Fordingbridge, pp. 1-32.
  4. Flinder, J.T. and R.M. Hansen(1972) Diet and habitats of jack rabbits in northeastern Colorado State Univ., Fort Collins, Co.
  5. Giannoni, S.M., C.E. Borghi, M. Dacar and C.M. Campos(2005) Main food categories in sigmodontine rodents in the Monte(ARGENTINA). Mastozoologia Neotropical 12(2):181-187.
  6. Gillen, R.L. and W.T. Kenneth(1993) The constituent differential method for determining live dead herbage. J. Range Management. 46: 142-147. https://doi.org/10.2307/4002271
  7. Guo, G. and E. Zhang(2005) Diet of the chinese water deer (Hydropotes inermis) in Zhoushan Archipelago, China. Acta Theriologica Sinica. 25(2): 122-130.
  8. Harris, R.B. and J.W. Duckworth(2008) Hydropotes inermis. In: IUCN 2010. IUCN Red List of Threatened Species. Version 2010.4..
  9. Heintzelmann, C.E., Jr. Heintzelmann and R.A. Howard(1948) The comparative morphology of the Icacinaceae. V. The pubescence and the crystals. Amer. Jour. Bot. 34: 42-52.
  10. Holechek, J.L.(1982) Sample preparation techniques for microhistological analysis. J. Range Manage. 35: 267-268. https://doi.org/10.2307/3898409
  11. Hu, J., H.J. Pan, Q.H. Wan and S.G. Fang(2005) Nuclear DNA microsatellite analysis of genetic diversity in captive populations of Chinese water deer. Small Rumin. Research(in press).
  12. Jerry, L., M.V. Holecheck and R.D. Rieper(1982) Botanical composition determination of range herbivore diets: A Review. Journal of Range Management 35: 309-315. https://doi.org/10.2307/3898308
  13. Johnson, M.K., H. Wofford and H.A. Pearson(1983) Microhistological Techniques for food habits analysis. USDA (United States Department of Agriculture), New Orleans, Louisiana, USA, 90pp.
  14. Katona, K., Z. Biro, Szemethy L, Demes T, Nyeste M. 2010. Spatial, temporal and individual variability in the autumn diet of european hare(Lepus europaeus) in Hungary. Acta Zoologica Academiae Scientiarum Hungaricae 56(1): 89-101.
  15. Kim, W.M., J.Y. Kim, C.W. Seo, E.K. Kim, J.H. Kim, J.H. Shin, K.J. Bang, and Y.G. Joe(2009) Study on the optimal management for sustainalbe use of Korean water deer population. National Institute of Environmental Research, 43pp.
  16. Korschgen, L.J.(1977) Histological characteristics of plant leaf epidermis and related structures as an aid in food habits studies. Fed. Aid Proj. Columbia, Mo.
  17. Lee, B.K.(2003) Morphological, ecological and DNA taxonomic characteristics of chinese water deer(Hydropotes inermis Swinhoe). Ph.D. thesis, Chungbuk national Univ., Rep. of Korea, pp. 1-101.
  18. Lieverloo, R.J.V., B.F. Schuiling, W.R.D. Boer, R.C. Lent, C.B.D. Jong, D. Brown and H.H.T. Prins(2009) A comparison of faecal analysis with backtracking to determine the diet composition and species preference of the black rhinoceros (Diceros bicornis minor). Eur. J. Wildl. Res. 55: 505-515. https://doi.org/10.1007/s10344-009-0264-5
  19. Litvaitis, J.A.(2000) Investigating food habits of terrestrial vertebrates. -In: Boitani, L.& Fuller, T.K. (Eds.); Research Techniques in Animal Ecology: Controversies and Consequences. Columbia University Press, New York, pp. 165-190.
  20. Marrero, P. and M. Nogales(2005) A microhistological survey on the trees of a relict subtropical laurel forest from the Macaronesian Islands as a base for assessing vertebrate plant diet. Botanical Journal of the Linnean Society 148: 409-426. https://doi.org/10.1111/j.1095-8339.2005.00411.x
  21. Metcalfe, C.R.(1960) Anatomy of the momocotyledons. Vol I. Gramineae. Oxford: Clarendon Press.
  22. Metcalfe, C.R. and L. Chalk(1950) Anatomy of the dicotyledons. 2Vols, Clarendon Press, Oxford, England, 150pp.
  23. Park, J.E., B.J. Kim and S.D. Lee(2010) A study of potential of diet analysis in the Korean water deer(Hydropotes inermis argyropus) using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE). Kor. J. Env. Eco. 24(3): 318-324.
  24. Ramirez, R.G., J.B. Quintanilla and J. Aranda(1997) White-tailed food habits in northeastern Mexico. Small Ruminant Research. 25: 141-146. https://doi.org/10.1016/S0921-4488(96)00960-1
  25. Rollins, R.C.(1944) Evidence for natural hybridity between quayule(Parthenum argentatum) and mariola(Parthenium incanum) Amer. Jour. Bot. 31: 93-99. https://doi.org/10.2307/2437599
  26. Sierra, P.V., M.S. Cid, M.A. Brizuela and C.M. Ferri(2005) Microhistological estiamtion of grass leaf blade percentages in pastures and diets. Rangeland Ecol Manage. 58: 207-214. https://doi.org/10.2111/1551-5028(2005)58<207:MEOGLB>2.0.CO;2
  27. Wang, S.(1998) China red data book of Endangered animal. Mammal Volume. Beijing: Science Press, In Chinese.
  28. Wegge, P., K. Shrestha and S.R. Moe(2006) Dry season diets of sympatric ungulates in lowland Nepal: competition and facilitation in alluvial tall grasslands. Ecol Res. 21: 698-706. https://doi.org/10.1007/s11284-006-0177-7