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Efficacy of Electrolyzed Water and Aqueous Chlorine Dioxide for Reducing Pathogenic Microorganism on Chinese Cabbage

전기분해수 및 이산화염소수 처리에 따른 배추의 미생물 제어 효과

  • Received : 2011.11.22
  • Accepted : 2012.01.20
  • Published : 2012.04.30

Abstract

This study evaluated the efficacy of strong acidic electrolyzed water (SAcEW), low alkaline electrolyzed water (LAlEW) and aqueous chlorine dioxide (ACD) for reducing pathogenic bacteria($Escherichia$ $coli$, $Bacillus$ $cereus$, $Salmonella$ Typhimurium, $Stapylococcus$ $aureus$) on Chinese cabbage. Artificially inoculated Chinese cabbage was immersed for 1, 5 and 10 min with TW, NaClO, EW and ACD. Generally, leaves showed more effective reduction than stems. Regarding the inhibitory effect, ACD treatment showed the highest effects rather than other treatments. When Chinese cabbage was immersed for 3 min in sterilized water, it was reduced to a minimum of 1.33 log CFU/g at LAlEW and a maximum of 4.70 log CFU/g at ACD. Compared to NaClO, ACD and LAlEW which showed a reduction of 3.2 log CFU/g ($Sal.$ Typhimurium) and 2.7 log CFU/g($B.$ $cereus$), respectively. Furthermore, the others had similar inhibitory effects compared to NaClO.

본 연구에서는 일반적인 염소소독법을 대체하기 위하여 살균소독수로의 유효성을 가진 강산성 및 약알칼리 전기분해수와 이산화염소수를 배추 세척에 적용하였다. 배추 표면에 $E.$ $coli$, $B.$ $cereus$, $Sal.$ Typhimurium, 및 $S.$ $aureus$를 인위적으로 오염시킨 후 각각의 살균소독수를 100 ppm의 농도로 하여 침지 시간을 달리하면서 미생물 제어효과를 비교하였다. 전체적으로 줄기 부분보다 잎 부분에서 세척 효과가 더 크게 나타났으며 네 가지 병원성 미생물에 대한 세척효과는 이산화염소수가 가장 큰 것으로 나타났다. 수도수 처리 시 10분이 경과하여도 1 log CFU/g 이하의 낮은 감소 효과가 나타났으며, 살균소독수에 3분 침지 하였을 때 최소 감소효과는 약알칼리 전기분해수에서 1.3 log CFU/g였으며, 최대 감소효과는 이산화염소수에서 4.7 log CFU/g의 수준으로 수도수 세척에 비해 살균소독수의 미생물 감소효과가 크게 나타났다. 또한 5분과 10분 처리구에서 유의성은 있었으나 그 차이가 크지 않아 배추의 미생물학적 위험을 감소시키기 위해서는 5분정도 침지 시키는 방법이 효과적일 것으로 생각된다. 차아염소산 나트륨수와 비교 시 산성 및 약알칼리 전기분해수의 미생물 감소효과는 유사하거나 최대 2.7 log CFU/g 정도(LAlEW, B. cereus, 10분)의 차이를 나타냈고, 이산화염소수는 3.2 log CFU/g ($Sal.$ Typhimurium, 10분) 수준의 차이를 나타내어 본 연구에서 사용된 살균소독수가 염소소독 대체재로서 가능성이 있는 것으로 사료된다. 또한 이산화염소수는 병원성 미생물을 제어하는 데는 우수한 것으로 평가되었으나 배추의 품질 저하가 야기되어 배추 적용에는 부적합한 것으로 판단된다.

Keywords

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